The Role of PPK26 in Drosophila Larval Mechanical Nociception
In Drosophila larvae, the class IV dendritic arborization (da) neurons are polymodal nociceptors. Here, we show that ppk26 (CG8546) plays an important role in mechanical nociception in class IV da neurons. Our immunohistochemical and functional results demonstrate that ppk26 is specifically expresse...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714008742 |
| _version_ | 1819078240423116800 |
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| author | Yanmeng Guo Yuping Wang Qingxiu Wang Zuoren Wang |
| author_facet | Yanmeng Guo Yuping Wang Qingxiu Wang Zuoren Wang |
| author_sort | Yanmeng Guo |
| collection | DOAJ |
| description | In Drosophila larvae, the class IV dendritic arborization (da) neurons are polymodal nociceptors. Here, we show that ppk26 (CG8546) plays an important role in mechanical nociception in class IV da neurons. Our immunohistochemical and functional results demonstrate that ppk26 is specifically expressed in class IV da neurons. Larvae with mutant ppk26 showed severe behavioral defects in a mechanical nociception behavioral test but responded to noxious heat stimuli comparably to wild-type larvae. In addition, functional studies suggest that ppk26 and ppk (also called ppk1) function in the same pathway, whereas piezo functions in a parallel pathway. Consistent with these functional results, we found that PPK and PPK26 are interdependent on each other for their cell surface localization. Our work indicates that PPK26 and PPK might form heteromeric DEG/ENaC channels that are essential for mechanotransduction in class IV da neurons. |
| first_indexed | 2024-12-21T19:09:57Z |
| format | Article |
| id | doaj.art-297b4b3fcb6d4219baa2e0f9de2651a3 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T19:09:57Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-297b4b3fcb6d4219baa2e0f9de2651a32022-12-21T18:53:13ZengElsevierCell Reports2211-12472014-11-01941183119010.1016/j.celrep.2014.10.020The Role of PPK26 in Drosophila Larval Mechanical NociceptionYanmeng Guo0Yuping Wang1Qingxiu Wang2Zuoren Wang3Institute of Neuroscience, CAS Center for Excellence in Brain Science, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaInstitute of Neuroscience, CAS Center for Excellence in Brain Science, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaInstitute of Neuroscience, CAS Center for Excellence in Brain Science, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaInstitute of Neuroscience, CAS Center for Excellence in Brain Science, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaIn Drosophila larvae, the class IV dendritic arborization (da) neurons are polymodal nociceptors. Here, we show that ppk26 (CG8546) plays an important role in mechanical nociception in class IV da neurons. Our immunohistochemical and functional results demonstrate that ppk26 is specifically expressed in class IV da neurons. Larvae with mutant ppk26 showed severe behavioral defects in a mechanical nociception behavioral test but responded to noxious heat stimuli comparably to wild-type larvae. In addition, functional studies suggest that ppk26 and ppk (also called ppk1) function in the same pathway, whereas piezo functions in a parallel pathway. Consistent with these functional results, we found that PPK and PPK26 are interdependent on each other for their cell surface localization. Our work indicates that PPK26 and PPK might form heteromeric DEG/ENaC channels that are essential for mechanotransduction in class IV da neurons.http://www.sciencedirect.com/science/article/pii/S2211124714008742 |
| spellingShingle | Yanmeng Guo Yuping Wang Qingxiu Wang Zuoren Wang The Role of PPK26 in Drosophila Larval Mechanical Nociception Cell Reports |
| title | The Role of PPK26 in Drosophila Larval Mechanical Nociception |
| title_full | The Role of PPK26 in Drosophila Larval Mechanical Nociception |
| title_fullStr | The Role of PPK26 in Drosophila Larval Mechanical Nociception |
| title_full_unstemmed | The Role of PPK26 in Drosophila Larval Mechanical Nociception |
| title_short | The Role of PPK26 in Drosophila Larval Mechanical Nociception |
| title_sort | role of ppk26 in drosophila larval mechanical nociception |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714008742 |
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