Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro
The optimum conditions for Ceruloplasmin (CP) activity have been measured in serum samples of a healthy individual without any detectable diseases. CP activity was evaluated by measurement of its p-phenylenediamine oxidase activity. This study showed the optimum pHforCP activity of a healthy human s...
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| Format: | Article |
| Language: | English |
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Unviversity of Technology- Iraq
2016-05-01
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| Series: | Engineering and Technology Journal |
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| Online Access: | https://etj.uotechnology.edu.iq/article_124094_7dd90560a9356cd5fb873d3f4e9d67ba.pdf |
| _version_ | 1797325850549420032 |
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| author | Ali Al - Sa'ady Dalal S. Bedan |
| author_facet | Ali Al - Sa'ady Dalal S. Bedan |
| author_sort | Ali Al - Sa'ady |
| collection | DOAJ |
| description | The optimum conditions for Ceruloplasmin (CP) activity have been measured in serum samples of a healthy individual without any detectable diseases. CP activity was evaluated by measurement of its p-phenylenediamine oxidase activity. This study showed the optimum pHforCP activity of a healthy human serum was 6.0 and the optimal temperature for CP activity was 45°C using in vitro conditions. The maximum value of oxidation activity was in incubation period70 min using optimal substrate concentration 60 mmol/l.Several types of antibiotics were experimented in order to detect their effect on ceruloplasmin oxidation activity; these are consisted of Cephalexin, Amoxicillin, Erythromycin, Ampicillin, Ampicillin & Cloxacillin, Streptomycin Sulfate, Chloramphenicol, Cefrtriaxone, Benzathine, Pencilline and Aspegic. Results showed that Ampicillin and Streptomycin Sulfate had maximum influence on ceruloplasmin activity. Different types of sugars, salts and anesthetics were used to evaluate their effect on p-phenylenediamine oxidation by serum ceruloplasmin. Results appeared that starch, FeSo4 and lidocaine 4% were activator for oxidation process. This study aimed to measure the optimal conditions of ceruloplasmin activity and to evaluate effect of different antibiotics and anesthetics onproteins from the serum of a healthy individual using in vitro conditions. |
| first_indexed | 2024-03-08T06:15:18Z |
| format | Article |
| id | doaj.art-2993a374ac9c4cb98198f21f48fe54de |
| institution | Directory Open Access Journal |
| issn | 1681-6900 2412-0758 |
| language | English |
| last_indexed | 2024-03-08T06:15:18Z |
| publishDate | 2016-05-01 |
| publisher | Unviversity of Technology- Iraq |
| record_format | Article |
| series | Engineering and Technology Journal |
| spelling | doaj.art-2993a374ac9c4cb98198f21f48fe54de2024-02-04T17:27:37ZengUnviversity of Technology- IraqEngineering and Technology Journal1681-69002412-07582016-05-01345B730737https://doi.org/10.30684/etj.34.5B.13124094Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitroAli Al - Sa'adyDalal S. BedanThe optimum conditions for Ceruloplasmin (CP) activity have been measured in serum samples of a healthy individual without any detectable diseases. CP activity was evaluated by measurement of its p-phenylenediamine oxidase activity. This study showed the optimum pHforCP activity of a healthy human serum was 6.0 and the optimal temperature for CP activity was 45°C using in vitro conditions. The maximum value of oxidation activity was in incubation period70 min using optimal substrate concentration 60 mmol/l.Several types of antibiotics were experimented in order to detect their effect on ceruloplasmin oxidation activity; these are consisted of Cephalexin, Amoxicillin, Erythromycin, Ampicillin, Ampicillin & Cloxacillin, Streptomycin Sulfate, Chloramphenicol, Cefrtriaxone, Benzathine, Pencilline and Aspegic. Results showed that Ampicillin and Streptomycin Sulfate had maximum influence on ceruloplasmin activity. Different types of sugars, salts and anesthetics were used to evaluate their effect on p-phenylenediamine oxidation by serum ceruloplasmin. Results appeared that starch, FeSo4 and lidocaine 4% were activator for oxidation process. This study aimed to measure the optimal conditions of ceruloplasmin activity and to evaluate effect of different antibiotics and anesthetics onproteins from the serum of a healthy individual using in vitro conditions.https://etj.uotechnology.edu.iq/article_124094_7dd90560a9356cd5fb873d3f4e9d67ba.pdfceruloplasminphenylenediamineantibioticsanestheticsoxidase activity |
| spellingShingle | Ali Al - Sa'ady Dalal S. Bedan Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro Engineering and Technology Journal ceruloplasmin phenylenediamine antibiotics anesthetics oxidase activity |
| title | Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro |
| title_full | Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro |
| title_fullStr | Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro |
| title_full_unstemmed | Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro |
| title_short | Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro |
| title_sort | effect of antibiotics anesthetics sugars and saltson the oxidation activity of human ceruloplasmin in vitro |
| topic | ceruloplasmin phenylenediamine antibiotics anesthetics oxidase activity |
| url | https://etj.uotechnology.edu.iq/article_124094_7dd90560a9356cd5fb873d3f4e9d67ba.pdf |
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