Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality
The purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. The material used for research were semen from two selected Simmental bulls. Vitamin E as treatment...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/02/e3sconf_icals2019_02002.pdf |
| _version_ | 1818644910741389312 |
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| author | Zharfan Haris Fariz Soepri Ondho Yon Samsudewa Daud |
| author_facet | Zharfan Haris Fariz Soepri Ondho Yon Samsudewa Daud |
| author_sort | Zharfan Haris Fariz |
| collection | DOAJ |
| description | The purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. The material used for research were semen from two selected Simmental bulls. Vitamin E as treatment in addition to the extender consisted of T0 (no addition of vitamin E), T1 (0,134 gram/100 ml extender), T2 (0,268 gram/100 ml extender) and T3 (0,402 gram/100 ml extender). Post-thawing evaluation conducted 24 hours after the freezing process and observed after the thawing process in 37°C water bath for 30 seconds. Parameters observed in this research were the post-thawing quality of frozen-thawed Simmental bull semen based on motility, mortality and abnormality percentage. Sperm motility evaluated using a microscope with 100x and 400x magnifying, sperm mortality observed using 400x magnifying and counted based on 0,2% eosin-negrosin staining, sperm abnormality observed using 400x magnifying and counted based on the number of morphologically abnormal sperm cells. Semen post-thawing motility was not significantly affected by vitamin E addition of T1, T2 and T3 (P<0,05). T1 and T2 were able to significantly lower the mortality percentage compared to T0 and T3 (P<0,05). T1 and T2 were also very significantly affecting the decreased of abnormality percentage compared to T0 and T3 (P<0,01). T1 generally gave the best result on the improvement of post-thawing motility and decreased the percentage of sperm mortality and abnormality. |
| first_indexed | 2024-12-17T00:22:22Z |
| format | Article |
| id | doaj.art-298f396b0f39420790487f6e2005c70d |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-17T00:22:22Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-298f396b0f39420790487f6e2005c70d2022-12-21T22:10:32ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011420200210.1051/e3sconf/202014202002e3sconf_icals2019_02002Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing qualityZharfan Haris FarizSoepri Ondho YonSamsudewa DaudThe purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. The material used for research were semen from two selected Simmental bulls. Vitamin E as treatment in addition to the extender consisted of T0 (no addition of vitamin E), T1 (0,134 gram/100 ml extender), T2 (0,268 gram/100 ml extender) and T3 (0,402 gram/100 ml extender). Post-thawing evaluation conducted 24 hours after the freezing process and observed after the thawing process in 37°C water bath for 30 seconds. Parameters observed in this research were the post-thawing quality of frozen-thawed Simmental bull semen based on motility, mortality and abnormality percentage. Sperm motility evaluated using a microscope with 100x and 400x magnifying, sperm mortality observed using 400x magnifying and counted based on 0,2% eosin-negrosin staining, sperm abnormality observed using 400x magnifying and counted based on the number of morphologically abnormal sperm cells. Semen post-thawing motility was not significantly affected by vitamin E addition of T1, T2 and T3 (P<0,05). T1 and T2 were able to significantly lower the mortality percentage compared to T0 and T3 (P<0,05). T1 and T2 were also very significantly affecting the decreased of abnormality percentage compared to T0 and T3 (P<0,01). T1 generally gave the best result on the improvement of post-thawing motility and decreased the percentage of sperm mortality and abnormality.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/02/e3sconf_icals2019_02002.pdf |
| spellingShingle | Zharfan Haris Fariz Soepri Ondho Yon Samsudewa Daud Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality E3S Web of Conferences |
| title | Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality |
| title_full | Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality |
| title_fullStr | Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality |
| title_full_unstemmed | Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality |
| title_short | Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality |
| title_sort | effect of vitamin e addition to frozen simmental bull semen extender on post thawing quality |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/02/e3sconf_icals2019_02002.pdf |
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