PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT
The study aimed to identify the species origin of imported buffalo meat from three countries Ukraine, Brazil and India to Erbil using PCR-RFLP technique. The pair of universal cyt b primer was designed to amplify a 359 bp DNA fragment in PCR amplification. Then the amplified fragments were digested...
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| Format: | Article |
| Language: | Arabic |
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College of Agriculture
2020-12-01
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| Series: | Mesopotamia Journal of Agriculture |
| Subjects: | |
| Online Access: | https://magrj.mosuljournals.com/article_167383_c0678d6bbf7a049cff78177cd8e94a3a.pdf |
| _version_ | 1819283084077432832 |
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| author | Dilger Khdr Kamaran Taha Azad Sabow |
| author_facet | Dilger Khdr Kamaran Taha Azad Sabow |
| author_sort | Dilger Khdr |
| collection | DOAJ |
| description | The study aimed to identify the species origin of imported buffalo meat from three countries Ukraine, Brazil and India to Erbil using PCR-RFLP technique. The pair of universal cyt b primer was designed to amplify a 359 bp DNA fragment in PCR amplification. Then the amplified fragments were digested with Hinf1 and Rsa I restriction enzymes, achieving a characteristics banding pattern in a 2% agarose which produced evidence to identify origin meat species. The results presented that digestion of samples with the Hinf1 RE, produced two bands in each (Ukraine and Brazil), (58 and 301) bp while it was showed different bands in Indian buffalo meat (85, 274) bp. On the other hand the outcomes in the Rsa I RE were two bands in Ukraine and Brazil (156, 203) bp and two bands were obtained in Indian buffalo meat (106, 253) bp. The results realized that the Indian buffalo meat species was not acceptable and showed mislabeling products. Thus, the obtained results recommend that the PCR-RFLP technique with <em>HinfI</em> and <em>Rsa I</em> restriction enzymes play an important role to detect the origin meat species, since it is a fast, simple and easily handle technique for detection of meat species. |
| first_indexed | 2024-12-24T01:25:51Z |
| format | Article |
| id | doaj.art-296479b1bdcc402e9cb6c3dbeaabc67b |
| institution | Directory Open Access Journal |
| issn | 1815-316X 2224-9796 |
| language | Arabic |
| last_indexed | 2024-12-24T01:25:51Z |
| publishDate | 2020-12-01 |
| publisher | College of Agriculture |
| record_format | Article |
| series | Mesopotamia Journal of Agriculture |
| spelling | doaj.art-296479b1bdcc402e9cb6c3dbeaabc67b2022-12-21T17:22:30ZaraCollege of AgricultureMesopotamia Journal of Agriculture1815-316X2224-97962020-12-0148410411310.33899/magrj.2020.128802.1086167383PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEATDilger Khdr0Kamaran Taha1Azad Sabow2Animal Resources Dept. College of Agricultural Engineering Sciences, Salahaddin University, ErbilAnimal Resources Department, College of Agricultural Engineering Sciences, Salahaddin University, ErbilUniversity of Salahaddin: Erbil, Kurdistan Region, IraqThe study aimed to identify the species origin of imported buffalo meat from three countries Ukraine, Brazil and India to Erbil using PCR-RFLP technique. The pair of universal cyt b primer was designed to amplify a 359 bp DNA fragment in PCR amplification. Then the amplified fragments were digested with Hinf1 and Rsa I restriction enzymes, achieving a characteristics banding pattern in a 2% agarose which produced evidence to identify origin meat species. The results presented that digestion of samples with the Hinf1 RE, produced two bands in each (Ukraine and Brazil), (58 and 301) bp while it was showed different bands in Indian buffalo meat (85, 274) bp. On the other hand the outcomes in the Rsa I RE were two bands in Ukraine and Brazil (156, 203) bp and two bands were obtained in Indian buffalo meat (106, 253) bp. The results realized that the Indian buffalo meat species was not acceptable and showed mislabeling products. Thus, the obtained results recommend that the PCR-RFLP technique with <em>HinfI</em> and <em>Rsa I</em> restriction enzymes play an important role to detect the origin meat species, since it is a fast, simple and easily handle technique for detection of meat species.https://magrj.mosuljournals.com/article_167383_c0678d6bbf7a049cff78177cd8e94a3a.pdfimported buffalo meat,,,،,؛meat products identification,,,،,؛mt cyt b gene,,,،,؛restriction enzyme |
| spellingShingle | Dilger Khdr Kamaran Taha Azad Sabow PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT Mesopotamia Journal of Agriculture imported buffalo meat,, ,،,؛meat products identification,, ,،,؛mt cyt b gene,, ,،,؛restriction enzyme |
| title | PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT |
| title_full | PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT |
| title_fullStr | PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT |
| title_full_unstemmed | PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT |
| title_short | PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT |
| title_sort | pcr rflp technique for species origin identification of imported buffalo meat |
| topic | imported buffalo meat,, ,،,؛meat products identification,, ,،,؛mt cyt b gene,, ,،,؛restriction enzyme |
| url | https://magrj.mosuljournals.com/article_167383_c0678d6bbf7a049cff78177cd8e94a3a.pdf |
| work_keys_str_mv | AT dilgerkhdr pcrrflptechniqueforspeciesoriginidentificationofimportedbuffalomeat AT kamarantaha pcrrflptechniqueforspeciesoriginidentificationofimportedbuffalomeat AT azadsabow pcrrflptechniqueforspeciesoriginidentificationofimportedbuffalomeat |