Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen
Here, Mac Kain and Maarifi et al. perform a functional CRISPR/Cas9 screen to identify SARS-CoV-2 restriction factors in A549 cells. They identify DAXX, a scaffold protein of nuclear bodies with diverse functions, that has anti-viral activity post SARS-CoV-2 entry, while SARS-CoV-2 has evolved a mech...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-30134-9 |
| _version_ | 1818194555987558400 |
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| author | Alice Mac Kain Ghizlane Maarifi Sophie-Marie Aicher Nathalie Arhel Artem Baidaliuk Sandie Munier Flora Donati Thomas Vallet Quang Dinh Tran Alexandra Hardy Maxime Chazal Françoise Porrot Molly OhAinle Jared Carlson-Stevermer Jennifer Oki Kevin Holden Gert Zimmer Etienne Simon-Lorière Timothée Bruel Olivier Schwartz Sylvie van der Werf Nolwenn Jouvenet Sébastien Nisole Marco Vignuzzi Ferdinand Roesch |
| author_facet | Alice Mac Kain Ghizlane Maarifi Sophie-Marie Aicher Nathalie Arhel Artem Baidaliuk Sandie Munier Flora Donati Thomas Vallet Quang Dinh Tran Alexandra Hardy Maxime Chazal Françoise Porrot Molly OhAinle Jared Carlson-Stevermer Jennifer Oki Kevin Holden Gert Zimmer Etienne Simon-Lorière Timothée Bruel Olivier Schwartz Sylvie van der Werf Nolwenn Jouvenet Sébastien Nisole Marco Vignuzzi Ferdinand Roesch |
| author_sort | Alice Mac Kain |
| collection | DOAJ |
| description | Here, Mac Kain and Maarifi et al. perform a functional CRISPR/Cas9 screen to identify SARS-CoV-2 restriction factors in A549 cells. They identify DAXX, a scaffold protein of nuclear bodies with diverse functions, that has anti-viral activity post SARS-CoV-2 entry, while SARS-CoV-2 has evolved a mechanism to counteract its action via PLpro-mediated proteasomal degradation. |
| first_indexed | 2024-12-12T01:04:10Z |
| format | Article |
| id | doaj.art-2979f6b8098645b5aa9271d3426c5eed |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-12T01:04:10Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-2979f6b8098645b5aa9271d3426c5eed2022-12-22T00:43:38ZengNature PortfolioNature Communications2041-17232022-05-0113111310.1038/s41467-022-30134-9Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screenAlice Mac Kain0Ghizlane Maarifi1Sophie-Marie Aicher2Nathalie Arhel3Artem Baidaliuk4Sandie Munier5Flora Donati6Thomas Vallet7Quang Dinh Tran8Alexandra Hardy9Maxime Chazal10Françoise Porrot11Molly OhAinle12Jared Carlson-Stevermer13Jennifer Oki14Kevin Holden15Gert Zimmer16Etienne Simon-Lorière17Timothée Bruel18Olivier Schwartz19Sylvie van der Werf20Nolwenn Jouvenet21Sébastien Nisole22Marco Vignuzzi23Ferdinand Roesch24Institut Pasteur, Université de Paris CitéInstitut de Recherche en Infectiologie de Montpellier (IRIM), , Université de Montpellier, CNRSInstitut Pasteur, Université de Paris CitéInstitut de Recherche en Infectiologie de Montpellier (IRIM), , Université de Montpellier, CNRSInstitut Pasteur, G5 Evolutionary genomics of RNA virusesInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéDivisions of Human Biology, Fred Hutchinson Cancer Research CenterSynthego CorporationSynthego CorporationSynthego CorporationInstitute of Virology and Immunology, Bern & Mittelhäusern, Switzerland, and Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of BernInstitut Pasteur, G5 Evolutionary genomics of RNA virusesInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéInstitut de Recherche en Infectiologie de Montpellier (IRIM), , Université de Montpellier, CNRSInstitut Pasteur, Université de Paris CitéInstitut Pasteur, Université de Paris CitéHere, Mac Kain and Maarifi et al. perform a functional CRISPR/Cas9 screen to identify SARS-CoV-2 restriction factors in A549 cells. They identify DAXX, a scaffold protein of nuclear bodies with diverse functions, that has anti-viral activity post SARS-CoV-2 entry, while SARS-CoV-2 has evolved a mechanism to counteract its action via PLpro-mediated proteasomal degradation.https://doi.org/10.1038/s41467-022-30134-9 |
| spellingShingle | Alice Mac Kain Ghizlane Maarifi Sophie-Marie Aicher Nathalie Arhel Artem Baidaliuk Sandie Munier Flora Donati Thomas Vallet Quang Dinh Tran Alexandra Hardy Maxime Chazal Françoise Porrot Molly OhAinle Jared Carlson-Stevermer Jennifer Oki Kevin Holden Gert Zimmer Etienne Simon-Lorière Timothée Bruel Olivier Schwartz Sylvie van der Werf Nolwenn Jouvenet Sébastien Nisole Marco Vignuzzi Ferdinand Roesch Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen Nature Communications |
| title | Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen |
| title_full | Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen |
| title_fullStr | Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen |
| title_full_unstemmed | Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen |
| title_short | Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen |
| title_sort | identification of daxx as a restriction factor of sars cov 2 through a crispr cas9 screen |
| url | https://doi.org/10.1038/s41467-022-30134-9 |
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