Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis
Ornithobacterium rhinotracheale (ORT) has been identified as one of the respiratory bacterial pathogens in turkey and chicken flocks. Four live birds displaying severe torticollis were submitted from a 13-week-old commercial broiler breeder chicken flock located in Mazandaran province. These birds w...
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Razi Vaccine and Serum Research Institute
2015-10-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://www.archrazi.com/browse.php?a_id=583&sid=1&slc_lang=en |
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| author | Banani, M Hablolvarid, M.H Momayez, R Nouri, A Ghodsian, N Ashtari, A Mirzaei.S.G |
| author_facet | Banani, M Hablolvarid, M.H Momayez, R Nouri, A Ghodsian, N Ashtari, A Mirzaei.S.G |
| author_sort | Banani, M |
| collection | DOAJ |
| description | Ornithobacterium rhinotracheale (ORT) has been identified as one of the respiratory bacterial pathogens in turkey and chicken flocks. Four live birds displaying severe torticollis were submitted from a 13-week-old commercial broiler breeder chicken flock located in Mazandaran province. These birds were suspected to
pasteurellosis by the farm veterinarian. No other marked gross lesion except emaciation was seen. Histopathologic examination of the brains showed mild to moderate meningeal vasculitis, perivascular cuffing with lymphocytes, degeneration and necrosis of purkinje cells in the cerebellum. Viral culture of the brains
especially for Newcastle disease and avian influenza viruses was negative. Bacterial culture of the brains onto the blood agar revealed pure growth of Ornithobacterium rhinotracheale. In this study molecular confirmation of ORT by using of a very specific polymerase chain reaction (PCR) was carried out. Amplification products of a 784 bp region of the 16S rRNA gene of ORT confirmed the bacterium identification. This is the first field case
of ORT isolation from the brain of commercial chickens in Iran. These data suggest that this bacterium should be considered in differential diagnosis in cases of avian nervous signs. Further studies are necessary to confirm if ORT is a primary pathogen in such cases. |
| first_indexed | 2024-04-12T16:29:03Z |
| format | Article |
| id | doaj.art-297c5c9aeb4149aaa6cb0cf9cd99c052 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-12T16:29:03Z |
| publishDate | 2015-10-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-297c5c9aeb4149aaa6cb0cf9cd99c0522022-12-22T03:25:15ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722015-10-017003203209Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitisBanani, M0Hablolvarid, M.H1Momayez, R2Nouri, A3Ghodsian, N4Ashtari, A5Mirzaei.S.G6Department of Avian Bacterial Diseases Research & Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Pathology & Epidemiology Research & Diagnosis, Razi vaccine and Serum Research Institute, Karaj, IranDepartment of Avian Bacterial Diseases Research & Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Avian Bacterial Diseases Research & Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Quality Control, Razi vaccine and Serum Research Institute, Karaj, IranDepartment of Avian Bacterial Diseases Research & Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Avian Bacterial Diseases Research & Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, IranOrnithobacterium rhinotracheale (ORT) has been identified as one of the respiratory bacterial pathogens in turkey and chicken flocks. Four live birds displaying severe torticollis were submitted from a 13-week-old commercial broiler breeder chicken flock located in Mazandaran province. These birds were suspected to pasteurellosis by the farm veterinarian. No other marked gross lesion except emaciation was seen. Histopathologic examination of the brains showed mild to moderate meningeal vasculitis, perivascular cuffing with lymphocytes, degeneration and necrosis of purkinje cells in the cerebellum. Viral culture of the brains especially for Newcastle disease and avian influenza viruses was negative. Bacterial culture of the brains onto the blood agar revealed pure growth of Ornithobacterium rhinotracheale. In this study molecular confirmation of ORT by using of a very specific polymerase chain reaction (PCR) was carried out. Amplification products of a 784 bp region of the 16S rRNA gene of ORT confirmed the bacterium identification. This is the first field case of ORT isolation from the brain of commercial chickens in Iran. These data suggest that this bacterium should be considered in differential diagnosis in cases of avian nervous signs. Further studies are necessary to confirm if ORT is a primary pathogen in such cases.http://www.archrazi.com/browse.php?a_id=583&sid=1&slc_lang=enIsolationOrnithobacterium rhinotrachealebrainbroiler breederchickensIran |
| spellingShingle | Banani, M Hablolvarid, M.H Momayez, R Nouri, A Ghodsian, N Ashtari, A Mirzaei.S.G Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis Archives of Razi Institute Isolation Ornithobacterium rhinotracheale brain broiler breeder chickens Iran |
| title | Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis |
| title_full | Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis |
| title_fullStr | Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis |
| title_full_unstemmed | Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis |
| title_short | Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis |
| title_sort | isolation of ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis |
| topic | Isolation Ornithobacterium rhinotracheale brain broiler breeder chickens Iran |
| url | http://www.archrazi.com/browse.php?a_id=583&sid=1&slc_lang=en |
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