Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice
We previously reported the potential anti-obesity effects of the water extract of <i>Hydrangea serrata</i> (Thunb.) Ser. leaves (WHS) in high-fat diet-induced obese mice. As an extension of our previous study, we investigated the anti-adipogenic and anti-obesity effects of WHS and its un...
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MDPI AG
2021-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/10/3624 |
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| author | Hee-Soo Han Kyung-Sook Chung Yu-Kyoung Shin Sun Hee Lee Kyung-Tae Lee |
| author_facet | Hee-Soo Han Kyung-Sook Chung Yu-Kyoung Shin Sun Hee Lee Kyung-Tae Lee |
| author_sort | Hee-Soo Han |
| collection | DOAJ |
| description | We previously reported the potential anti-obesity effects of the water extract of <i>Hydrangea serrata</i> (Thunb.) Ser. leaves (WHS) in high-fat diet-induced obese mice. As an extension of our previous study, we investigated the anti-adipogenic and anti-obesity effects of WHS and its underlying molecular mechanisms in 3T3-L1 preadipocytes and genetically obese <i>db/db</i> mice. WHS attenuated the gene expression of adipogenic transcription factors, CCAAT/enhancer binding protein (C/EBP)α, peroxisome proliferator-activated receptor (PPAR)γ, and sterol regulatory element binding protein (SREBP)-1. Moreover, WHS inhibited the mitotic clonal expansion of preadipocytes by inducing G<sub>1</sub> cell cycle arrest. Oral administration of WHS alleviated body weight gain and body fat accumulation in vivo. In addition, adipocyte hypertrophy and liver steatosis were ameliorated by WHS treatment. WHS reduced C/EBPα, PPARγ, and SREBP-1 expression and activated AMPKα phosphorylation in both white adipose tissue (WAT) and liver tissue. WHS also mildly upregulated the expression of thermogenic proteins, including uncoupling protein-1, PPARs, PPARγ coactivator-1α, and sirtuin-1, in brown adipose tissue (BAT). Furthermore, WHS altered the gut microbiota composition to resemble that of wild-type mice. Taken together, our findings suggest that WHS could alleviate adiposity by inhibiting adipogenesis in WAT and the liver and modulating the gut microbiota. |
| first_indexed | 2024-03-10T06:17:59Z |
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| language | English |
| last_indexed | 2024-03-10T06:17:59Z |
| publishDate | 2021-10-01 |
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| series | Nutrients |
| spelling | doaj.art-2985ae48fffc42cf9aab44103fabd0452023-11-22T19:31:38ZengMDPI AGNutrients2072-66432021-10-011310362410.3390/nu13103624Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> MiceHee-Soo Han0Kyung-Sook Chung1Yu-Kyoung Shin2Sun Hee Lee3Kyung-Tae Lee4Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, KoreaDepartment of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, KoreaDepartment of New Material Development, COSMAXBIO, Seongnam 13486, KoreaDepartment of New Material Development, COSMAXBIO, Seongnam 13486, KoreaDepartment of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 02447, KoreaWe previously reported the potential anti-obesity effects of the water extract of <i>Hydrangea serrata</i> (Thunb.) Ser. leaves (WHS) in high-fat diet-induced obese mice. As an extension of our previous study, we investigated the anti-adipogenic and anti-obesity effects of WHS and its underlying molecular mechanisms in 3T3-L1 preadipocytes and genetically obese <i>db/db</i> mice. WHS attenuated the gene expression of adipogenic transcription factors, CCAAT/enhancer binding protein (C/EBP)α, peroxisome proliferator-activated receptor (PPAR)γ, and sterol regulatory element binding protein (SREBP)-1. Moreover, WHS inhibited the mitotic clonal expansion of preadipocytes by inducing G<sub>1</sub> cell cycle arrest. Oral administration of WHS alleviated body weight gain and body fat accumulation in vivo. In addition, adipocyte hypertrophy and liver steatosis were ameliorated by WHS treatment. WHS reduced C/EBPα, PPARγ, and SREBP-1 expression and activated AMPKα phosphorylation in both white adipose tissue (WAT) and liver tissue. WHS also mildly upregulated the expression of thermogenic proteins, including uncoupling protein-1, PPARs, PPARγ coactivator-1α, and sirtuin-1, in brown adipose tissue (BAT). Furthermore, WHS altered the gut microbiota composition to resemble that of wild-type mice. Taken together, our findings suggest that WHS could alleviate adiposity by inhibiting adipogenesis in WAT and the liver and modulating the gut microbiota.https://www.mdpi.com/2072-6643/13/10/3624<i>Hydrangea serrata</i> (Thunb.) Ser.obesity<i>db/db</i>adipogenesisthermogenesisgut microbiota |
| spellingShingle | Hee-Soo Han Kyung-Sook Chung Yu-Kyoung Shin Sun Hee Lee Kyung-Tae Lee Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice Nutrients <i>Hydrangea serrata</i> (Thunb.) Ser. obesity <i>db/db</i> adipogenesis thermogenesis gut microbiota |
| title | Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice |
| title_full | Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice |
| title_fullStr | Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice |
| title_full_unstemmed | Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice |
| title_short | Standardized <i>Hydrangea serrata</i> (Thunb.) Ser. Extract Ameliorates Obesity in <i>db/db</i> Mice |
| title_sort | standardized i hydrangea serrata i thunb ser extract ameliorates obesity in i db db i mice |
| topic | <i>Hydrangea serrata</i> (Thunb.) Ser. obesity <i>db/db</i> adipogenesis thermogenesis gut microbiota |
| url | https://www.mdpi.com/2072-6643/13/10/3624 |
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