The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS.
Serotonin (5-HT) is a central inhibitor of food intake in mammals. Thus far, the intracellular mechanisms for the effect of serotonin on appetite regulation remain unclear. It has been recently demonstrated that reactive oxygen species (ROS) in the hypothalamus are a crucial integrative target for t...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3541393?pdf=render |
| _version_ | 1811212216094949376 |
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| author | Xin-Ling Fang Gang Shu Jian-Jian Yu Li-Na Wang Jing Yang Qing-Jie Zeng Xiao Cheng Zhi-Qi Zhang Song-Bo Wang Ping Gao Xiao-Tong Zhu Qian-Yun Xi Yong-Liang Zhang Qing-Yan Jiang |
| author_facet | Xin-Ling Fang Gang Shu Jian-Jian Yu Li-Na Wang Jing Yang Qing-Jie Zeng Xiao Cheng Zhi-Qi Zhang Song-Bo Wang Ping Gao Xiao-Tong Zhu Qian-Yun Xi Yong-Liang Zhang Qing-Yan Jiang |
| author_sort | Xin-Ling Fang |
| collection | DOAJ |
| description | Serotonin (5-HT) is a central inhibitor of food intake in mammals. Thus far, the intracellular mechanisms for the effect of serotonin on appetite regulation remain unclear. It has been recently demonstrated that reactive oxygen species (ROS) in the hypothalamus are a crucial integrative target for the regulation of food intake. To investigate the role of ROS in the serotonin-induced anorexigenic effects, conscious mice were treated with 5-HT alone or combination with Trolox (a ROS scavenger) or Apocynin (an NADPH oxidase inhibitor) by acute intracerebroventricular injection. Both Trolox and Apocynin reversed the anorexigenic action of 5-HT and the 5-HT-induced hypothalamic ROS elevation. The mRNA and protein expression levels of pro-opiomelanocortin (POMC) were dramatically increased after ICV injection with 5-HT. The anorexigenic action of 5-HT was accompanied by markedly elevated hypothalamic MDA levels and GSH-Px activity, while the SOD activity was decreased. Moreover, 5-HT significantly increased the mRNA expression of UCP-2 but reduced the levels of UCP-3. Both Trolox and Apocynin could block the 5-HT-induced changes in UCP-2 and UCP-3 gene expression. Our study demonstrates for the first time that the anorexigenic effect of 5-HT is mediated by the generation of ROS in the hypothalamus through an NADPH oxidase-dependent pathway. |
| first_indexed | 2024-04-12T05:25:25Z |
| format | Article |
| id | doaj.art-296789643e4042d2861283959a5caf3a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:25:25Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-296789643e4042d2861283959a5caf3a2022-12-22T03:46:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5314210.1371/journal.pone.0053142The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS.Xin-Ling FangGang ShuJian-Jian YuLi-Na WangJing YangQing-Jie ZengXiao ChengZhi-Qi ZhangSong-Bo WangPing GaoXiao-Tong ZhuQian-Yun XiYong-Liang ZhangQing-Yan JiangSerotonin (5-HT) is a central inhibitor of food intake in mammals. Thus far, the intracellular mechanisms for the effect of serotonin on appetite regulation remain unclear. It has been recently demonstrated that reactive oxygen species (ROS) in the hypothalamus are a crucial integrative target for the regulation of food intake. To investigate the role of ROS in the serotonin-induced anorexigenic effects, conscious mice were treated with 5-HT alone or combination with Trolox (a ROS scavenger) or Apocynin (an NADPH oxidase inhibitor) by acute intracerebroventricular injection. Both Trolox and Apocynin reversed the anorexigenic action of 5-HT and the 5-HT-induced hypothalamic ROS elevation. The mRNA and protein expression levels of pro-opiomelanocortin (POMC) were dramatically increased after ICV injection with 5-HT. The anorexigenic action of 5-HT was accompanied by markedly elevated hypothalamic MDA levels and GSH-Px activity, while the SOD activity was decreased. Moreover, 5-HT significantly increased the mRNA expression of UCP-2 but reduced the levels of UCP-3. Both Trolox and Apocynin could block the 5-HT-induced changes in UCP-2 and UCP-3 gene expression. Our study demonstrates for the first time that the anorexigenic effect of 5-HT is mediated by the generation of ROS in the hypothalamus through an NADPH oxidase-dependent pathway.http://europepmc.org/articles/PMC3541393?pdf=render |
| spellingShingle | Xin-Ling Fang Gang Shu Jian-Jian Yu Li-Na Wang Jing Yang Qing-Jie Zeng Xiao Cheng Zhi-Qi Zhang Song-Bo Wang Ping Gao Xiao-Tong Zhu Qian-Yun Xi Yong-Liang Zhang Qing-Yan Jiang The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS. PLoS ONE |
| title | The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS. |
| title_full | The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS. |
| title_fullStr | The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS. |
| title_full_unstemmed | The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS. |
| title_short | The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS. |
| title_sort | anorexigenic effect of serotonin is mediated by the generation of nadph oxidase dependent ros |
| url | http://europepmc.org/articles/PMC3541393?pdf=render |
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