Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS
The study aimed at analyzing the metabolite profile of Nardostachys jatamansi using RP-HPLC, GC-MS and also its antioxidant, biomolecule protective and cytoprotective properties. The 70% ethanolic extract of Nardostachys jatamansi (NJE) showed the presence of polyphenols and flavonoids (gallic acid,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-03-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/4/1/185 |
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| author | Sakina Razack Kandikattu Hemanth Kumar Ilaiyaraja Nallamuthu Mahadeva Naika Farhath Khanum |
| author_facet | Sakina Razack Kandikattu Hemanth Kumar Ilaiyaraja Nallamuthu Mahadeva Naika Farhath Khanum |
| author_sort | Sakina Razack |
| collection | DOAJ |
| description | The study aimed at analyzing the metabolite profile of Nardostachys jatamansi using RP-HPLC, GC-MS and also its antioxidant, biomolecule protective and cytoprotective properties. The 70% ethanolic extract of Nardostachys jatamansi (NJE) showed the presence of polyphenols and flavonoids (gallic acid, catechin, chlorogenic acid, homovanillin, epicatechin, rutin hydrate and quercetin-3-rhamnoside) analyzed by RP-HPLC, whereas hexane extract revealed an array of metabolites (fatty acids, sesquiterpenes, alkane hydrocarbons and esters) by GC-MS analysis. The antioxidant assays showed the enhanced potency of NJE with a half maximal inhibitory concentration (IC50) value of 222.22 ± 7.4 μg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 13.90 ± 0.5 μg/mL for 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 113.81 ± 4.2 μg/mL for superoxide, 948 ± 21.1 μg/mL for metal chelating and 12.3 ± 0.43 mg FeSO4 equivalent/g of extract for ferric reducing antioxidant power assays and was more potent than hexane extract. NJE effectively inhibited 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidation of biomolecules analyzed by pBR322 plasmid DNA damage, protein oxidation of bovine serum albumin and lipid peroxidation assays. The observed effects might be due to the high content of polyphenols, 53.06 ± 2.2 mg gallic acid equivalents/g, and flavonoids, 25.303 ± 0.9 mg catechin equivalents/g, of NJE compared to the hexane fraction. Additionally, the extract abrogated the protein, carbonyl, and ROS formation, and NJE showed cytotoxicity in SH-SY5Y neuronal cells above 75 μg/mL. Thus, the study suggests that the herb unequivocally is a potential source of antioxidants and could aid in alleviating oxidative stress-mediated disorders. |
| first_indexed | 2024-03-12T19:50:48Z |
| format | Article |
| id | doaj.art-2965169ee190480fb912fa6461901ae3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T19:50:48Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-2965169ee190480fb912fa6461901ae32023-08-02T03:08:53ZengMDPI AGAntioxidants2076-39212015-03-014118520310.3390/antiox4010185antiox4010185Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MSSakina Razack0Kandikattu Hemanth Kumar1Ilaiyaraja Nallamuthu2Mahadeva Naika3Farhath Khanum4Biochemistry and Nanosciences Division, Defence Food Research Laboratory, Mysore-570011, IndiaBiochemistry and Nanosciences Division, Defence Food Research Laboratory, Mysore-570011, IndiaBiochemistry and Nanosciences Division, Defence Food Research Laboratory, Mysore-570011, IndiaApplied Nutrition Division, Defence Food Research Laboratory, Mysore-570011, IndiaBiochemistry and Nanosciences Division, Defence Food Research Laboratory, Mysore-570011, IndiaThe study aimed at analyzing the metabolite profile of Nardostachys jatamansi using RP-HPLC, GC-MS and also its antioxidant, biomolecule protective and cytoprotective properties. The 70% ethanolic extract of Nardostachys jatamansi (NJE) showed the presence of polyphenols and flavonoids (gallic acid, catechin, chlorogenic acid, homovanillin, epicatechin, rutin hydrate and quercetin-3-rhamnoside) analyzed by RP-HPLC, whereas hexane extract revealed an array of metabolites (fatty acids, sesquiterpenes, alkane hydrocarbons and esters) by GC-MS analysis. The antioxidant assays showed the enhanced potency of NJE with a half maximal inhibitory concentration (IC50) value of 222.22 ± 7.4 μg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 13.90 ± 0.5 μg/mL for 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 113.81 ± 4.2 μg/mL for superoxide, 948 ± 21.1 μg/mL for metal chelating and 12.3 ± 0.43 mg FeSO4 equivalent/g of extract for ferric reducing antioxidant power assays and was more potent than hexane extract. NJE effectively inhibited 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidation of biomolecules analyzed by pBR322 plasmid DNA damage, protein oxidation of bovine serum albumin and lipid peroxidation assays. The observed effects might be due to the high content of polyphenols, 53.06 ± 2.2 mg gallic acid equivalents/g, and flavonoids, 25.303 ± 0.9 mg catechin equivalents/g, of NJE compared to the hexane fraction. Additionally, the extract abrogated the protein, carbonyl, and ROS formation, and NJE showed cytotoxicity in SH-SY5Y neuronal cells above 75 μg/mL. Thus, the study suggests that the herb unequivocally is a potential source of antioxidants and could aid in alleviating oxidative stress-mediated disorders.http://www.mdpi.com/2076-3921/4/1/185antioxidant activityDNA damage protective GC-MSNardostachys jatamansiphytochemical analysisRP-HPLC |
| spellingShingle | Sakina Razack Kandikattu Hemanth Kumar Ilaiyaraja Nallamuthu Mahadeva Naika Farhath Khanum Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS Antioxidants antioxidant activity DNA damage protective GC-MS Nardostachys jatamansi phytochemical analysis RP-HPLC |
| title | Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS |
| title_full | Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS |
| title_fullStr | Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS |
| title_full_unstemmed | Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS |
| title_short | Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS |
| title_sort | antioxidant biomolecule oxidation protective activities of nardostachys jatamansi dc and its phytochemical analysis by rp hplc and gc ms |
| topic | antioxidant activity DNA damage protective GC-MS Nardostachys jatamansi phytochemical analysis RP-HPLC |
| url | http://www.mdpi.com/2076-3921/4/1/185 |
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