Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study
The present study was cherished to investigate in vitro thrombolytic, membrane stabilizing and antibacterial activities of Allamanda neriifolia and Aegialitis rotundifolia. Different types methanolic extracts of these two medicinal plants were tested for determining membrane stabilizing activity at...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411017300391 |
| _version_ | 1819264782994243584 |
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| author | Imam Hasan Md. Saddam Hussain Md. Shalahuddin Millat Niloy Sen Md. Abdur Rahman Md. Atikur Rahman Safiqul Islam Md. Mizanur Rahman Moghal |
| author_facet | Imam Hasan Md. Saddam Hussain Md. Shalahuddin Millat Niloy Sen Md. Abdur Rahman Md. Atikur Rahman Safiqul Islam Md. Mizanur Rahman Moghal |
| author_sort | Imam Hasan |
| collection | DOAJ |
| description | The present study was cherished to investigate in vitro thrombolytic, membrane stabilizing and antibacterial activities of Allamanda neriifolia and Aegialitis rotundifolia. Different types methanolic extracts of these two medicinal plants were tested for determining membrane stabilizing activity at a hypotonic solution and heat induce condition by comparing with reference standard acetyl salicylic acid (0.10 mg/mL), where thrombolytic activity assessment was done by employing Streptokinase as standard drug. Finally, antibacterial activity was performed against Staphylococcus aureus as a Gram-positive (+ve) and Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa as Gram negative (−ve) bacteria by using disc diffusion method. In case of membrane stabilizing studies, crude methanolic extracts of A. neriifolia at 10 mg/ml concentration, more importantly, showed 45.80% & 23.52% whereas 10 mg/ml concentration of A. rotundifolia more significantly (p < 0.01) produced 38.40% and 27.04% inhibition of hemolysis for both experimental conditions. Dose-dependently increased activity was found in the thrombolytic study where 10 mg/ml concentration of both A. neriifolia and A. rotundifolia more significantly (p < 0.01) showed 41.91% and 32.76% lysis of clot respectively by in vitro clot lysis assay method. Crude methanolic extracts of A. rotundifolia did not show any suitable antibacterial property against the test bacteria. However, the gram positive (+ve) bacteria also seemed resistant against A. neriifolia extract but this crude methanolic extracts was found to generate moderate antibacterial action against gram-negative (−ve) bacteria. The obtained results confirmed the presence of thrombolytic, membrane stabilizing activity for both plant extract along with moderate antibacterial activity for A. neriifolia. |
| first_indexed | 2024-12-23T20:34:58Z |
| format | Article |
| id | doaj.art-29844a542e164d8f88f20eb1232ff2c4 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-23T20:34:58Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-29844a542e164d8f88f20eb1232ff2c42022-12-21T17:32:06ZengElsevierJournal of Traditional and Complementary Medicine2225-41102018-01-018110711210.1016/j.jtcme.2017.03.005Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro studyImam Hasan0Md. Saddam Hussain1Md. Shalahuddin Millat2Niloy Sen3Md. Abdur Rahman4Md. Atikur Rahman5Safiqul Islam6Md. Mizanur Rahman Moghal7Department of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, 3814, Noakhali, BangladeshDepartment of Pharmacy, Mawlana Bhashani Science and Technology University, Santosh, Tangail, 1902, BangladeshThe present study was cherished to investigate in vitro thrombolytic, membrane stabilizing and antibacterial activities of Allamanda neriifolia and Aegialitis rotundifolia. Different types methanolic extracts of these two medicinal plants were tested for determining membrane stabilizing activity at a hypotonic solution and heat induce condition by comparing with reference standard acetyl salicylic acid (0.10 mg/mL), where thrombolytic activity assessment was done by employing Streptokinase as standard drug. Finally, antibacterial activity was performed against Staphylococcus aureus as a Gram-positive (+ve) and Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa as Gram negative (−ve) bacteria by using disc diffusion method. In case of membrane stabilizing studies, crude methanolic extracts of A. neriifolia at 10 mg/ml concentration, more importantly, showed 45.80% & 23.52% whereas 10 mg/ml concentration of A. rotundifolia more significantly (p < 0.01) produced 38.40% and 27.04% inhibition of hemolysis for both experimental conditions. Dose-dependently increased activity was found in the thrombolytic study where 10 mg/ml concentration of both A. neriifolia and A. rotundifolia more significantly (p < 0.01) showed 41.91% and 32.76% lysis of clot respectively by in vitro clot lysis assay method. Crude methanolic extracts of A. rotundifolia did not show any suitable antibacterial property against the test bacteria. However, the gram positive (+ve) bacteria also seemed resistant against A. neriifolia extract but this crude methanolic extracts was found to generate moderate antibacterial action against gram-negative (−ve) bacteria. The obtained results confirmed the presence of thrombolytic, membrane stabilizing activity for both plant extract along with moderate antibacterial activity for A. neriifolia.http://www.sciencedirect.com/science/article/pii/S2225411017300391Allamanda neriifoliaAegialitis rotundifoliaThrombolyticMembrane stabilizingAntibacterial |
| spellingShingle | Imam Hasan Md. Saddam Hussain Md. Shalahuddin Millat Niloy Sen Md. Abdur Rahman Md. Atikur Rahman Safiqul Islam Md. Mizanur Rahman Moghal Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study Journal of Traditional and Complementary Medicine Allamanda neriifolia Aegialitis rotundifolia Thrombolytic Membrane stabilizing Antibacterial |
| title | Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study |
| title_full | Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study |
| title_fullStr | Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study |
| title_full_unstemmed | Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study |
| title_short | Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study |
| title_sort | ascertainment of pharmacological activities of allamanda neriifolia hook and aegialitis rotundifolia roxb used in bangladesh an in vitro study |
| topic | Allamanda neriifolia Aegialitis rotundifolia Thrombolytic Membrane stabilizing Antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S2225411017300391 |
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