Detection of membrane-anchoring lipid modifications of proteins in cells by radioactive metabolic labeling

Detection of membrane-anchoring lipid modifications of proteins in cells by radioactive metabolic labeling

Summary: Prenylation and palmitoylation are two major lipid modifications of cellular proteins that anchor proteins to cell membranes. Here, we present a protocol for detecting these modifications in cellular proteins by radioactive metabolic labeling. We describe steps for metabolic labeling of cel...

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Bibliographic Details
Main Authors:	Sameer Ahmed Bhat, Rachel Ferguson, Ritika Adhikari, Shafi Kuchay
Format:	Article
Language:	English
Published:	Elsevier 2023-09-01
Series:	STAR Protocols
Subjects:	Cell Membrane Cell-based Assays Protein Expression and Purification
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723003830

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