Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum)
Changes in the activity of acid phosphatase (AcPase) in the apoplast of pea root nodule were investigated. The activity was determined using lead and cerium methods. The results indicated a following sequence of AcPase activity appearance during the development of the infection thread: 1) low AcPase...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2011-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/516 |
| _version_ | 1828811503251226624 |
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| author | Marzena Sujkowska Wojciech Borucki Władysław Golinowski |
| author_facet | Marzena Sujkowska Wojciech Borucki Władysław Golinowski |
| author_sort | Marzena Sujkowska |
| collection | DOAJ |
| description | Changes in the activity of acid phosphatase (AcPase) in the apoplast of pea root nodule were investigated. The activity was determined using lead and cerium methods. The results indicated a following sequence of AcPase activity appearance during the development of the infection thread: 1) low AcPase activity appears in the outer part of cells of symbiotic bacteria; 2) bacteria show increased AcPase activity, and the enzyme activity appears in the thread walls; 3) activity exhibits also matrix of the infection thread; 4) bacteria just before their release from the infection threads show high AcPase activity; 5) AcPase activity ceases after bacteria transformation into bacteroids. The increase in bacterial AcPase activity may reflect a higher demand for inorganic phosphorus necessary for propagation of the bacteria within the infection threads and/or involved in bacteria release from the infection threads. |
| first_indexed | 2024-12-12T09:28:52Z |
| format | Article |
| id | doaj.art-2989cdd170d040df80ffc65dc78f80ed |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T09:28:52Z |
| publishDate | 2011-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-2989cdd170d040df80ffc65dc78f80ed2022-12-22T00:28:56ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-01751333810.5586/asbp.2006.006321Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum)Marzena Sujkowska0Wojciech Borucki1Władysław Golinowski2Agricultural University of WarsawAgricultural University of WarsawAgricultural University of WarsawChanges in the activity of acid phosphatase (AcPase) in the apoplast of pea root nodule were investigated. The activity was determined using lead and cerium methods. The results indicated a following sequence of AcPase activity appearance during the development of the infection thread: 1) low AcPase activity appears in the outer part of cells of symbiotic bacteria; 2) bacteria show increased AcPase activity, and the enzyme activity appears in the thread walls; 3) activity exhibits also matrix of the infection thread; 4) bacteria just before their release from the infection threads show high AcPase activity; 5) AcPase activity ceases after bacteria transformation into bacteroids. The increase in bacterial AcPase activity may reflect a higher demand for inorganic phosphorus necessary for propagation of the bacteria within the infection threads and/or involved in bacteria release from the infection threads.https://pbsociety.org.pl/journals/index.php/asbp/article/view/516acid phosphataselocalizationinfection threadnodulePisum sativum |
| spellingShingle | Marzena Sujkowska Wojciech Borucki Władysław Golinowski Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum) Acta Societatis Botanicorum Poloniae acid phosphatase localization infection thread nodule Pisum sativum |
| title | Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum) |
| title_full | Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum) |
| title_fullStr | Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum) |
| title_full_unstemmed | Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum) |
| title_short | Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum) |
| title_sort | localization of acid phosphatase activity in the apoplast of root nodules of pea pisum sativum |
| topic | acid phosphatase localization infection thread nodule Pisum sativum |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/516 |
| work_keys_str_mv | AT marzenasujkowska localizationofacidphosphataseactivityintheapoplastofrootnodulesofpeapisumsativum AT wojciechborucki localizationofacidphosphataseactivityintheapoplastofrootnodulesofpeapisumsativum AT władysławgolinowski localizationofacidphosphataseactivityintheapoplastofrootnodulesofpeapisumsativum |