Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglycer...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2072-6643/13/12/4448 |
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| author | Woo Yong Park Gahee Song Joon Hak Noh Taegon Kim Jae Jin Kim Seokbeom Hong Jinbong Park Jae-Young Um |
| author_facet | Woo Yong Park Gahee Song Joon Hak Noh Taegon Kim Jae Jin Kim Seokbeom Hong Jinbong Park Jae-Young Um |
| author_sort | Woo Yong Park |
| collection | DOAJ |
| description | In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis. |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:23:10Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-294ef85fcdf44a86909285cc3b813a6a2023-11-23T09:57:50ZengMDPI AGNutrients2072-66432021-12-011312444810.3390/nu13124448Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo LipogenesisWoo Yong Park0Gahee Song1Joon Hak Noh2Taegon Kim3Jae Jin Kim4Seokbeom Hong5Jinbong Park6Jae-Young Um7Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaCollege of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaCollege of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaCollege of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Pharmacology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaIn this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis.https://www.mdpi.com/2072-6643/13/12/4448AFLDRaphani SemenlipogenesisSREBF1Lpin1 |
| spellingShingle | Woo Yong Park Gahee Song Joon Hak Noh Taegon Kim Jae Jin Kim Seokbeom Hong Jinbong Park Jae-Young Um Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis Nutrients AFLD Raphani Semen lipogenesis SREBF1 Lpin1 |
| title | Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
| title_full | Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
| title_fullStr | Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
| title_full_unstemmed | Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
| title_short | Raphani Semen (<i>Raphanus sativus</i> L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
| title_sort | raphani semen i raphanus sativus i l ameliorates alcoholic fatty liver disease by regulating de novo lipogenesis |
| topic | AFLD Raphani Semen lipogenesis SREBF1 Lpin1 |
| url | https://www.mdpi.com/2072-6643/13/12/4448 |
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