A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i>
Sequence type 59 (ST59) is the dominant type of community-associated methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) in Taiwan. Previously, we reported that ST59 MRSA harbors enterococcal IS<i>1216V</i>-mediated multidrug-resistant composite transposons MES<sub&g...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/1905 |
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| author | Yu-Tzu Lin Sung-Pin Tseng Wei-Wen Hung Chen-Chia Chang You-Han Chen Ya-Ting Jao Yen-Hsu Chen Lee-Jene Teng Wei-Chun Hung |
| author_facet | Yu-Tzu Lin Sung-Pin Tseng Wei-Wen Hung Chen-Chia Chang You-Han Chen Ya-Ting Jao Yen-Hsu Chen Lee-Jene Teng Wei-Chun Hung |
| author_sort | Yu-Tzu Lin |
| collection | DOAJ |
| description | Sequence type 59 (ST59) is the dominant type of community-associated methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) in Taiwan. Previously, we reported that ST59 MRSA harbors enterococcal IS<i>1216V</i>-mediated multidrug-resistant composite transposons MES<sub>PM1</sub> or MES<sub>6272-2</sub>. The MES were found to have a mosaic structure, largely originating in enterococci and partly native to <i>S. aureus</i>. The current study aimed to track the origin of the MES and how they disseminated from enterococci to ST59 <i>S. aureus</i>. A total of 270 enterococcal isolates were analyzed, showing that two ST64 <i>Enterococcus faecalis</i> isolated in 1992 and 11 clonal complex 17 <i>Enterococcus faecium</i> harbored MES<sub>PM1</sub>-like and MES<sub>6272-2</sub>-like structures, respectively. Sequence analysis revealed that ST64 <i>E. faecalis</i> strain N48 acquired the MES<sub>PM1</sub>-like structure on the plasmid pEflis48. The pEflis48 harbored the enterococci-originated region (erythromycin, kanamycin, and streptomycin resistances) and the <i>S.</i><i>aureus</i>-originated region (chloramphenicol resistance) of MES<sub>PM1</sub> but was separated by the replication region of the plasmid. Homologous recombination between the two direct repeats of IS<i>1216V</i> resulted in excision of the replication region of the plasmid to regenerate MES<sub>PM1</sub>. The p4780-1 and pV19 of <i>E. faecium</i> carried MES<sub>6272-2</sub>-like structures with IS<i>1216V</i>, albeit with multiple insertions by other insertion sequences. The findings show that IS<i>1216V</i> plays important roles in bidirectional gene transfer of multidrug resistance between enterococci and <i>S. aureus</i>. |
| first_indexed | 2024-03-10T14:25:59Z |
| format | Article |
| id | doaj.art-2994d72a88be4406abb30d7a3400793e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:25:59Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2994d72a88be4406abb30d7a3400793e2023-11-20T23:00:25ZengMDPI AGMicroorganisms2076-26072020-11-01812190510.3390/microorganisms8121905A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i>Yu-Tzu Lin0Sung-Pin Tseng1Wei-Wen Hung2Chen-Chia Chang3You-Han Chen4Ya-Ting Jao5Yen-Hsu Chen6Lee-Jene Teng7Wei-Chun Hung8Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404333, TaiwanDepartment of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung 807378, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807377, TaiwanDepartment of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, TaiwanDepartment of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, TaiwanInfection Control Center, Kaohsiung Medical University Hospital, Kaohsiung 807377, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807377, TaiwanDepartment of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei 100229, TaiwanDepartment of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, TaiwanSequence type 59 (ST59) is the dominant type of community-associated methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) in Taiwan. Previously, we reported that ST59 MRSA harbors enterococcal IS<i>1216V</i>-mediated multidrug-resistant composite transposons MES<sub>PM1</sub> or MES<sub>6272-2</sub>. The MES were found to have a mosaic structure, largely originating in enterococci and partly native to <i>S. aureus</i>. The current study aimed to track the origin of the MES and how they disseminated from enterococci to ST59 <i>S. aureus</i>. A total of 270 enterococcal isolates were analyzed, showing that two ST64 <i>Enterococcus faecalis</i> isolated in 1992 and 11 clonal complex 17 <i>Enterococcus faecium</i> harbored MES<sub>PM1</sub>-like and MES<sub>6272-2</sub>-like structures, respectively. Sequence analysis revealed that ST64 <i>E. faecalis</i> strain N48 acquired the MES<sub>PM1</sub>-like structure on the plasmid pEflis48. The pEflis48 harbored the enterococci-originated region (erythromycin, kanamycin, and streptomycin resistances) and the <i>S.</i><i>aureus</i>-originated region (chloramphenicol resistance) of MES<sub>PM1</sub> but was separated by the replication region of the plasmid. Homologous recombination between the two direct repeats of IS<i>1216V</i> resulted in excision of the replication region of the plasmid to regenerate MES<sub>PM1</sub>. The p4780-1 and pV19 of <i>E. faecium</i> carried MES<sub>6272-2</sub>-like structures with IS<i>1216V</i>, albeit with multiple insertions by other insertion sequences. The findings show that IS<i>1216V</i> plays important roles in bidirectional gene transfer of multidrug resistance between enterococci and <i>S. aureus</i>.https://www.mdpi.com/2076-2607/8/12/1905<i>Staphylococcus aureus</i>enterococciIS<i>1216V</i>gene transfer |
| spellingShingle | Yu-Tzu Lin Sung-Pin Tseng Wei-Wen Hung Chen-Chia Chang You-Han Chen Ya-Ting Jao Yen-Hsu Chen Lee-Jene Teng Wei-Chun Hung A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i> Microorganisms <i>Staphylococcus aureus</i> enterococci IS<i>1216V</i> gene transfer |
| title | A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i> |
| title_full | A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i> |
| title_fullStr | A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i> |
| title_full_unstemmed | A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i> |
| title_short | A Possible Role of Insertion Sequence IS<i>1216V</i> in Dissemination of Multidrug-Resistant Elements MES<sub>PM1</sub> and MES<sub>6272-2</sub> between <i>Enterococcus</i> and ST59 <i>Staphylococcus aureus</i> |
| title_sort | possible role of insertion sequence is i 1216v i in dissemination of multidrug resistant elements mes sub pm1 sub and mes sub 6272 2 sub between i enterococcus i and st59 i staphylococcus aureus i |
| topic | <i>Staphylococcus aureus</i> enterococci IS<i>1216V</i> gene transfer |
| url | https://www.mdpi.com/2076-2607/8/12/1905 |
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