A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton
<p>Abstract</p> <p>In previous studies it was determined that the stable isotope 13-carbon can be used as a semen label to detect mating events in the malaria mosquito <it>Anopheles arabiensis</it>. In this paper we describe the use of an additional stable isotope, 15-n...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-07-01
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| Series: | Parasites & Vectors |
| Online Access: | http://www.parasitesandvectors.com/content/1/1/19 |
| _version_ | 1828288919018405888 |
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| author | Gludovacz Doris Hood Rebecca C Helinski Michelle EH Mayr Leo Knols Bart GJ |
| author_facet | Gludovacz Doris Hood Rebecca C Helinski Michelle EH Mayr Leo Knols Bart GJ |
| author_sort | Gludovacz Doris |
| collection | DOAJ |
| description | <p>Abstract</p> <p>In previous studies it was determined that the stable isotope 13-carbon can be used as a semen label to detect mating events in the malaria mosquito <it>Anopheles arabiensis</it>. In this paper we describe the use of an additional stable isotope, 15-nitrogen (<sup>15</sup>N), for that same purpose. Both stable isotopes can be analysed simultaneously in a mass spectrometer, offering the possibility to detect both labels in one sample in order to study complex and difficult-to-detect mating events, such as multiple mating. <sup>15</sup>N-glycine was added to larval rearing water and the target enrichment was 5 atom% <sup>15</sup>N. Males from these trays were mated with unlabelled virgin females, and spiked spermathecae were analysed for isotopic composition after mating using mass spectrometry. Results showed that spermathecae positive for semen could be distinguished from uninseminated or control samples using the raw δ<sup>15</sup>N‰ values. The label persisted in spermathecae for up to 5 days after insemination, and males aged 10 days transferred similar amounts of label as males aged 4 days. There were no negative effects of the label on larval survival and male longevity. Enrichment of teneral mosquitoes after emergence was 4.85 ± 0.10 atom% <sup>15</sup>N. A threshold value defined as 3 standard deviations above the mean of virgin (<it>i.e. </it>uninseminated spermathecae) samples was successful in classifying a large proportion of samples correctly (<it>i.e. </it>on average 95%). We conclude that alongside <sup>13</sup>C, <sup>15</sup>N can be used to detect mating in <it>Anopheles </it>and the suitability of both labels is briefly discussed.</p> |
| first_indexed | 2024-04-13T10:12:47Z |
| format | Article |
| id | doaj.art-298f3efedc1445b0960c63d2ab731985 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-13T10:12:47Z |
| publishDate | 2008-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-298f3efedc1445b0960c63d2ab7319852022-12-22T02:50:52ZengBMCParasites & Vectors1756-33052008-07-01111910.1186/1756-3305-1-19A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>PattonGludovacz DorisHood Rebecca CHelinski Michelle EHMayr LeoKnols Bart GJ<p>Abstract</p> <p>In previous studies it was determined that the stable isotope 13-carbon can be used as a semen label to detect mating events in the malaria mosquito <it>Anopheles arabiensis</it>. In this paper we describe the use of an additional stable isotope, 15-nitrogen (<sup>15</sup>N), for that same purpose. Both stable isotopes can be analysed simultaneously in a mass spectrometer, offering the possibility to detect both labels in one sample in order to study complex and difficult-to-detect mating events, such as multiple mating. <sup>15</sup>N-glycine was added to larval rearing water and the target enrichment was 5 atom% <sup>15</sup>N. Males from these trays were mated with unlabelled virgin females, and spiked spermathecae were analysed for isotopic composition after mating using mass spectrometry. Results showed that spermathecae positive for semen could be distinguished from uninseminated or control samples using the raw δ<sup>15</sup>N‰ values. The label persisted in spermathecae for up to 5 days after insemination, and males aged 10 days transferred similar amounts of label as males aged 4 days. There were no negative effects of the label on larval survival and male longevity. Enrichment of teneral mosquitoes after emergence was 4.85 ± 0.10 atom% <sup>15</sup>N. A threshold value defined as 3 standard deviations above the mean of virgin (<it>i.e. </it>uninseminated spermathecae) samples was successful in classifying a large proportion of samples correctly (<it>i.e. </it>on average 95%). We conclude that alongside <sup>13</sup>C, <sup>15</sup>N can be used to detect mating in <it>Anopheles </it>and the suitability of both labels is briefly discussed.</p>http://www.parasitesandvectors.com/content/1/1/19 |
| spellingShingle | Gludovacz Doris Hood Rebecca C Helinski Michelle EH Mayr Leo Knols Bart GJ A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton Parasites & Vectors |
| title | A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton |
| title_full | A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton |
| title_fullStr | A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton |
| title_full_unstemmed | A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton |
| title_short | A <sup>15</sup>N stable isotope semen label to detect mating in the malaria mosquito <it>Anopheles arabiensis </it>Patton |
| title_sort | sup 15 sup n stable isotope semen label to detect mating in the malaria mosquito it anopheles arabiensis it patton |
| url | http://www.parasitesandvectors.com/content/1/1/19 |
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