Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate
Cholinesterase activity (ChA), the effective biomarker for organophosphate pesticide exposure, is possibly affected by single nucleotide polymorphisms (SNPs) in cell-cycle-related genes. One hundred and eighty workers with long-term exposure to omethoate and 115 healthy controls were recruited to ex...
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| Format: | Article |
| Language: | English |
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The Royal Society
2018-01-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.172357 |
| _version_ | 1819009317586599936 |
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| author | Xiaoran Duan Yongli Yang Sihua Wang Xiaolei Feng Tuanwei Wang Pengpeng Wang Wu Yao Liuxin Cui Wei Wang |
| author_facet | Xiaoran Duan Yongli Yang Sihua Wang Xiaolei Feng Tuanwei Wang Pengpeng Wang Wu Yao Liuxin Cui Wei Wang |
| author_sort | Xiaoran Duan |
| collection | DOAJ |
| description | Cholinesterase activity (ChA), the effective biomarker for organophosphate pesticide exposure, is possibly affected by single nucleotide polymorphisms (SNPs) in cell-cycle-related genes. One hundred and eighty workers with long-term exposure to omethoate and 115 healthy controls were recruited to explore the gene–gene and gene–environment interactions. The acetylthiocholine and dithio-bis-(nitrobenzoic acid) method was used to detect the cholinesterase activities in whole blood, erythrocytes and plasma. Genetic polymorphisms were determined by the PCR-RFLP and direct PCR electrophoresis methods. Statistical results showed that the cholinesterase activities of whole blood, erythrocytes and plasma in the exposure group were significantly lower than those in the control group (p < 0.001), and erythrocyte cholinesterase activities were associated with gender, smoking and drinking in the exposure group (p < 0.05). Single-locus analyses showed that there is a statistically significant difference in the ChA among the genotypes CC, CA and AA of the p21 rs1801270 locus in the control group (p = 0.033), but not in the exposure group. A significant interaction between genes and environmental factors (i.e. p53, p21, mdm2, gender, smoking and drinking) affecting ChA was found through a generalized multifactor dimensionality reduction analysis. These obtained markers will be useful in further marker-assisted selection in workers with exposure to omethoate. |
| first_indexed | 2024-12-21T00:54:27Z |
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| id | doaj.art-2959c5f9b0c54cb4bf1248e4afe4e295 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-21T00:54:27Z |
| publishDate | 2018-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-2959c5f9b0c54cb4bf1248e4afe4e2952022-12-21T19:21:20ZengThe Royal SocietyRoyal Society Open Science2054-57032018-01-015510.1098/rsos.172357172357Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoateXiaoran DuanYongli YangSihua WangXiaolei FengTuanwei WangPengpeng WangWu YaoLiuxin CuiWei WangCholinesterase activity (ChA), the effective biomarker for organophosphate pesticide exposure, is possibly affected by single nucleotide polymorphisms (SNPs) in cell-cycle-related genes. One hundred and eighty workers with long-term exposure to omethoate and 115 healthy controls were recruited to explore the gene–gene and gene–environment interactions. The acetylthiocholine and dithio-bis-(nitrobenzoic acid) method was used to detect the cholinesterase activities in whole blood, erythrocytes and plasma. Genetic polymorphisms were determined by the PCR-RFLP and direct PCR electrophoresis methods. Statistical results showed that the cholinesterase activities of whole blood, erythrocytes and plasma in the exposure group were significantly lower than those in the control group (p < 0.001), and erythrocyte cholinesterase activities were associated with gender, smoking and drinking in the exposure group (p < 0.05). Single-locus analyses showed that there is a statistically significant difference in the ChA among the genotypes CC, CA and AA of the p21 rs1801270 locus in the control group (p = 0.033), but not in the exposure group. A significant interaction between genes and environmental factors (i.e. p53, p21, mdm2, gender, smoking and drinking) affecting ChA was found through a generalized multifactor dimensionality reduction analysis. These obtained markers will be useful in further marker-assisted selection in workers with exposure to omethoate.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.172357generalized multifactor dimensionality reductionomethoatecholinesterase activitysingle nucleotide polymorphismsinteraction |
| spellingShingle | Xiaoran Duan Yongli Yang Sihua Wang Xiaolei Feng Tuanwei Wang Pengpeng Wang Wu Yao Liuxin Cui Wei Wang Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate Royal Society Open Science generalized multifactor dimensionality reduction omethoate cholinesterase activity single nucleotide polymorphisms interaction |
| title | Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate |
| title_full | Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate |
| title_fullStr | Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate |
| title_full_unstemmed | Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate |
| title_short | Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate |
| title_sort | interaction between polymorphisms in cell cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate |
| topic | generalized multifactor dimensionality reduction omethoate cholinesterase activity single nucleotide polymorphisms interaction |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.172357 |
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