Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)
以酿酒酵母耐热菌株HU-TY-1和亲株LK为材料,通过SDS-聚丙烯酰胺凝胶电泳等技术,对它们在热休克应答前后的耐热性及蛋白图谱进行了分析,发现两个菌株经温和高温(37 ℃)诱导后可大幅提高对致死温度(53 ℃)的耐受力,同时伴随着分子量为72 kD和84 kD的热击蛋白(HSP)的合成,其中耐热株HU-TY-1在常态时就有这两种热击蛋白的少量表达.由此表明:耐热酵母菌株HU-TY-1的高耐热性与HSP72和HSP84的组成性合成密切相关....
Main Authors: | JINCheng-tao(金承涛), ZENGYun-zhong(曾云中), WUXue-chang(吴雪昌), ZHUXu-fen(朱旭芬) |
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Format: | Article |
Language: | zho |
Published: |
Zhejiang University Press
2001-11-01
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Series: | Zhejiang Daxue xuebao. Lixue ban |
Subjects: | |
Online Access: | https://doi.org/zjup/1008-9497.2001.28.6.676-681 |
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