Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line
In this study, we investigated the beneficial effects of grapefruit IntegroPectin, derived from industrial waste grapefruit peels via hydrodynamic cavitation, on microglia cells exposed to oxidative stress conditions. Grapefruit IntegroPectin fully counteracted cell death and the apoptotic process i...
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MDPI AG
2024-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/4/355 |
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| author | Miriana Scordino Giulia Urone Monica Frinchi Chiara Valenza Angela Bonura Chiara Cipollina Rosaria Ciriminna Francesco Meneguzzo Mario Pagliaro Giuseppa Mudò Valentina Di Liberto |
| author_facet | Miriana Scordino Giulia Urone Monica Frinchi Chiara Valenza Angela Bonura Chiara Cipollina Rosaria Ciriminna Francesco Meneguzzo Mario Pagliaro Giuseppa Mudò Valentina Di Liberto |
| author_sort | Miriana Scordino |
| collection | DOAJ |
| description | In this study, we investigated the beneficial effects of grapefruit IntegroPectin, derived from industrial waste grapefruit peels via hydrodynamic cavitation, on microglia cells exposed to oxidative stress conditions. Grapefruit IntegroPectin fully counteracted cell death and the apoptotic process induced by cell exposure to tert-butyl hydroperoxide (TBH), a powerful hydroperoxide. The protective effects of the grapefruit IntegroPectin were accompanied with a decrease in the amount of ROS, and were strictly dependent on the activation of the phosphoinositide 3-kinase (PI3K)/Akt cascade. Finally, IntegroPectin treatment inhibited the neuroinflammatory response and the basal microglia activation by down-regulating the PI3K- nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)- inducible nitric oxide synthase (iNOS) cascade. These data strongly support further investigations aimed at exploring IntegroPectin’s therapeutic role in in vivo models of neurodegenerative disorders, characterized by a combination of chronic neurodegeneration, oxidative stress and neuroinflammation. |
| first_indexed | 2024-03-07T22:37:24Z |
| format | Article |
| id | doaj.art-297475a5855a4aa2903a100a59aad8fe |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-07T22:37:24Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-297475a5855a4aa2903a100a59aad8fe2024-02-23T15:11:59ZengMDPI AGCells2073-44092024-02-0113435510.3390/cells13040355Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell LineMiriana Scordino0Giulia Urone1Monica Frinchi2Chiara Valenza3Angela Bonura4Chiara Cipollina5Rosaria Ciriminna6Francesco Meneguzzo7Mario Pagliaro8Giuseppa Mudò9Valentina Di Liberto10Dipartimento di Biomedicina, Neuroscienze e Diagnostica Avanzata, Università di Palermo, Corso Tukory 129, 90134 Palermo, ItalyDipartimento di Biomedicina, Neuroscienze e Diagnostica Avanzata, Università di Palermo, Corso Tukory 129, 90134 Palermo, ItalyDipartimento di Biomedicina, Neuroscienze e Diagnostica Avanzata, Università di Palermo, Corso Tukory 129, 90134 Palermo, ItalyDipartimento di Biomedicina, Neuroscienze e Diagnostica Avanzata, Università di Palermo, Corso Tukory 129, 90134 Palermo, ItalyIstituto di Farmacologia Traslazionale, CNR, Via U. La Malfa 153, 90146 Palermo, ItalyFondazione RiMED, Via Ugo La Malfa 153, 90146 Palermo, ItalyIstituto per lo Studio dei Materiali Nanostrutturati, CNR, Via U. La Malfa 153, 90146 Palermo, ItalyIstituto per la Bioeconomia, CNR, Via Madonna del Piano 10, Sesto Fiorentino, 50019 Florence, ItalyIstituto per lo Studio dei Materiali Nanostrutturati, CNR, Via U. La Malfa 153, 90146 Palermo, ItalyDipartimento di Biomedicina, Neuroscienze e Diagnostica Avanzata, Università di Palermo, Corso Tukory 129, 90134 Palermo, ItalyDipartimento di Biomedicina, Neuroscienze e Diagnostica Avanzata, Università di Palermo, Corso Tukory 129, 90134 Palermo, ItalyIn this study, we investigated the beneficial effects of grapefruit IntegroPectin, derived from industrial waste grapefruit peels via hydrodynamic cavitation, on microglia cells exposed to oxidative stress conditions. Grapefruit IntegroPectin fully counteracted cell death and the apoptotic process induced by cell exposure to tert-butyl hydroperoxide (TBH), a powerful hydroperoxide. The protective effects of the grapefruit IntegroPectin were accompanied with a decrease in the amount of ROS, and were strictly dependent on the activation of the phosphoinositide 3-kinase (PI3K)/Akt cascade. Finally, IntegroPectin treatment inhibited the neuroinflammatory response and the basal microglia activation by down-regulating the PI3K- nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)- inducible nitric oxide synthase (iNOS) cascade. These data strongly support further investigations aimed at exploring IntegroPectin’s therapeutic role in in vivo models of neurodegenerative disorders, characterized by a combination of chronic neurodegeneration, oxidative stress and neuroinflammation.https://www.mdpi.com/2073-4409/13/4/355pectinmicrogliaoxidative stressneuroinflammationAktERK |
| spellingShingle | Miriana Scordino Giulia Urone Monica Frinchi Chiara Valenza Angela Bonura Chiara Cipollina Rosaria Ciriminna Francesco Meneguzzo Mario Pagliaro Giuseppa Mudò Valentina Di Liberto Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line Cells pectin microglia oxidative stress neuroinflammation Akt ERK |
| title | Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line |
| title_full | Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line |
| title_fullStr | Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line |
| title_full_unstemmed | Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line |
| title_short | Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line |
| title_sort | anti apoptotic and anti inflammatory properties of grapefruit integropectin on human microglial hmc3 cell line |
| topic | pectin microglia oxidative stress neuroinflammation Akt ERK |
| url | https://www.mdpi.com/2073-4409/13/4/355 |
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