Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF
Vascular endothelial growth factor (VEGF) is a critical biomarker in the angiogenesis of several cancers. Nowadays, novel approaches to rapid, sensitive, and reliable VEGF detection are urgently required for early cancer diagnosis. Cationic comb-type copolymer, poly(L-lysine)-<i>graft</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/5/262 |
| _version_ | 1797501222639370240 |
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| author | Jialun Han Chenxin Fang Ping Ouyang Yang Qing Yuxing Yang Haiyu Li Zhencui Wang Jie Du |
| author_facet | Jialun Han Chenxin Fang Ping Ouyang Yang Qing Yuxing Yang Haiyu Li Zhencui Wang Jie Du |
| author_sort | Jialun Han |
| collection | DOAJ |
| description | Vascular endothelial growth factor (VEGF) is a critical biomarker in the angiogenesis of several cancers. Nowadays, novel approaches to rapid, sensitive, and reliable VEGF detection are urgently required for early cancer diagnosis. Cationic comb-type copolymer, poly(L-lysine)-<i>graft</i>-dextran (PLL-<i>g</i>-Dex) accelerates DNA hybridization and chain exchange reaction while stabilizing the DNA assembly structure. In this work, we examined the chaperone activity of PLL-<i>g</i>-Dex to assist G-quadruplex-based fluorescent DNA biosensors for sensitive detection of VEGF. This convenient and effective strategy is based on chitosan hydrogel, c-myc, Thioflavin T (ThT), VEGF aptamer, and its partially complementary strand. The results show that chaperone copolymer PLL-<i>g</i>-Dex significantly promotes the accumulation of G-quadruplex and assembles into G-wires, allowing an effective signal amplification. Using this method, the detection limit of VEGF was as low as 23 pM, better than many previous works on aptamer-based VEGF detection. This chaperone copolymer-assisted signal amplification strategy has potential applications in the highly sensitive detection of target proteins, even including viruses. |
| first_indexed | 2024-03-10T03:15:17Z |
| format | Article |
| id | doaj.art-295df7b284e64b2cad2f01f854ea4316 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T03:15:17Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-295df7b284e64b2cad2f01f854ea43162023-11-23T10:14:56ZengMDPI AGBiosensors2079-63742022-04-0112526210.3390/bios12050262Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGFJialun Han0Chenxin Fang1Ping Ouyang2Yang Qing3Yuxing Yang4Haiyu Li5Zhencui Wang6Jie Du7College of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaVascular endothelial growth factor (VEGF) is a critical biomarker in the angiogenesis of several cancers. Nowadays, novel approaches to rapid, sensitive, and reliable VEGF detection are urgently required for early cancer diagnosis. Cationic comb-type copolymer, poly(L-lysine)-<i>graft</i>-dextran (PLL-<i>g</i>-Dex) accelerates DNA hybridization and chain exchange reaction while stabilizing the DNA assembly structure. In this work, we examined the chaperone activity of PLL-<i>g</i>-Dex to assist G-quadruplex-based fluorescent DNA biosensors for sensitive detection of VEGF. This convenient and effective strategy is based on chitosan hydrogel, c-myc, Thioflavin T (ThT), VEGF aptamer, and its partially complementary strand. The results show that chaperone copolymer PLL-<i>g</i>-Dex significantly promotes the accumulation of G-quadruplex and assembles into G-wires, allowing an effective signal amplification. Using this method, the detection limit of VEGF was as low as 23 pM, better than many previous works on aptamer-based VEGF detection. This chaperone copolymer-assisted signal amplification strategy has potential applications in the highly sensitive detection of target proteins, even including viruses.https://www.mdpi.com/2079-6374/12/5/262signal amplificationchaperone copolymerG-quadruplexVEGF detection |
| spellingShingle | Jialun Han Chenxin Fang Ping Ouyang Yang Qing Yuxing Yang Haiyu Li Zhencui Wang Jie Du Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF Biosensors signal amplification chaperone copolymer G-quadruplex VEGF detection |
| title | Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF |
| title_full | Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF |
| title_fullStr | Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF |
| title_full_unstemmed | Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF |
| title_short | Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF |
| title_sort | chaperone copolymer assisted g quadruplex based signal amplification assay for highly sensitive detection of vegf |
| topic | signal amplification chaperone copolymer G-quadruplex VEGF detection |
| url | https://www.mdpi.com/2079-6374/12/5/262 |
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