ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats
We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT therm...
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The Royal Society
2023-05-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.230109 |
| _version_ | 1797834639511912448 |
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| author | Marija Aleksic Igor Golic Aleksandra Jankovic Aleksandra Cvoro Aleksandra Korac |
| author_facet | Marija Aleksic Igor Golic Aleksandra Jankovic Aleksandra Cvoro Aleksandra Korac |
| author_sort | Marija Aleksic |
| collection | DOAJ |
| description | We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes. |
| first_indexed | 2024-04-09T14:40:56Z |
| format | Article |
| id | doaj.art-297e618ef21c4f60bc5c0c02ac13f221 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-09T14:40:56Z |
| publishDate | 2023-05-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-297e618ef21c4f60bc5c0c02ac13f2212023-05-03T07:05:24ZengThe Royal SocietyRoyal Society Open Science2054-57032023-05-0110510.1098/rsos.230109ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid ratsMarija Aleksic0Igor Golic1Aleksandra Jankovic2Aleksandra Cvoro3Aleksandra Korac4Center for Electron Microscopy, Faculty of Biology, University of Belgrade, Belgrade 11000, SerbiaCenter for Electron Microscopy, Faculty of Biology, University of Belgrade, Belgrade 11000, SerbiaInstitute for Biological Research 'Sinisa Stankovic'—National Institute of Republic of Serbia, University of Belgrade, Belgrade 11000, SerbiaCenter for Electron Microscopy, Faculty of Biology, University of Belgrade, Belgrade 11000, SerbiaCenter for Electron Microscopy, Faculty of Biology, University of Belgrade, Belgrade 11000, SerbiaWe previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.https://royalsocietypublishing.org/doi/10.1098/rsos.230109hypothyroidismbrown adipocytesperoxisomesACOX1ACOX3 |
| spellingShingle | Marija Aleksic Igor Golic Aleksandra Jankovic Aleksandra Cvoro Aleksandra Korac ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats Royal Society Open Science hypothyroidism brown adipocytes peroxisomes ACOX1 ACOX3 |
| title | ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats |
| title_full | ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats |
| title_fullStr | ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats |
| title_full_unstemmed | ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats |
| title_short | ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats |
| title_sort | acox driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats |
| topic | hypothyroidism brown adipocytes peroxisomes ACOX1 ACOX3 |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.230109 |
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