Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
The role of the accompanying microbiota in the presence of <i>Listeria monocytogenes</i> on meat processing surfaces is not yet understood, especially in industrial production conditions. In this study, 300 conveyor belt samples from the cutting room of a swine slaughterhouse were collec...
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MDPI AG
2021-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/11/1368 |
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| author | Fanie Shedleur-Bourguignon William P. Thériault Jessie Longpré Alexandre Thibodeau Philippe Fravalo |
| author_facet | Fanie Shedleur-Bourguignon William P. Thériault Jessie Longpré Alexandre Thibodeau Philippe Fravalo |
| author_sort | Fanie Shedleur-Bourguignon |
| collection | DOAJ |
| description | The role of the accompanying microbiota in the presence of <i>Listeria monocytogenes</i> on meat processing surfaces is not yet understood, especially in industrial production conditions. In this study, 300 conveyor belt samples from the cutting room of a swine slaughterhouse were collected during production. The samples were subjected to the detection of <i>L. monocytogenes</i>. Recovered strains were characterized by serogrouping-PCR, InlA Sanger sequencing and for their ability to form biofilm. A selection of isolates was compared with core genome multi-locus sequence typing analysis (cgMLST). The sequencing of the V4 region of the 16S RNA gene of the microorganisms harvested from each sample was carried out in parallel using the Illumina MiSeq platform. Diversity analyses were performed and MaAsLin analysis was used to assess the link between <i>L. monocytogenes</i> detection and the surrounding bacteria. The 72 isolates collected showed a low genetic diversity and important persistence characteristics. <i>L. monocytogenes</i> isolates were not stochastically distributed on the surfaces: the isolates were detected on three out of six production lines, each associated with a specific meat cut: the half carcasses, the bostons and the picnics. MaAsLin biomarker analysis identified the taxa <i>Veillonella</i> (<i>p</i> ≤ 0.0397) as a bacterial determinant of the presence of <i>L. monocytogenes</i> on processing surfaces. The results of this study revealed a heterogenous contamination pattern of the processing surfaces by <i>L. monocytogenes</i> and targeted a bacterial indicator of the presence of the pathogen. These results could lead to a better risk assessment of the contamination of meat products. |
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| language | English |
| last_indexed | 2024-03-10T05:10:39Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-2987ebd4aa1244a68807c9c9d5ccd4222023-11-23T00:52:15ZengMDPI AGPathogens2076-08172021-10-011011136810.3390/pathogens10111368Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, CanadaFanie Shedleur-Bourguignon0William P. Thériault1Jessie Longpré2Alexandre Thibodeau3Philippe Fravalo4NSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaF. Ménard, Division d’Olymel s.e.c., Ange-Gardien, QC J0E 1E0, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaThe role of the accompanying microbiota in the presence of <i>Listeria monocytogenes</i> on meat processing surfaces is not yet understood, especially in industrial production conditions. In this study, 300 conveyor belt samples from the cutting room of a swine slaughterhouse were collected during production. The samples were subjected to the detection of <i>L. monocytogenes</i>. Recovered strains were characterized by serogrouping-PCR, InlA Sanger sequencing and for their ability to form biofilm. A selection of isolates was compared with core genome multi-locus sequence typing analysis (cgMLST). The sequencing of the V4 region of the 16S RNA gene of the microorganisms harvested from each sample was carried out in parallel using the Illumina MiSeq platform. Diversity analyses were performed and MaAsLin analysis was used to assess the link between <i>L. monocytogenes</i> detection and the surrounding bacteria. The 72 isolates collected showed a low genetic diversity and important persistence characteristics. <i>L. monocytogenes</i> isolates were not stochastically distributed on the surfaces: the isolates were detected on three out of six production lines, each associated with a specific meat cut: the half carcasses, the bostons and the picnics. MaAsLin biomarker analysis identified the taxa <i>Veillonella</i> (<i>p</i> ≤ 0.0397) as a bacterial determinant of the presence of <i>L. monocytogenes</i> on processing surfaces. The results of this study revealed a heterogenous contamination pattern of the processing surfaces by <i>L. monocytogenes</i> and targeted a bacterial indicator of the presence of the pathogen. These results could lead to a better risk assessment of the contamination of meat products.https://www.mdpi.com/2076-0817/10/11/1368<i>Listeria monocytogenes</i>accompanying microbiotaconveyor belt surfacesheterogeneous spatial contamination<i>Veillonella</i> |
| spellingShingle | Fanie Shedleur-Bourguignon William P. Thériault Jessie Longpré Alexandre Thibodeau Philippe Fravalo Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada Pathogens <i>Listeria monocytogenes</i> accompanying microbiota conveyor belt surfaces heterogeneous spatial contamination <i>Veillonella</i> |
| title | Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada |
| title_full | Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada |
| title_fullStr | Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada |
| title_full_unstemmed | Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada |
| title_short | Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada |
| title_sort | use of an ecosystem based approach to shed light on the heterogeneity of the contamination pattern of i listeria monocytogenes i on conveyor belt surfaces in a swine slaughterhouse in the province of quebec canada |
| topic | <i>Listeria monocytogenes</i> accompanying microbiota conveyor belt surfaces heterogeneous spatial contamination <i>Veillonella</i> |
| url | https://www.mdpi.com/2076-0817/10/11/1368 |
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