Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)

以酿酒酵母耐热菌株HU-TY-1和亲株LK为材料,通过SDS-聚丙烯酰胺凝胶电泳等技术,对它们在热休克应答前后的耐热性及蛋白图谱进行了分析,发现两个菌株经温和高温(37 ℃)诱导后可大幅提高对致死温度(53 ℃)的耐受力,同时伴随着分子量为72 kD和84 kD的热击蛋白(HSP)的合成,其中耐热株HU-TY-1在常态时就有这两种热击蛋白的少量表达.由此表明:耐热酵母菌株HU-TY-1的高耐热性与HSP72和HSP84的组成性合成密切相关....

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Main Authors: JINCheng-tao(金承涛), ZENGYun-zhong(曾云中), WUXue-chang(吴雪昌), ZHUXu-fen(朱旭芬)
Format: Article
Language:zho
Published: Zhejiang University Press 2001-11-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
Online Access:https://doi.org/zjup/1008-9497.2001.28.6.676-681
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author JINCheng-tao(金承涛)
ZENGYun-zhong(曾云中)
WUXue-chang(吴雪昌)
ZHUXu-fen(朱旭芬)
author_facet JINCheng-tao(金承涛)
ZENGYun-zhong(曾云中)
WUXue-chang(吴雪昌)
ZHUXu-fen(朱旭芬)
author_sort JINCheng-tao(金承涛)
collection DOAJ
description 以酿酒酵母耐热菌株HU-TY-1和亲株LK为材料,通过SDS-聚丙烯酰胺凝胶电泳等技术,对它们在热休克应答前后的耐热性及蛋白图谱进行了分析,发现两个菌株经温和高温(37 ℃)诱导后可大幅提高对致死温度(53 ℃)的耐受力,同时伴随着分子量为72 kD和84 kD的热击蛋白(HSP)的合成,其中耐热株HU-TY-1在常态时就有这两种热击蛋白的少量表达.由此表明:耐热酵母菌株HU-TY-1的高耐热性与HSP72和HSP84的组成性合成密切相关.
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spelling doaj.art-296bb9cce9b84b56895690dbc0c6d7a52024-03-29T01:58:17ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972001-11-01286676681zjup/1008-9497.2001.28.6.676-681Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)JINCheng-tao(金承涛)0ZENGYun-zhong(曾云中)1WUXue-chang(吴雪昌)2ZHUXu-fen(朱旭芬)3College of Life Science, Zhejiang University, Hangzhou 310028, China(浙江大学生命科学学院,浙江 杭州 310028)College of Life Science, Zhejiang University, Hangzhou 310028, China(浙江大学生命科学学院,浙江 杭州 310028)College of Life Science, Zhejiang University, Hangzhou 310028, China(浙江大学生命科学学院,浙江 杭州 310028)College of Life Science, Zhejiang University, Hangzhou 310028, China(浙江大学生命科学学院,浙江 杭州 310028)以酿酒酵母耐热菌株HU-TY-1和亲株LK为材料,通过SDS-聚丙烯酰胺凝胶电泳等技术,对它们在热休克应答前后的耐热性及蛋白图谱进行了分析,发现两个菌株经温和高温(37 ℃)诱导后可大幅提高对致死温度(53 ℃)的耐受力,同时伴随着分子量为72 kD和84 kD的热击蛋白(HSP)的合成,其中耐热株HU-TY-1在常态时就有这两种热击蛋白的少量表达.由此表明:耐热酵母菌株HU-TY-1的高耐热性与HSP72和HSP84的组成性合成密切相关.https://doi.org/zjup/1008-9497.2001.28.6.676-681酿酒酵母热击蛋白热休克应答耐热机理
spellingShingle JINCheng-tao(金承涛)
ZENGYun-zhong(曾云中)
WUXue-chang(吴雪昌)
ZHUXu-fen(朱旭芬)
Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
Zhejiang Daxue xuebao. Lixue ban
酿酒酵母
热击蛋白
热休克应答
耐热机理
title Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
title_full Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
title_fullStr Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
title_full_unstemmed Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
title_short Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
title_sort study on heat shock protein and thermotolerant mechanism of s cerevisiae 耐热酵母菌株hu ty 1的耐热机理初探
topic 酿酒酵母
热击蛋白
热休克应答
耐热机理
url https://doi.org/zjup/1008-9497.2001.28.6.676-681
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