A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus
Background: Staphylococcus aureus (S. aureus) is an important human pathogen associated with hospital- and community-acquired diseases, a wide range of infectious disease including minor skin disease to more sever and aggressive infections such as septicemia, pneumonia, endocarditis, and deep skin a...
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| Format: | Article |
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Isfahan University of Medical Sciences
2013-06-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2608 |
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| author | Seyed Asghar Havaei Sharareh Moghim Mojtaba Shahin Amir Azimian Fahimeh Ghanbari Dariush Shokri Mojtaba Akbari Nafiseh Sadat Hoseini |
| author_facet | Seyed Asghar Havaei Sharareh Moghim Mojtaba Shahin Amir Azimian Fahimeh Ghanbari Dariush Shokri Mojtaba Akbari Nafiseh Sadat Hoseini |
| author_sort | Seyed Asghar Havaei |
| collection | DOAJ |
| description | Background: Staphylococcus aureus (S. aureus) is an important human pathogen associated with hospital- and community-acquired diseases, a wide range of infectious disease including minor skin disease to more sever and aggressive infections such as septicemia, pneumonia, endocarditis, and deep skin abscess. S. aureus has a high mortality rate of 35%. Therefore, it is crucial to identify the methicillin resistant S. aureus (MRSA) strains and implement the necessary treatment in order to control the spread of hospital infections.
Methods: A total of 150 S. aureus isolates were collected from samples of patients admitted to Alzahra and Shariati hospitals in Isfahan, Iran. Polymerase chain reaction (PCR) for mecA gene was performed in all strains. Thereafter, the minimum inhibitory concentration (MIC) for oxacillin was carried out using agar dilution method. Then, antibiogram using disk diffusion for cephoxitin was performed according to Clinical and Laboratory Standards Institute (CLSI) regulations on isolates containing mecA gene. The three methods were then compared.
Findings: Of the 150 samples, 62 samples (41.33%) were found to carry mecA gene using PCR. However, agar dilution for oxacillin found 56 of 62 samples (90.33%) to harbor mecA gene. Disk diffusion method revealed that 53 of 62 samples (85.48%) contain mecA gene.
Conclusion: Agar dilution method was found to have a higher sensitivity in determining antibiotic sensitivity compared to disk diffusion method. However, PCR was identified as the ideal method for detecting MRSA strains; since a number of strains were found to be sensitive to oxacillin in phenotypic test due to lack of mecA expression while still containing the gene. |
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| format | Article |
| id | doaj.art-298e999191a64659b28b8948eb4761e6 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:21:56Z |
| publishDate | 2013-06-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-298e999191a64659b28b8948eb4761e62023-09-02T14:27:23ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-06-01312324664741317A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus AureusSeyed Asghar Havaei0Sharareh Moghim1Mojtaba Shahin2Amir Azimian3Fahimeh Ghanbari4Dariush Shokri5Mojtaba Akbari6Nafiseh Sadat Hoseini7Associate Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Nosocomial Infection Research Center AND Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, IranMSc Student, Department of Microbiology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Patobiology and Anatomy, School of Medicine, North Khorasan University of Medical Sciences, Bojnourd, IranDepartment of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, IranPhD Student, Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, IranEpidemiologist, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranMSc Student, Department of Microbiology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranBackground: Staphylococcus aureus (S. aureus) is an important human pathogen associated with hospital- and community-acquired diseases, a wide range of infectious disease including minor skin disease to more sever and aggressive infections such as septicemia, pneumonia, endocarditis, and deep skin abscess. S. aureus has a high mortality rate of 35%. Therefore, it is crucial to identify the methicillin resistant S. aureus (MRSA) strains and implement the necessary treatment in order to control the spread of hospital infections. Methods: A total of 150 S. aureus isolates were collected from samples of patients admitted to Alzahra and Shariati hospitals in Isfahan, Iran. Polymerase chain reaction (PCR) for mecA gene was performed in all strains. Thereafter, the minimum inhibitory concentration (MIC) for oxacillin was carried out using agar dilution method. Then, antibiogram using disk diffusion for cephoxitin was performed according to Clinical and Laboratory Standards Institute (CLSI) regulations on isolates containing mecA gene. The three methods were then compared. Findings: Of the 150 samples, 62 samples (41.33%) were found to carry mecA gene using PCR. However, agar dilution for oxacillin found 56 of 62 samples (90.33%) to harbor mecA gene. Disk diffusion method revealed that 53 of 62 samples (85.48%) contain mecA gene. Conclusion: Agar dilution method was found to have a higher sensitivity in determining antibiotic sensitivity compared to disk diffusion method. However, PCR was identified as the ideal method for detecting MRSA strains; since a number of strains were found to be sensitive to oxacillin in phenotypic test due to lack of mecA expression while still containing the gene.http://jims.mui.ac.ir/index.php/jims/article/view/2608Staphylococcus aureusmecA geneMethicillinResistant |
| spellingShingle | Seyed Asghar Havaei Sharareh Moghim Mojtaba Shahin Amir Azimian Fahimeh Ghanbari Dariush Shokri Mojtaba Akbari Nafiseh Sadat Hoseini A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus مجله دانشکده پزشکی اصفهان Staphylococcus aureus mecA gene Methicillin Resistant |
| title | A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus |
| title_full | A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus |
| title_fullStr | A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus |
| title_full_unstemmed | A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus |
| title_short | A Comparison between Polymerase Chain Reaction, Oxacillin Agar Dilusion and Cefoxitin Disk Diffusion Methods in Detection of Methicillin Resistance in Staphylococcus Aureus |
| title_sort | comparison between polymerase chain reaction oxacillin agar dilusion and cefoxitin disk diffusion methods in detection of methicillin resistance in staphylococcus aureus |
| topic | Staphylococcus aureus mecA gene Methicillin Resistant |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2608 |
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