Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a...
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2021-12-01
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author | Xiangchun Zhang Qinqin Zheng Ziqi Wang Chao Xu Haolei Han Aiping Li Guicen Ma Jiaojiao Li Chengyin Lu Hongping Chen Zhichao Zhang |
author_facet | Xiangchun Zhang Qinqin Zheng Ziqi Wang Chao Xu Haolei Han Aiping Li Guicen Ma Jiaojiao Li Chengyin Lu Hongping Chen Zhichao Zhang |
author_sort | Xiangchun Zhang |
collection | DOAJ |
description | Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk. |
first_indexed | 2024-03-10T03:28:44Z |
format | Article |
id | doaj.art-297bb936ab024ae180280bb4abf07c30 |
institution | Directory Open Access Journal |
issn | 2079-4991 |
language | English |
last_indexed | 2024-03-10T03:28:44Z |
publishDate | 2021-12-01 |
publisher | MDPI AG |
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series | Nanomaterials |
spelling | doaj.art-297bb936ab024ae180280bb4abf07c302023-11-23T12:02:08ZengMDPI AGNanomaterials2079-49912021-12-0112114510.3390/nano12010145Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au ClustersXiangchun Zhang0Qinqin Zheng1Ziqi Wang2Chao Xu3Haolei Han4Aiping Li5Guicen Ma6Jiaojiao Li7Chengyin Lu8Hongping Chen9Zhichao Zhang10Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaDepartment of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaDepartment of Musculoskeletal Tumor, Fudan University Shanghai Cancer Center, Shanghai 200032, ChinaTumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.https://www.mdpi.com/2079-4991/12/1/145Au clustersMT1-MMPinvasion/metastasishuman cervical cancer |
spellingShingle | Xiangchun Zhang Qinqin Zheng Ziqi Wang Chao Xu Haolei Han Aiping Li Guicen Ma Jiaojiao Li Chengyin Lu Hongping Chen Zhichao Zhang Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters Nanomaterials Au clusters MT1-MMP invasion/metastasis human cervical cancer |
title | Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters |
title_full | Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters |
title_fullStr | Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters |
title_full_unstemmed | Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters |
title_short | Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters |
title_sort | qualitative and quantitative analysis of tumor cell invasion using au clusters |
topic | Au clusters MT1-MMP invasion/metastasis human cervical cancer |
url | https://www.mdpi.com/2079-4991/12/1/145 |
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