Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters

Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a...

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Main Authors: Xiangchun Zhang, Qinqin Zheng, Ziqi Wang, Chao Xu, Haolei Han, Aiping Li, Guicen Ma, Jiaojiao Li, Chengyin Lu, Hongping Chen, Zhichao Zhang
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Nanomaterials
Subjects:
Online Access:https://www.mdpi.com/2079-4991/12/1/145
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author Xiangchun Zhang
Qinqin Zheng
Ziqi Wang
Chao Xu
Haolei Han
Aiping Li
Guicen Ma
Jiaojiao Li
Chengyin Lu
Hongping Chen
Zhichao Zhang
author_facet Xiangchun Zhang
Qinqin Zheng
Ziqi Wang
Chao Xu
Haolei Han
Aiping Li
Guicen Ma
Jiaojiao Li
Chengyin Lu
Hongping Chen
Zhichao Zhang
author_sort Xiangchun Zhang
collection DOAJ
description Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.
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spelling doaj.art-297bb936ab024ae180280bb4abf07c302023-11-23T12:02:08ZengMDPI AGNanomaterials2079-49912021-12-0112114510.3390/nano12010145Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au ClustersXiangchun Zhang0Qinqin Zheng1Ziqi Wang2Chao Xu3Haolei Han4Aiping Li5Guicen Ma6Jiaojiao Li7Chengyin Lu8Hongping Chen9Zhichao Zhang10Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaDepartment of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaDepartment of Musculoskeletal Tumor, Fudan University Shanghai Cancer Center, Shanghai 200032, ChinaTumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.https://www.mdpi.com/2079-4991/12/1/145Au clustersMT1-MMPinvasion/metastasishuman cervical cancer
spellingShingle Xiangchun Zhang
Qinqin Zheng
Ziqi Wang
Chao Xu
Haolei Han
Aiping Li
Guicen Ma
Jiaojiao Li
Chengyin Lu
Hongping Chen
Zhichao Zhang
Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
Nanomaterials
Au clusters
MT1-MMP
invasion/metastasis
human cervical cancer
title Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
title_full Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
title_fullStr Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
title_full_unstemmed Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
title_short Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters
title_sort qualitative and quantitative analysis of tumor cell invasion using au clusters
topic Au clusters
MT1-MMP
invasion/metastasis
human cervical cancer
url https://www.mdpi.com/2079-4991/12/1/145
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