MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes
miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSC...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/18/4373 |
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| author | Heejin Lee Sang Hoon Chun Seo Yun Moon Jung-Sook Yoon Hye Sung Won Soon Auck Hong Seo Ree Kim Kwang-Jae Cho Keunsoo Kang Sieun Lee Young-Ho Ahn Ji Hyung Hong Yoon Ho Ko |
| author_facet | Heejin Lee Sang Hoon Chun Seo Yun Moon Jung-Sook Yoon Hye Sung Won Soon Auck Hong Seo Ree Kim Kwang-Jae Cho Keunsoo Kang Sieun Lee Young-Ho Ahn Ji Hyung Hong Yoon Ho Ko |
| author_sort | Heejin Lee |
| collection | DOAJ |
| description | miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients. |
| first_indexed | 2024-03-10T00:30:17Z |
| format | Article |
| id | doaj.art-29550da9aefb4433832a7b8e87a963bd |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T00:30:17Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-29550da9aefb4433832a7b8e87a963bd2023-11-23T15:26:10ZengMDPI AGCancers2072-66942022-09-011418437310.3390/cancers14184373MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal GenesHeejin Lee0Sang Hoon Chun1Seo Yun Moon2Jung-Sook Yoon3Hye Sung Won4Soon Auck Hong5Seo Ree Kim6Kwang-Jae Cho7Keunsoo Kang8Sieun Lee9Young-Ho Ahn10Ji Hyung Hong11Yoon Ho Ko12Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreaCancer Research Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreaUijeongbu St. Mary’s Hospital Clinical Research Laboratory, The Catholic University of Korea, Uijeongbu-si 11765, KoreaCancer Research Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreaDepartment of Pathology, College of Medicine, Chung-Ang University, Seoul 06973, KoreaDivision of Oncology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreaDepartment of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea College of Medicine, Seoul 06591, KoreaDepartment of Microbiology, College of Science & Technology, Dankook University, Cheonan 31116, KoreaDepartment of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul 07804, KoreaDepartment of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul 07804, KoreaCancer Research Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreaCancer Research Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, KoreamiR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.https://www.mdpi.com/2072-6694/14/18/4373miR-769head and neck neoplasmstromatumor-suppressiveEMT |
| spellingShingle | Heejin Lee Sang Hoon Chun Seo Yun Moon Jung-Sook Yoon Hye Sung Won Soon Auck Hong Seo Ree Kim Kwang-Jae Cho Keunsoo Kang Sieun Lee Young-Ho Ahn Ji Hyung Hong Yoon Ho Ko MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes Cancers miR-769 head and neck neoplasm stroma tumor-suppressive EMT |
| title | MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes |
| title_full | MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes |
| title_fullStr | MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes |
| title_full_unstemmed | MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes |
| title_short | MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes |
| title_sort | microrna 769 3p acts as a prognostic factor in oral squamous cell cancer by modulating stromal genes |
| topic | miR-769 head and neck neoplasm stroma tumor-suppressive EMT |
| url | https://www.mdpi.com/2072-6694/14/18/4373 |
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