Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor
It is expected that human iPS cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat serious heart diseases. However, the properties and functions of human adult cardiomyocytes and hiPSC-CMs, including cell maturation, differ. In this study, we focused on the temperature dependence of hiPSC-CM...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/7/3370 |
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| author | Ryota Ikegami Takuya Tsukagoshi Kenei Matsudaira Kayoko Hirayama Shoji Hidetoshi Takahashi Thanh-Vinh Nguyen Takumi Tamamoto Kentaro Noda Ken’ichi Koyanagi Toru Oshima Isao Shimoyama |
| author_facet | Ryota Ikegami Takuya Tsukagoshi Kenei Matsudaira Kayoko Hirayama Shoji Hidetoshi Takahashi Thanh-Vinh Nguyen Takumi Tamamoto Kentaro Noda Ken’ichi Koyanagi Toru Oshima Isao Shimoyama |
| author_sort | Ryota Ikegami |
| collection | DOAJ |
| description | It is expected that human iPS cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat serious heart diseases. However, the properties and functions of human adult cardiomyocytes and hiPSC-CMs, including cell maturation, differ. In this study, we focused on the temperature dependence of hiPSC-CMs by integrating the temperature regulation system into our sensor platform, which can directly and quantitatively measure their mechanical motion. We measured the beating frequency of hiPSC-CMs at different environmental temperatures and found that the beating frequency increased as the temperature increased. Although the rate at which the beating frequency increased with temperature varied, the temperature at which the beating stopped was relatively stable at approximately 20 °C. The stopping of beating at this temperature was stable, even in immature hiPSC-CMs, and was considered to be a primitive property of cardiomyocytes. |
| first_indexed | 2024-03-11T05:25:34Z |
| format | Article |
| id | doaj.art-2990c9a1e9094522b46687a4d8585f1c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T05:25:34Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-2990c9a1e9094522b46687a4d8585f1c2023-11-17T17:31:31ZengMDPI AGSensors1424-82202023-03-01237337010.3390/s23073370Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force SensorRyota Ikegami0Takuya Tsukagoshi1Kenei Matsudaira2Kayoko Hirayama Shoji3Hidetoshi Takahashi4Thanh-Vinh Nguyen5Takumi Tamamoto6Kentaro Noda7Ken’ichi Koyanagi8Toru Oshima9Isao Shimoyama10Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, JapanDepartment of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, JapanDepartment of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, JapanDepartment of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, JapanDepartment of Mechanical Engineering, Faculty of Science and Technology, Keio University, Yokohama 223-8522, JapanSensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8564, JapanDepartment of Intelligent Mechanical Engineering, Faculty of Engineering, Fukuoka Institute of Technology, Fukuoka 811-0295, JapanDepartment of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, JapanDepartment of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, JapanDepartment of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, JapanToyama Prefectural University, Imizu 939-0398, JapanIt is expected that human iPS cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat serious heart diseases. However, the properties and functions of human adult cardiomyocytes and hiPSC-CMs, including cell maturation, differ. In this study, we focused on the temperature dependence of hiPSC-CMs by integrating the temperature regulation system into our sensor platform, which can directly and quantitatively measure their mechanical motion. We measured the beating frequency of hiPSC-CMs at different environmental temperatures and found that the beating frequency increased as the temperature increased. Although the rate at which the beating frequency increased with temperature varied, the temperature at which the beating stopped was relatively stable at approximately 20 °C. The stopping of beating at this temperature was stable, even in immature hiPSC-CMs, and was considered to be a primitive property of cardiomyocytes.https://www.mdpi.com/1424-8220/23/7/3370piezoresistive cantilevercardiomyocytestemperature dependence |
| spellingShingle | Ryota Ikegami Takuya Tsukagoshi Kenei Matsudaira Kayoko Hirayama Shoji Hidetoshi Takahashi Thanh-Vinh Nguyen Takumi Tamamoto Kentaro Noda Ken’ichi Koyanagi Toru Oshima Isao Shimoyama Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor Sensors piezoresistive cantilever cardiomyocytes temperature dependence |
| title | Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor |
| title_full | Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor |
| title_fullStr | Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor |
| title_full_unstemmed | Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor |
| title_short | Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor |
| title_sort | temperature dependence of the beating frequency of hipsc cms using a mems force sensor |
| topic | piezoresistive cantilever cardiomyocytes temperature dependence |
| url | https://www.mdpi.com/1424-8220/23/7/3370 |
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