Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria
Heavy metals are a growing threat to human health due to the resulting damage to the ecology; the removal of heavy metals by lactic acid bacteria (LAB) has been a focus of many studies. In this study, 10 LAB strains were evaluated for their ability to absorb and tolerate lead. Lactobacillus plantaru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/22/5540 |
| _version_ | 1811200227696181248 |
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| author | Shaoli Liu Yi Zheng Yimiao Ma Abid Sarwar Xiao Zhao Tianqi Luo Zhennai Yang |
| author_facet | Shaoli Liu Yi Zheng Yimiao Ma Abid Sarwar Xiao Zhao Tianqi Luo Zhennai Yang |
| author_sort | Shaoli Liu |
| collection | DOAJ |
| description | Heavy metals are a growing threat to human health due to the resulting damage to the ecology; the removal of heavy metals by lactic acid bacteria (LAB) has been a focus of many studies. In this study, 10 LAB strains were evaluated for their ability to absorb and tolerate lead. Lactobacillus plantarum YW11 was found to possess the strongest ability of lead absorbing and tolerance, with the rate of absorption as high as 99.9% and the minimum inhibitory concentration of lead on YW11 higher than 1000 mg/L. Based on the isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis of YW11, a total of 2009 proteins were identified both in the lead-treated strain and the control without the lead treatment. Among these proteins, 44 different proteins were identified. The abundance of 25 proteins increased significantly, and 19 proteins decreased significantly in the treatment group. These significantly differential abundant proteins are involved in the biological processes of amino acid and lipid metabolism, energy metabolism, cell wall biosynthesis, and substance transport. This study contributed further understanding of the molecular mechanism of L. plantarum in the binding and removal of lead to explore its potential application in counteracting heavy metal pollution of environment, food, and other fields. |
| first_indexed | 2024-04-12T02:01:05Z |
| format | Article |
| id | doaj.art-294ee2c2a09740698389512e6575c595 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T02:01:05Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-294ee2c2a09740698389512e6575c5952022-12-22T03:52:40ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-11-012022554010.3390/ijms20225540ijms20225540Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid BacteriaShaoli Liu0Yi Zheng1Yimiao Ma2Abid Sarwar3Xiao Zhao4Tianqi Luo5Zhennai Yang6Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaHeavy metals are a growing threat to human health due to the resulting damage to the ecology; the removal of heavy metals by lactic acid bacteria (LAB) has been a focus of many studies. In this study, 10 LAB strains were evaluated for their ability to absorb and tolerate lead. Lactobacillus plantarum YW11 was found to possess the strongest ability of lead absorbing and tolerance, with the rate of absorption as high as 99.9% and the minimum inhibitory concentration of lead on YW11 higher than 1000 mg/L. Based on the isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis of YW11, a total of 2009 proteins were identified both in the lead-treated strain and the control without the lead treatment. Among these proteins, 44 different proteins were identified. The abundance of 25 proteins increased significantly, and 19 proteins decreased significantly in the treatment group. These significantly differential abundant proteins are involved in the biological processes of amino acid and lipid metabolism, energy metabolism, cell wall biosynthesis, and substance transport. This study contributed further understanding of the molecular mechanism of L. plantarum in the binding and removal of lead to explore its potential application in counteracting heavy metal pollution of environment, food, and other fields.https://www.mdpi.com/1422-0067/20/22/5540lactic acid bacteria<i>lactobacillus plantarum</i>leadabsorptionproteomics |
| spellingShingle | Shaoli Liu Yi Zheng Yimiao Ma Abid Sarwar Xiao Zhao Tianqi Luo Zhennai Yang Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria International Journal of Molecular Sciences lactic acid bacteria <i>lactobacillus plantarum</i> lead absorption proteomics |
| title | Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria |
| title_full | Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria |
| title_fullStr | Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria |
| title_full_unstemmed | Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria |
| title_short | Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria |
| title_sort | evaluation and proteomic analysis of lead adsorption by lactic acid bacteria |
| topic | lactic acid bacteria <i>lactobacillus plantarum</i> lead absorption proteomics |
| url | https://www.mdpi.com/1422-0067/20/22/5540 |
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