Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species
A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distr...
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| Format: | Article |
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MDPI AG
2019-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/9/2/60 |
| _version_ | 1818616130366865408 |
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| author | Tao Lang Guangrong Li Zhihui Yu Jiwei Ma Qiheng Chen Ennian Yang Zujun Yang |
| author_facet | Tao Lang Guangrong Li Zhihui Yu Jiwei Ma Qiheng Chen Ennian Yang Zujun Yang |
| author_sort | Tao Lang |
| collection | DOAJ |
| description | A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distribution of Ta-3A1 in chromosomes 3A, 5A, 5B, 5D, and 7A of wheat was confirmed by nondenaturing fluorescence in situ hybridization (ND-FISH) after labeling the oligonucleotide probe. The analysis of monomer variants indicated that rapid sequence amplification of Ta-3A1 occurred first on chromosomes of linkage group 5, then groups 3 and 7. Comparative ND-FISH analysis suggested that rapid changes occurred in copy number and chromosomal locations of Ta-3A1 among the different species in the tribe Triticeae, which may have been associated with chromosomal rearrangements during speciation and polyploidization. The labeling and subsequent use of Ta-3A1 by ND-FISH may assist in the precise identification and documentation of novel wheat germplasm engineered by chromosome manipulation. |
| first_indexed | 2024-12-16T16:44:54Z |
| format | Article |
| id | doaj.art-297fea67afae40a1b08d6c8fa6a3c87e |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-16T16:44:54Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj.art-297fea67afae40a1b08d6c8fa6a3c87e2022-12-21T22:24:13ZengMDPI AGAgronomy2073-43952019-01-01926010.3390/agronomy9020060agronomy9020060Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related SpeciesTao Lang0Guangrong Li1Zhihui Yu2Jiwei Ma3Qiheng Chen4Ennian Yang5Zujun Yang6Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaCenter for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaCenter for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaCenter for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaCenter for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCenter for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaA large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distribution of Ta-3A1 in chromosomes 3A, 5A, 5B, 5D, and 7A of wheat was confirmed by nondenaturing fluorescence in situ hybridization (ND-FISH) after labeling the oligonucleotide probe. The analysis of monomer variants indicated that rapid sequence amplification of Ta-3A1 occurred first on chromosomes of linkage group 5, then groups 3 and 7. Comparative ND-FISH analysis suggested that rapid changes occurred in copy number and chromosomal locations of Ta-3A1 among the different species in the tribe Triticeae, which may have been associated with chromosomal rearrangements during speciation and polyploidization. The labeling and subsequent use of Ta-3A1 by ND-FISH may assist in the precise identification and documentation of novel wheat germplasm engineered by chromosome manipulation.https://www.mdpi.com/2073-4395/9/2/60fluorescence in situ hybridizationmini-satellitetandem repeatswheat |
| spellingShingle | Tao Lang Guangrong Li Zhihui Yu Jiwei Ma Qiheng Chen Ennian Yang Zujun Yang Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species Agronomy fluorescence in situ hybridization mini-satellite tandem repeats wheat |
| title | Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species |
| title_full | Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species |
| title_fullStr | Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species |
| title_full_unstemmed | Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species |
| title_short | Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species |
| title_sort | genome wide distribution of novel ta 3a1 mini satellite repeats and its use for chromosome identification in wheat and related species |
| topic | fluorescence in situ hybridization mini-satellite tandem repeats wheat |
| url | https://www.mdpi.com/2073-4395/9/2/60 |
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