β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells
The mechanisms of acute kidney injury and chronic kidney disease remain incompletely revealed, and drug development is a pressing clinical challenge. Oxidative stress-induced cellular senescence and mitochondrial damage are important biological events in a variety of kidney diseases. As a type of ca...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Ye Zhang Hu Mao Yanze Li Yufeng Xiong Xiuheng Liu Lei Wang Zhiyuan Chen |
| author_facet | Ye Zhang Hu Mao Yanze Li Yufeng Xiong Xiuheng Liu Lei Wang Zhiyuan Chen |
| author_sort | Ye Zhang |
| collection | DOAJ |
| description | The mechanisms of acute kidney injury and chronic kidney disease remain incompletely revealed, and drug development is a pressing clinical challenge. Oxidative stress-induced cellular senescence and mitochondrial damage are important biological events in a variety of kidney diseases. As a type of carotenoid, β-Cryptoxanthin (BCX) has various biological functions, which means it is a potential therapeutic candidate for the treatment of kidney disease. However, the role of BCX in the kidney is unclear, and the effect of BCX on oxidative stress and cellular senescence in renal cells is also unknown. Therefore, we conducted a series of studies on human renal tubular epithelial (HK-2) cells in vitro. In the present study, we investigated the effect of BCX pretreatment on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and cellular senescence and explored the potential mechanism of BCX action. The results showed that BCX attenuated H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and cellular senescence in HK-2 cells. Moreover, BCX promoted NRF2 nuclear expression, maintained mitochondrial function, and reduced mitochondrial damage in HK-2 cells. In addition, silencing NRF2 altered the protective effect of BCX on mitochondria and significantly reversed the anti-oxidative stress and anti-senescence effects of BCX in HK-2 cells. We concluded that BCX maintained mitochondrial function by promoting NRF2 nuclear translocation to inhibit oxidative stress-induced senescence in HK-2 cells. In light of these findings, the application of BCX might be a promising strategy for the prevention and treatment of kidney diseases. |
| first_indexed | 2024-03-11T08:41:39Z |
| format | Article |
| id | doaj.art-2963f41a68294318b41a8505e7092b5b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:41:39Z |
| publishDate | 2023-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2963f41a68294318b41a8505e7092b5b2023-11-16T21:05:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244385110.3390/ijms24043851β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 CellsYe Zhang0Hu Mao1Yanze Li2Yufeng Xiong3Xiuheng Liu4Lei Wang5Zhiyuan Chen6Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaThe mechanisms of acute kidney injury and chronic kidney disease remain incompletely revealed, and drug development is a pressing clinical challenge. Oxidative stress-induced cellular senescence and mitochondrial damage are important biological events in a variety of kidney diseases. As a type of carotenoid, β-Cryptoxanthin (BCX) has various biological functions, which means it is a potential therapeutic candidate for the treatment of kidney disease. However, the role of BCX in the kidney is unclear, and the effect of BCX on oxidative stress and cellular senescence in renal cells is also unknown. Therefore, we conducted a series of studies on human renal tubular epithelial (HK-2) cells in vitro. In the present study, we investigated the effect of BCX pretreatment on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and cellular senescence and explored the potential mechanism of BCX action. The results showed that BCX attenuated H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and cellular senescence in HK-2 cells. Moreover, BCX promoted NRF2 nuclear expression, maintained mitochondrial function, and reduced mitochondrial damage in HK-2 cells. In addition, silencing NRF2 altered the protective effect of BCX on mitochondria and significantly reversed the anti-oxidative stress and anti-senescence effects of BCX in HK-2 cells. We concluded that BCX maintained mitochondrial function by promoting NRF2 nuclear translocation to inhibit oxidative stress-induced senescence in HK-2 cells. In light of these findings, the application of BCX might be a promising strategy for the prevention and treatment of kidney diseases.https://www.mdpi.com/1422-0067/24/4/3851oxidative stresssenescenceβ-CryptoxanthinNRF2HK-2 cells |
| spellingShingle | Ye Zhang Hu Mao Yanze Li Yufeng Xiong Xiuheng Liu Lei Wang Zhiyuan Chen β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells International Journal of Molecular Sciences oxidative stress senescence β-Cryptoxanthin NRF2 HK-2 cells |
| title | β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells |
| title_full | β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells |
| title_fullStr | β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells |
| title_full_unstemmed | β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells |
| title_short | β-Cryptoxanthin Maintains Mitochondrial Function by Promoting NRF2 Nuclear Translocation to Inhibit Oxidative Stress-Induced Senescence in HK-2 Cells |
| title_sort | β cryptoxanthin maintains mitochondrial function by promoting nrf2 nuclear translocation to inhibit oxidative stress induced senescence in hk 2 cells |
| topic | oxidative stress senescence β-Cryptoxanthin NRF2 HK-2 cells |
| url | https://www.mdpi.com/1422-0067/24/4/3851 |
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