Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots
Background: <i>Brassica napus</i> is one of the most important oil crops in the world, and <i>B. napus</i> shoots are nutrient-rich fresh vegetables. The crude fiber (CF) component is one of the most important factors affecting the taste quality of <i>B. napus</i>...
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/2/403 |
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| author | Rui Shi Chengke Pang Xu Wu Xiaozhen Zhao Feng Chen Wei Zhang Chengming Sun Sanxiong Fu Maolong Hu Jiefu Zhang Xiaodong Wang |
| author_facet | Rui Shi Chengke Pang Xu Wu Xiaozhen Zhao Feng Chen Wei Zhang Chengming Sun Sanxiong Fu Maolong Hu Jiefu Zhang Xiaodong Wang |
| author_sort | Rui Shi |
| collection | DOAJ |
| description | Background: <i>Brassica napus</i> is one of the most important oil crops in the world, and <i>B. napus</i> shoots are nutrient-rich fresh vegetables. The crude fiber (CF) component is one of the most important factors affecting the taste quality of <i>B. napus</i> shoots, but the factors underlying the desirable low-CF trait remain poorly understood. Methods: In this study, a high-density single-nucleotide polymorphism (SNP) map was used to map quantitative trait loci (QTLs) for five CF-related traits in a recombinant inbred population. Results: A total of 49 QTLs were obtained in four environments, including eleven, twelve, eight, twelve and six QTLs for content of neutral detergent fiber, acid detergent fiber, acid detergent lignin, hemicellulose and cellulose, respectively. The phenotypic variation explained by single QTL ranged from 4.62% to 14.76%. Eight of these QTLs were further integrated into four unique QTLs, which controlled two different traits simultaneously. Five CF-component-related candidate genes were identified, among which <i>BnaC03g07110D</i> and <i>BnaC07g21271D</i> were considered to be the most likely candidate genes. In addition, five lines with low CF content were selected, which can be used as excellent germplasm resources in breeding. Conclusions: The QTLs identified in this study will contribute to our understanding of the genetic mechanism of CF and can be used as targets for reducing CF content in <i>B. napus</i> shoots. In addition, this study also provided excellent germplasm resources for low CF content breeding. |
| first_indexed | 2024-03-09T12:41:38Z |
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| language | English |
| last_indexed | 2024-03-09T12:41:38Z |
| publishDate | 2023-01-01 |
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| series | Foods |
| spelling | doaj.art-2976a93086de4eb8907520ee8b1815e02023-11-30T22:17:02ZengMDPI AGFoods2304-81582023-01-0112240310.3390/foods12020403Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. ShootsRui Shi0Chengke Pang1Xu Wu2Xiaozhen Zhao3Feng Chen4Wei Zhang5Chengming Sun6Sanxiong Fu7Maolong Hu8Jiefu Zhang9Xiaodong Wang10State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing 210014, ChinaBackground: <i>Brassica napus</i> is one of the most important oil crops in the world, and <i>B. napus</i> shoots are nutrient-rich fresh vegetables. The crude fiber (CF) component is one of the most important factors affecting the taste quality of <i>B. napus</i> shoots, but the factors underlying the desirable low-CF trait remain poorly understood. Methods: In this study, a high-density single-nucleotide polymorphism (SNP) map was used to map quantitative trait loci (QTLs) for five CF-related traits in a recombinant inbred population. Results: A total of 49 QTLs were obtained in four environments, including eleven, twelve, eight, twelve and six QTLs for content of neutral detergent fiber, acid detergent fiber, acid detergent lignin, hemicellulose and cellulose, respectively. The phenotypic variation explained by single QTL ranged from 4.62% to 14.76%. Eight of these QTLs were further integrated into four unique QTLs, which controlled two different traits simultaneously. Five CF-component-related candidate genes were identified, among which <i>BnaC03g07110D</i> and <i>BnaC07g21271D</i> were considered to be the most likely candidate genes. In addition, five lines with low CF content were selected, which can be used as excellent germplasm resources in breeding. Conclusions: The QTLs identified in this study will contribute to our understanding of the genetic mechanism of CF and can be used as targets for reducing CF content in <i>B. napus</i> shoots. In addition, this study also provided excellent germplasm resources for low CF content breeding.https://www.mdpi.com/2304-8158/12/2/403<i>Brassica napus</i>crude fibergermplasm selectionQTL mappingshoots |
| spellingShingle | Rui Shi Chengke Pang Xu Wu Xiaozhen Zhao Feng Chen Wei Zhang Chengming Sun Sanxiong Fu Maolong Hu Jiefu Zhang Xiaodong Wang Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots Foods <i>Brassica napus</i> crude fiber germplasm selection QTL mapping shoots |
| title | Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots |
| title_full | Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots |
| title_fullStr | Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots |
| title_full_unstemmed | Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots |
| title_short | Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component in <i>Brassica napus</i> L. Shoots |
| title_sort | genetic dissection and germplasm selection of the low crude fiber component in i brassica napus i l shoots |
| topic | <i>Brassica napus</i> crude fiber germplasm selection QTL mapping shoots |
| url | https://www.mdpi.com/2304-8158/12/2/403 |
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