Protocol Standardization Reveals MV Correlation to Healthy Donor BMI
Microvesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early signs of disease and/or of treatment progression. However, there are growing concerns about using conventional flow cytometry (cFMC) for the detection and quantification of m...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AboutScience Srl
2014-01-01
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| Series: | Journal of Circulating Biomarkers |
| Online Access: | https://doi.org/10.5772/58527 |
| _version_ | 1811191803156627456 |
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| author | Philip Hexley Kyle P. Rismiller Chad T. Robinson George F. Babcock |
| author_facet | Philip Hexley Kyle P. Rismiller Chad T. Robinson George F. Babcock |
| author_sort | Philip Hexley |
| collection | DOAJ |
| description | Microvesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early signs of disease and/or of treatment progression. However, there are growing concerns about using conventional flow cytometry (cFMC) for the detection and quantification of microvesicles. These concerns range from error-sources in collection through to the physical limitations of detection. Here we present a standardized method for collection and analysis which shows that the MV numbers detected by cFCM correlate to donor Body Mass Index (BMI). Although unlikely to be comprehensive, we also demonstrate how cFCM is a useful and valid tool in the analysis of MVs. |
| first_indexed | 2024-04-11T23:41:51Z |
| format | Article |
| id | doaj.art-2991945c8e6942c8879983bab502d6ba |
| institution | Directory Open Access Journal |
| issn | 1849-4544 |
| language | English |
| last_indexed | 2024-04-11T23:41:51Z |
| publishDate | 2014-01-01 |
| publisher | AboutScience Srl |
| record_format | Article |
| series | Journal of Circulating Biomarkers |
| spelling | doaj.art-2991945c8e6942c8879983bab502d6ba2022-12-22T03:56:46ZengAboutScience SrlJournal of Circulating Biomarkers1849-45442014-01-01210.5772/58527Protocol Standardization Reveals MV Correlation to Healthy Donor BMIPhilip Hexley0Kyle P. Rismiller1Chad T. Robinson2George F. Babcock3 University of Nebraska Medical Center, Omaha, NE University of Dayton. Dayton, OH University of Cincinnati. Cincinnati, OH Shriners Hospitals for Children, Cincinnati, OHMicrovesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early signs of disease and/or of treatment progression. However, there are growing concerns about using conventional flow cytometry (cFMC) for the detection and quantification of microvesicles. These concerns range from error-sources in collection through to the physical limitations of detection. Here we present a standardized method for collection and analysis which shows that the MV numbers detected by cFCM correlate to donor Body Mass Index (BMI). Although unlikely to be comprehensive, we also demonstrate how cFCM is a useful and valid tool in the analysis of MVs.https://doi.org/10.5772/58527 |
| spellingShingle | Philip Hexley Kyle P. Rismiller Chad T. Robinson George F. Babcock Protocol Standardization Reveals MV Correlation to Healthy Donor BMI Journal of Circulating Biomarkers |
| title | Protocol Standardization Reveals MV Correlation to Healthy Donor BMI |
| title_full | Protocol Standardization Reveals MV Correlation to Healthy Donor BMI |
| title_fullStr | Protocol Standardization Reveals MV Correlation to Healthy Donor BMI |
| title_full_unstemmed | Protocol Standardization Reveals MV Correlation to Healthy Donor BMI |
| title_short | Protocol Standardization Reveals MV Correlation to Healthy Donor BMI |
| title_sort | protocol standardization reveals mv correlation to healthy donor bmi |
| url | https://doi.org/10.5772/58527 |
| work_keys_str_mv | AT philiphexley protocolstandardizationrevealsmvcorrelationtohealthydonorbmi AT kyleprismiller protocolstandardizationrevealsmvcorrelationtohealthydonorbmi AT chadtrobinson protocolstandardizationrevealsmvcorrelationtohealthydonorbmi AT georgefbabcock protocolstandardizationrevealsmvcorrelationtohealthydonorbmi |