Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure
Antibiotic contamination of the environment negatively affects soil fertility by disrupting natural microbial communities. Currently, the study of the effect of antibacterial drugs on soils typical in Russia, which are of great importance for agriculture, is insufficient. Despite a rapid increase in...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/8/970 |
| _version_ | 1797524824659066880 |
|---|---|
| author | Tatiana Trifonova Anastasia Kosmacheva Alexander Sprygin Svetlana Chesnokova Olga Byadovskaya |
| author_facet | Tatiana Trifonova Anastasia Kosmacheva Alexander Sprygin Svetlana Chesnokova Olga Byadovskaya |
| author_sort | Tatiana Trifonova |
| collection | DOAJ |
| description | Antibiotic contamination of the environment negatively affects soil fertility by disrupting natural microbial communities. Currently, the study of the effect of antibacterial drugs on soils typical in Russia, which are of great importance for agriculture, is insufficient. Despite a rapid increase in the number of metagenomic studies, this article is the first publication devoted to the microbial diversity of sod-podzolic soil and its relationship with enzymatic activity. In the present study, we use 16S rRNA metagenomic sequencing to analyze microbiota dynamics and to examine soil enzymatic activities after antibiotic treatment with benzylpenicillin, oxytetracycline, and tylosin. We found that, following treatment, urease activity was reduced regardless of the antibiotic used while nitrification activity showed no statistically significant changes (<i>p</i> > 0.05). Oxytetracycline and tylosin produced no effect on catalase activity but benzylpenicillin caused an increase. Benzylpenicillin and oxytetracycline increased cellulolytic activity whereas tylosin had no significant effect (<i>p</i> > 0.05). Microbiome profiling through 16S rRNA gene sequencing demonstrated antibiotic administration and exhibited no significant impact on bacterial abundance and species diversity (<i>p</i> > 0.05), thus pointing to the resilience of the soil microbial community. Oxytetracycline, benzylpenicillin, and tylosin are likely to negatively affect the enzymatic profiles in sod-podzolic soil but with a negligible influence on the bacterial composition. |
| first_indexed | 2024-03-10T09:03:04Z |
| format | Article |
| id | doaj.art-2970fd00308d4ff1a77627006f04b381 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:03:04Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-2970fd00308d4ff1a77627006f04b3812023-11-22T06:34:02ZengMDPI AGAntibiotics2079-63822021-08-0110897010.3390/antibiotics10080970Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic ExposureTatiana Trifonova0Anastasia Kosmacheva1Alexander Sprygin2Svetlana Chesnokova3Olga Byadovskaya4Department of Soil Geography, M.V. Lomonosov Moscow State University, Moscow 119991, RussiaDepartment of Biology and Ecology, Vladimir State University Named after Alexander and Nikolay Stoletovs, Vladimir 600000, RussiaFGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”), Vladimir 600901, RussiaDepartment of Biology and Ecology, Vladimir State University Named after Alexander and Nikolay Stoletovs, Vladimir 600000, RussiaFGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”), Vladimir 600901, RussiaAntibiotic contamination of the environment negatively affects soil fertility by disrupting natural microbial communities. Currently, the study of the effect of antibacterial drugs on soils typical in Russia, which are of great importance for agriculture, is insufficient. Despite a rapid increase in the number of metagenomic studies, this article is the first publication devoted to the microbial diversity of sod-podzolic soil and its relationship with enzymatic activity. In the present study, we use 16S rRNA metagenomic sequencing to analyze microbiota dynamics and to examine soil enzymatic activities after antibiotic treatment with benzylpenicillin, oxytetracycline, and tylosin. We found that, following treatment, urease activity was reduced regardless of the antibiotic used while nitrification activity showed no statistically significant changes (<i>p</i> > 0.05). Oxytetracycline and tylosin produced no effect on catalase activity but benzylpenicillin caused an increase. Benzylpenicillin and oxytetracycline increased cellulolytic activity whereas tylosin had no significant effect (<i>p</i> > 0.05). Microbiome profiling through 16S rRNA gene sequencing demonstrated antibiotic administration and exhibited no significant impact on bacterial abundance and species diversity (<i>p</i> > 0.05), thus pointing to the resilience of the soil microbial community. Oxytetracycline, benzylpenicillin, and tylosin are likely to negatively affect the enzymatic profiles in sod-podzolic soil but with a negligible influence on the bacterial composition.https://www.mdpi.com/2079-6382/10/8/970benzylpenicillinoxytetracyclinetylosinmicrobiomebacterial community |
| spellingShingle | Tatiana Trifonova Anastasia Kosmacheva Alexander Sprygin Svetlana Chesnokova Olga Byadovskaya Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure Antibiotics benzylpenicillin oxytetracycline tylosin microbiome bacterial community |
| title | Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure |
| title_full | Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure |
| title_fullStr | Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure |
| title_full_unstemmed | Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure |
| title_short | Enzymatic Activity and Microbial Diversity of Sod-Podzolic Soil Microbiota Using 16S rRNA Amplicon Sequencing following Antibiotic Exposure |
| title_sort | enzymatic activity and microbial diversity of sod podzolic soil microbiota using 16s rrna amplicon sequencing following antibiotic exposure |
| topic | benzylpenicillin oxytetracycline tylosin microbiome bacterial community |
| url | https://www.mdpi.com/2079-6382/10/8/970 |
| work_keys_str_mv | AT tatianatrifonova enzymaticactivityandmicrobialdiversityofsodpodzolicsoilmicrobiotausing16srrnaampliconsequencingfollowingantibioticexposure AT anastasiakosmacheva enzymaticactivityandmicrobialdiversityofsodpodzolicsoilmicrobiotausing16srrnaampliconsequencingfollowingantibioticexposure AT alexandersprygin enzymaticactivityandmicrobialdiversityofsodpodzolicsoilmicrobiotausing16srrnaampliconsequencingfollowingantibioticexposure AT svetlanachesnokova enzymaticactivityandmicrobialdiversityofsodpodzolicsoilmicrobiotausing16srrnaampliconsequencingfollowingantibioticexposure AT olgabyadovskaya enzymaticactivityandmicrobialdiversityofsodpodzolicsoilmicrobiotausing16srrnaampliconsequencingfollowingantibioticexposure |