Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea
PM2.5 (particulate matter with aerodynamic diameter ≤ 2.5 μm) is a well-known cytotoxic pollutant that capable to induce severe intracellular oxidative stress while the underlying mechanisms remain unclear. Herein, 4 types of PM2.5 derived from solid fuel burning were selected as stimuli in A549 cel...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412022006018 |
| _version_ | 1797948297832300544 |
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| author | Jian Sun Jinjin Yu Xinyi Niu Xinya Zhang Lili Zhou Xinyao Liu Bin Zhang Kun He Xiaofeng Niu Kin-Fai Ho Junji Cao Zhenxing Shen |
| author_facet | Jian Sun Jinjin Yu Xinyi Niu Xinya Zhang Lili Zhou Xinyao Liu Bin Zhang Kun He Xiaofeng Niu Kin-Fai Ho Junji Cao Zhenxing Shen |
| author_sort | Jian Sun |
| collection | DOAJ |
| description | PM2.5 (particulate matter with aerodynamic diameter ≤ 2.5 μm) is a well-known cytotoxic pollutant that capable to induce severe intracellular oxidative stress while the underlying mechanisms remain unclear. Herein, 4 types of PM2.5 derived from solid fuel burning were selected as stimuli in A549 cells exposure model to evaluate their effects on oxidative stress and inflammatory responses. Although resulting in different responses in cell viability, all PM2.5 exhibited over 50 % higher oxidative stress than control group, expression as intracellular reactive oxygen species, malondialdehyde and superoxide dismutase levels. The Pearson’s correlation results indicated that cations (e.g., Ca2+), heavy metals (e.g., Cr and Pb), nPAHs (nitro-polycyclic aromatic hydrocarbons, e.g., 6-nitrochrysene) and oPAHs (oxygenated PAHs, e.g., 9-fluorenone) were the main functioning toxics (r > 0.6). A key finding was the dual-directional regulation function of ECG (epicatechin gallate), that is, it could either increase the low A549 cell viabilities in coal combustion PM2.5 group or reduce them in charcoal PM2.5 group (P < 0.05). The dual-directional effects were likely because ECG can activate Nrf2 oxidation signaling pathway then inhibit the inflammatory signaling pathway NF-κB accordingly. Therefore, evidences indicated cytotoxicity of solid fuel derived PM2.5 were mainly caused by oxidative stress, which was proved to be reversed by green tea, providing a potential therapy method to PM2.5 and other hazards. |
| first_indexed | 2024-04-10T21:41:09Z |
| format | Article |
| id | doaj.art-29704e1c3c90486f905278d5c07dfeb6 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-04-10T21:41:09Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-29704e1c3c90486f905278d5c07dfeb62023-01-19T04:16:10ZengElsevierEnvironment International0160-41202023-01-01171107674Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green teaJian Sun0Jinjin Yu1Xinyi Niu2Xinya Zhang3Lili Zhou4Xinyao Liu5Bin Zhang6Kun He7Xiaofeng Niu8Kin-Fai Ho9Junji Cao10Zhenxing Shen11Department of Environmental Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaDepartment of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710049, ChinaSchool of Human Settlements and Civil Engineering, Xi'an Jiaotong University, Xi'an 710049, ChinaDepartment of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710049, ChinaDepartment of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710049, ChinaDepartment of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710049, ChinaDepartment of Environmental Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaDepartment of Environmental Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaDepartment of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710049, China; Corresponding authors.The Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, ChinaKey Lab of Aerosol Chemistry & Physics, SKLLQG, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an, ChinaDepartment of Environmental Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, China; Corresponding authors.PM2.5 (particulate matter with aerodynamic diameter ≤ 2.5 μm) is a well-known cytotoxic pollutant that capable to induce severe intracellular oxidative stress while the underlying mechanisms remain unclear. Herein, 4 types of PM2.5 derived from solid fuel burning were selected as stimuli in A549 cells exposure model to evaluate their effects on oxidative stress and inflammatory responses. Although resulting in different responses in cell viability, all PM2.5 exhibited over 50 % higher oxidative stress than control group, expression as intracellular reactive oxygen species, malondialdehyde and superoxide dismutase levels. The Pearson’s correlation results indicated that cations (e.g., Ca2+), heavy metals (e.g., Cr and Pb), nPAHs (nitro-polycyclic aromatic hydrocarbons, e.g., 6-nitrochrysene) and oPAHs (oxygenated PAHs, e.g., 9-fluorenone) were the main functioning toxics (r > 0.6). A key finding was the dual-directional regulation function of ECG (epicatechin gallate), that is, it could either increase the low A549 cell viabilities in coal combustion PM2.5 group or reduce them in charcoal PM2.5 group (P < 0.05). The dual-directional effects were likely because ECG can activate Nrf2 oxidation signaling pathway then inhibit the inflammatory signaling pathway NF-κB accordingly. Therefore, evidences indicated cytotoxicity of solid fuel derived PM2.5 were mainly caused by oxidative stress, which was proved to be reversed by green tea, providing a potential therapy method to PM2.5 and other hazards.http://www.sciencedirect.com/science/article/pii/S0160412022006018Epicatechin gallatePM2.5CytotoxicityDetoxification effectAntioxidant |
| spellingShingle | Jian Sun Jinjin Yu Xinyi Niu Xinya Zhang Lili Zhou Xinyao Liu Bin Zhang Kun He Xiaofeng Niu Kin-Fai Ho Junji Cao Zhenxing Shen Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea Environment International Epicatechin gallate PM2.5 Cytotoxicity Detoxification effect Antioxidant |
| title | Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea |
| title_full | Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea |
| title_fullStr | Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea |
| title_full_unstemmed | Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea |
| title_short | Solid fuel derived PM2.5 induced oxidative stress and according cytotoxicity in A549 cells: The evidence and potential neutralization by green tea |
| title_sort | solid fuel derived pm2 5 induced oxidative stress and according cytotoxicity in a549 cells the evidence and potential neutralization by green tea |
| topic | Epicatechin gallate PM2.5 Cytotoxicity Detoxification effect Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S0160412022006018 |
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