Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content
The high deposition of intramuscular fat and the content of oleic fatty acid are characteristic of the Iberian pig. These two parameters present great variability and are differentiated amongst the varieties that make up the Iberian pig population. Although previous studies generated evidence for ca...
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MDPI AG
2021-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/627 |
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| author | Ana Villaplana-Velasco Jose Luis Noguera Ramona Natacha Pena Maria Ballester Lourdes Muñoz Elena González Juan Florencio Tejeda Noelia Ibáñez-Escriche |
| author_facet | Ana Villaplana-Velasco Jose Luis Noguera Ramona Natacha Pena Maria Ballester Lourdes Muñoz Elena González Juan Florencio Tejeda Noelia Ibáñez-Escriche |
| author_sort | Ana Villaplana-Velasco |
| collection | DOAJ |
| description | The high deposition of intramuscular fat and the content of oleic fatty acid are characteristic of the Iberian pig. These two parameters present great variability and are differentiated amongst the varieties that make up the Iberian pig population. Although previous studies generated evidence for causal genes and polymorphisms associated to the adipogenic potential of the Iberian pig, there is little information about how genetic expression influences this trait’s variability. The aim of this study was to analyses the expression profile between two varieties of Iberian pig (Torbiscal and Retinto) and their reciprocal crosses differentiated in their intramuscular fat (IMF) content and fatty acid (FA) composition in the <i>Longissimus thoracis</i> muscle using an RNA-seq approach. Our results corroborate that the Retinto variety is the fattiest amongst all studied varieties as its upregulated genes, such as <i>FABP3</i> and <i>FABP5</i>, <i>SLC27A1</i> and <i>VEGFA</i> among others, contribute to increasing adiposity. In its turn, Torbiscal pigs showed an upregulation of genes associated with the inhibition of fat deposition such as <i>ADIPOQ</i> and <i>CPT1A</i>. Further genetic variation analysis in these Iberian varieties showed relevant associations for SNP located within the differentially expressed genes with IMF and FA content. Thus, the differences found in the genetic architecture and the muscle transcriptome of these Iberian varieties might explain the variability in their fat content and composition and hence, their meat quality. |
| first_indexed | 2024-03-09T06:20:30Z |
| format | Article |
| id | doaj.art-2965c134e08f4feeb0f70144d3e2cede |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T06:20:30Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-2965c134e08f4feeb0f70144d3e2cede2023-12-03T11:47:53ZengMDPI AGAnimals2076-26152021-02-0111362710.3390/ani11030627Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids ContentAna Villaplana-Velasco0Jose Luis Noguera1Ramona Natacha Pena2Maria Ballester3Lourdes Muñoz4Elena González5Juan Florencio Tejeda6Noelia Ibáñez-Escriche7Genetics and Genomics, The Roslin Institute, Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush Campus, Midlothian, Edinburgh EH25 9RG, UKAnimal Breeding and Genetics Program, IRTA, 25198 Lleida, SpainDepartament de Ciència Animal, Universitat de Lleida-Agrotecnio Center, 25198 Lleida, SpainAnimal Breeding and Genetics Program, IRTA, Torre Marimon, 08140 Caldes de Montbui, SpainINGA FOOD S.A, 06200 Almendralejo, SpainDepartment of Animal Production and Food Science, Research University Institute of Agricultural Resources (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, 06007 Badajoz, SpainDepartment of Animal Production and Food Science, Research University Institute of Agricultural Resources (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, 06007 Badajoz, SpainDepartment for Animal Science and Tecnology, Universistat Politécnica de València, 46022 Valencia, SpainThe high deposition of intramuscular fat and the content of oleic fatty acid are characteristic of the Iberian pig. These two parameters present great variability and are differentiated amongst the varieties that make up the Iberian pig population. Although previous studies generated evidence for causal genes and polymorphisms associated to the adipogenic potential of the Iberian pig, there is little information about how genetic expression influences this trait’s variability. The aim of this study was to analyses the expression profile between two varieties of Iberian pig (Torbiscal and Retinto) and their reciprocal crosses differentiated in their intramuscular fat (IMF) content and fatty acid (FA) composition in the <i>Longissimus thoracis</i> muscle using an RNA-seq approach. Our results corroborate that the Retinto variety is the fattiest amongst all studied varieties as its upregulated genes, such as <i>FABP3</i> and <i>FABP5</i>, <i>SLC27A1</i> and <i>VEGFA</i> among others, contribute to increasing adiposity. In its turn, Torbiscal pigs showed an upregulation of genes associated with the inhibition of fat deposition such as <i>ADIPOQ</i> and <i>CPT1A</i>. Further genetic variation analysis in these Iberian varieties showed relevant associations for SNP located within the differentially expressed genes with IMF and FA content. Thus, the differences found in the genetic architecture and the muscle transcriptome of these Iberian varieties might explain the variability in their fat content and composition and hence, their meat quality.https://www.mdpi.com/2076-2615/11/3/627transcriptomicsIberian pigadipogenic potentialmeat qualityRNA-seq |
| spellingShingle | Ana Villaplana-Velasco Jose Luis Noguera Ramona Natacha Pena Maria Ballester Lourdes Muñoz Elena González Juan Florencio Tejeda Noelia Ibáñez-Escriche Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content Animals transcriptomics Iberian pig adipogenic potential meat quality RNA-seq |
| title | Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content |
| title_full | Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content |
| title_fullStr | Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content |
| title_full_unstemmed | Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content |
| title_short | Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content |
| title_sort | comparative transcriptome profile between iberian pig varieties provides new insights into their distinct fat deposition and fatty acids content |
| topic | transcriptomics Iberian pig adipogenic potential meat quality RNA-seq |
| url | https://www.mdpi.com/2076-2615/11/3/627 |
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