Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide
Cyclophosphamide (CP)—which is used to treat autoimmune diseases and cancer—is related to gonadotoxicity attributed to oxidative stress. As phycobiliproteins (PBPs) are strong antioxidants that are unexplored as protective agents against male gonadotoxicity, our work aimed to investigate the effects...
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MDPI AG
2021-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/8/2616 |
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| author | Jorge Briseño-Bugarín Isabel Hernández-Ochoa Xelha Araujo-Padilla María Angélica Mojica-Villegas Ricardo Iván Montaño-González Gabriela Gutiérrez-Salmeán Germán Chamorro-Cevallos |
| author_facet | Jorge Briseño-Bugarín Isabel Hernández-Ochoa Xelha Araujo-Padilla María Angélica Mojica-Villegas Ricardo Iván Montaño-González Gabriela Gutiérrez-Salmeán Germán Chamorro-Cevallos |
| author_sort | Jorge Briseño-Bugarín |
| collection | DOAJ |
| description | Cyclophosphamide (CP)—which is used to treat autoimmune diseases and cancer—is related to gonadotoxicity attributed to oxidative stress. As phycobiliproteins (PBPs) are strong antioxidants that are unexplored as protective agents against male gonadotoxicity, our work aimed to investigate the effects of PBP crude extract on testicular damage and sperm parameter alterations caused by CP in mice. Three doses of PBP (50, 100, and 200 mg/kg) were tested in the experimental groups (<i>n</i> = 8 per group), administered concomitantly with 100 mg/kg CP. After 42 days receiving PBP daily and CP weekly, body and relative testicular weights, serum testosterone levels, testicular lipoperoxidation and antioxidant enzyme activity levels, and testicular histology and sperm parameter alterations were assessed. The results showed that PBP crude extract at 200 mg/kg prevented testosterone serum reduction, body weight loss, lipoperoxidation and enzyme activity increments, and sperm parameter alterations and partially ameliorated relative testicular weight reductions and histological damage in CP-treated mice. In conclusion, we showed that PBP crude extract (200 mg/kg) mitigated oxidative damage in the testes and ameliorated alterations in sperm parameters in mice treated with CP (100 mg/kg); therefore, PBP extract could be considered as a potential protective agent against CP toxicity. |
| first_indexed | 2024-03-10T08:30:58Z |
| format | Article |
| id | doaj.art-298d18290b5c4fadbe184273a7360d90 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T08:30:58Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-298d18290b5c4fadbe184273a7360d902023-11-22T09:03:26ZengMDPI AGNutrients2072-66432021-07-01138261610.3390/nu13082616Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with CyclophosphamideJorge Briseño-Bugarín0Isabel Hernández-Ochoa1Xelha Araujo-Padilla2María Angélica Mojica-Villegas3Ricardo Iván Montaño-González4Gabriela Gutiérrez-Salmeán5Germán Chamorro-Cevallos6Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Zacatenco, Mexico City 07738, MexicoDepartamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN), Unidad Zacatenco, Mexico City 07360, MexicoDepartamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Zacatenco, Mexico City 07738, MexicoDepartamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Zacatenco, Mexico City 07738, MexicoDepartamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Zacatenco, Mexico City 07738, MexicoCentro de Investigación en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud, Universidad Anáhuac México, Mexico City 52786, MexicoDepartamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Zacatenco, Mexico City 07738, MexicoCyclophosphamide (CP)—which is used to treat autoimmune diseases and cancer—is related to gonadotoxicity attributed to oxidative stress. As phycobiliproteins (PBPs) are strong antioxidants that are unexplored as protective agents against male gonadotoxicity, our work aimed to investigate the effects of PBP crude extract on testicular damage and sperm parameter alterations caused by CP in mice. Three doses of PBP (50, 100, and 200 mg/kg) were tested in the experimental groups (<i>n</i> = 8 per group), administered concomitantly with 100 mg/kg CP. After 42 days receiving PBP daily and CP weekly, body and relative testicular weights, serum testosterone levels, testicular lipoperoxidation and antioxidant enzyme activity levels, and testicular histology and sperm parameter alterations were assessed. The results showed that PBP crude extract at 200 mg/kg prevented testosterone serum reduction, body weight loss, lipoperoxidation and enzyme activity increments, and sperm parameter alterations and partially ameliorated relative testicular weight reductions and histological damage in CP-treated mice. In conclusion, we showed that PBP crude extract (200 mg/kg) mitigated oxidative damage in the testes and ameliorated alterations in sperm parameters in mice treated with CP (100 mg/kg); therefore, PBP extract could be considered as a potential protective agent against CP toxicity.https://www.mdpi.com/2072-6643/13/8/2616phycobiliproteinscyclophosphamidetesticular damagesperm parameters |
| spellingShingle | Jorge Briseño-Bugarín Isabel Hernández-Ochoa Xelha Araujo-Padilla María Angélica Mojica-Villegas Ricardo Iván Montaño-González Gabriela Gutiérrez-Salmeán Germán Chamorro-Cevallos Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide Nutrients phycobiliproteins cyclophosphamide testicular damage sperm parameters |
| title | Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide |
| title_full | Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide |
| title_fullStr | Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide |
| title_full_unstemmed | Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide |
| title_short | Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide |
| title_sort | phycobiliproteins ameliorate gonadal toxicity in male mice treated with cyclophosphamide |
| topic | phycobiliproteins cyclophosphamide testicular damage sperm parameters |
| url | https://www.mdpi.com/2072-6643/13/8/2616 |
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