Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells
In this study, the soluble, but non-digestible, longan (<i>Dimocarpus longan Lour.</i>) polysaccharides (LP) were extracted from dried longan fruits and then chemically selenylated to produce two selenylated products, namely SeLP1 and SeLP2, with different selenylation extents. The aim w...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2072-6643/15/21/4679 |
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| author | Ya-Hui Yu Xin-Huai Zhao |
| author_facet | Ya-Hui Yu Xin-Huai Zhao |
| author_sort | Ya-Hui Yu |
| collection | DOAJ |
| description | In this study, the soluble, but non-digestible, longan (<i>Dimocarpus longan Lour.</i>) polysaccharides (LP) were extracted from dried longan fruits and then chemically selenylated to produce two selenylated products, namely SeLP1 and SeLP2, with different selenylation extents. The aim was to investigate their protective effects on rat intestinal epithelial (IEC-6) cells exposed to the food toxin fumonisin B1 (FB1). LP only contained total Se content of less than 0.01 g/kg, while SeLP1 and SeLP2 were measured with respective total Se content of up to 1.46 and 4.79 g/kg. The cell viability results showed that these two selenylated products were more efficient than LP in the IEC-6 cells in alleviating FB1-induced cell toxicity, suppressing lactate dehydrogenase (LDH) release, and decreasing the generation of intracellular reactive oxygen species (ROS). These two selenylated products were also more effective than LP in combating FB1-induced barrier disruption via increasing the transepithelial electric resistance (TEER), reducing the paracellular permeability, decreasing the mitochondrial membrane potential (MMP) loss, and maintaining cell barrier integrity by upregulating the tight-junction-related genes and proteins. FB1 caused cell oxidative stress and barrier dysfunction by activating the MAPK and mitochondrial apoptosis signaling pathways, while SeLP1 and SeLP2 could regulate the tMAPK- and apoptosis-related proteins to suppress the FB1-mediated activation of the two pathways. Overall, SeLP2 was observed to be more active than SeLP1 in the IEC-6 cells. In conclusion, the chemical selenylation of LP caused an activity enhancement to ameliorate the FB1-induced cell cytotoxicity and intestinal barrier disruption. Meanwhile, the increased selenylation of LP would endow the selenylated product SeLP2 with more activity. |
| first_indexed | 2024-03-11T11:23:09Z |
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| language | English |
| last_indexed | 2024-03-11T11:23:09Z |
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| series | Nutrients |
| spelling | doaj.art-297ac64331824079ae18785ca469bb532023-11-10T15:10:01ZengMDPI AGNutrients2072-66432023-11-011521467910.3390/nu15214679Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) CellsYa-Hui Yu0Xin-Huai Zhao1School of Biology and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, ChinaIn this study, the soluble, but non-digestible, longan (<i>Dimocarpus longan Lour.</i>) polysaccharides (LP) were extracted from dried longan fruits and then chemically selenylated to produce two selenylated products, namely SeLP1 and SeLP2, with different selenylation extents. The aim was to investigate their protective effects on rat intestinal epithelial (IEC-6) cells exposed to the food toxin fumonisin B1 (FB1). LP only contained total Se content of less than 0.01 g/kg, while SeLP1 and SeLP2 were measured with respective total Se content of up to 1.46 and 4.79 g/kg. The cell viability results showed that these two selenylated products were more efficient than LP in the IEC-6 cells in alleviating FB1-induced cell toxicity, suppressing lactate dehydrogenase (LDH) release, and decreasing the generation of intracellular reactive oxygen species (ROS). These two selenylated products were also more effective than LP in combating FB1-induced barrier disruption via increasing the transepithelial electric resistance (TEER), reducing the paracellular permeability, decreasing the mitochondrial membrane potential (MMP) loss, and maintaining cell barrier integrity by upregulating the tight-junction-related genes and proteins. FB1 caused cell oxidative stress and barrier dysfunction by activating the MAPK and mitochondrial apoptosis signaling pathways, while SeLP1 and SeLP2 could regulate the tMAPK- and apoptosis-related proteins to suppress the FB1-mediated activation of the two pathways. Overall, SeLP2 was observed to be more active than SeLP1 in the IEC-6 cells. In conclusion, the chemical selenylation of LP caused an activity enhancement to ameliorate the FB1-induced cell cytotoxicity and intestinal barrier disruption. Meanwhile, the increased selenylation of LP would endow the selenylated product SeLP2 with more activity.https://www.mdpi.com/2072-6643/15/21/4679longan polysaccharidesselenylationfumonisin B1IEC-6 cellscytotoxicitybarrier function |
| spellingShingle | Ya-Hui Yu Xin-Huai Zhao Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells Nutrients longan polysaccharides selenylation fumonisin B1 IEC-6 cells cytotoxicity barrier function |
| title | Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells |
| title_full | Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells |
| title_fullStr | Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells |
| title_full_unstemmed | Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells |
| title_short | Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells |
| title_sort | longan polysaccharides with covalent selenylation combat the fumonisin b1 induced cell toxicity and barrier disruption in intestinal epithelial iec 6 cells |
| topic | longan polysaccharides selenylation fumonisin B1 IEC-6 cells cytotoxicity barrier function |
| url | https://www.mdpi.com/2072-6643/15/21/4679 |
| work_keys_str_mv | AT yahuiyu longanpolysaccharideswithcovalentselenylationcombatthefumonisinb1inducedcelltoxicityandbarrierdisruptioninintestinalepithelialiec6cells AT xinhuaizhao longanpolysaccharideswithcovalentselenylationcombatthefumonisinb1inducedcelltoxicityandbarrierdisruptioninintestinalepithelialiec6cells |