Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development
The liver, a crucial metabolic organ in animals, is responsible for the synthesis, breakdown, and transport of lipids. However, the regulatory mechanisms involving both coding and noncoding RNAs that oversee the development of the goose liver remain elusive. This study aimed to fill this knowledge g...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/6/839 |
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| author | Shuibing Liu Chuan Li Xiaolong Hu Huirong Mao Sanfeng Liu Biao Chen |
| author_facet | Shuibing Liu Chuan Li Xiaolong Hu Huirong Mao Sanfeng Liu Biao Chen |
| author_sort | Shuibing Liu |
| collection | DOAJ |
| description | The liver, a crucial metabolic organ in animals, is responsible for the synthesis, breakdown, and transport of lipids. However, the regulatory mechanisms involving both coding and noncoding RNAs that oversee the development of the goose liver remain elusive. This study aimed to fill this knowledge gap by conducting RNA-seq to profile the expression of circular RNAs (circRNAs) and microRNAs (miRNAs) during goose liver development. We analyzed circRNAs in liver samples from Sichuan white geese at three developmental stages: posthatching day 0, 10 weeks (fast growth stage), and 30 weeks (sexual maturation stage). Our findings revealed 11,079 circRNAs and 994 miRNAs, among which the differentially expressed circRNAs and miRNAs were significantly enriched in pathways such as fatty acid biosynthesis, degradation, and metabolism. Further analysis of the target genes of the differentially expressed miRNAs revealed enrichment in pathways related to fatty acid biosynthesis, metabolism, PPAR signaling, DNA replication, and the cell cycle. We also established circRNA–miRNA–mRNA regulatory networks, identifying key regulatory factors and miRNAs. In conclusion, our study offers valuable insights into the complex interplay of circRNA–miRNA–mRNA interactions during goose liver development, and illuminates the molecular pathways that regulate this vital life function. |
| first_indexed | 2024-04-24T18:37:26Z |
| format | Article |
| id | doaj.art-2987674237454919aa9a135601234356 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-24T18:37:26Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-2987674237454919aa9a1356012343562024-03-27T13:17:37ZengMDPI AGAnimals2076-26152024-03-0114683910.3390/ani14060839Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver DevelopmentShuibing Liu0Chuan Li1Xiaolong Hu2Huirong Mao3Sanfeng Liu4Biao Chen5College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaThe liver, a crucial metabolic organ in animals, is responsible for the synthesis, breakdown, and transport of lipids. However, the regulatory mechanisms involving both coding and noncoding RNAs that oversee the development of the goose liver remain elusive. This study aimed to fill this knowledge gap by conducting RNA-seq to profile the expression of circular RNAs (circRNAs) and microRNAs (miRNAs) during goose liver development. We analyzed circRNAs in liver samples from Sichuan white geese at three developmental stages: posthatching day 0, 10 weeks (fast growth stage), and 30 weeks (sexual maturation stage). Our findings revealed 11,079 circRNAs and 994 miRNAs, among which the differentially expressed circRNAs and miRNAs were significantly enriched in pathways such as fatty acid biosynthesis, degradation, and metabolism. Further analysis of the target genes of the differentially expressed miRNAs revealed enrichment in pathways related to fatty acid biosynthesis, metabolism, PPAR signaling, DNA replication, and the cell cycle. We also established circRNA–miRNA–mRNA regulatory networks, identifying key regulatory factors and miRNAs. In conclusion, our study offers valuable insights into the complex interplay of circRNA–miRNA–mRNA interactions during goose liver development, and illuminates the molecular pathways that regulate this vital life function.https://www.mdpi.com/2076-2615/14/6/839goose liverRNA-seqceRNA regulatory networkscircRNAmiRNA |
| spellingShingle | Shuibing Liu Chuan Li Xiaolong Hu Huirong Mao Sanfeng Liu Biao Chen Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development Animals goose liver RNA-seq ceRNA regulatory networks circRNA miRNA |
| title | Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development |
| title_full | Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development |
| title_fullStr | Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development |
| title_full_unstemmed | Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development |
| title_short | Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development |
| title_sort | molecular mechanisms of circrna mirna mrna interactions in the regulation of goose liver development |
| topic | goose liver RNA-seq ceRNA regulatory networks circRNA miRNA |
| url | https://www.mdpi.com/2076-2615/14/6/839 |
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