Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide
Hispidulin is a natural bioactive flavonoid that has been studied for its potential therapeutic properties, including its anti-inflammatory, antioxidant, and neuroprotective effects. The aim of this study was to explore whether hispidulin could inhibit the endothelial inflammation triggered by <i...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/18/6717 |
| _version_ | 1827725043463880704 |
|---|---|
| author | Yeon Kim Hoyong Lee Hyun-Joo Park Mi-Kyoung Kim Yong-Il Kim Hyung Joon Kim Soo-Kyung Bae Yung-Jin Kim Moon-Kyoung Bae |
| author_facet | Yeon Kim Hoyong Lee Hyun-Joo Park Mi-Kyoung Kim Yong-Il Kim Hyung Joon Kim Soo-Kyung Bae Yung-Jin Kim Moon-Kyoung Bae |
| author_sort | Yeon Kim |
| collection | DOAJ |
| description | Hispidulin is a natural bioactive flavonoid that has been studied for its potential therapeutic properties, including its anti-inflammatory, antioxidant, and neuroprotective effects. The aim of this study was to explore whether hispidulin could inhibit the endothelial inflammation triggered by <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) lipopolysaccharide (LPS). The adhesion of monocytes to the vascular endothelium was evaluated through in vitro and ex vivo monocyte adhesion assays. We analyzed the migration of monocytes across the endothelial layer using a transmigration assay. The results showed that treatment with hispidulin decreased the <i>P. gingivalis</i> LPS-induced adhesion of monocytes to endothelial cells and their migration by suppressing the <i>P. gingivalis</i> LPS-triggered expression of intercellular adhesion molecule-1 (ICAM-1) through downregulating nuclear factor-қB (NF-қB). In addition, hispidulin inhibited <i>P. gingivalis</i> LPS-induced mitogen-activated protein kinases (MAPKs) and AKT in endothelial cells. Altogether, the results indicate that hispidulin suppresses the vascular inflammation induced by <i>P. gingivalis</i> LPS. Mechanistically, it prevents the adhesion of monocytes to the vascular endothelium and migration and inhibits NF-қB, MAPKs, and AKT signaling in endothelial cells. |
| first_indexed | 2024-03-10T22:23:00Z |
| format | Article |
| id | doaj.art-299a9d40454e4b50a6cf0fb898f61fa3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T22:23:00Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-299a9d40454e4b50a6cf0fb898f61fa32023-11-19T12:11:27ZengMDPI AGMolecules1420-30492023-09-012818671710.3390/molecules28186717Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> LipopolysaccharideYeon Kim0Hoyong Lee1Hyun-Joo Park2Mi-Kyoung Kim3Yong-Il Kim4Hyung Joon Kim5Soo-Kyung Bae6Yung-Jin Kim7Moon-Kyoung Bae8Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Molecular Biology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Orthodontics, School of Dentistry, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of KoreaDental and Life Science Institute, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Molecular Biology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of KoreaHispidulin is a natural bioactive flavonoid that has been studied for its potential therapeutic properties, including its anti-inflammatory, antioxidant, and neuroprotective effects. The aim of this study was to explore whether hispidulin could inhibit the endothelial inflammation triggered by <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) lipopolysaccharide (LPS). The adhesion of monocytes to the vascular endothelium was evaluated through in vitro and ex vivo monocyte adhesion assays. We analyzed the migration of monocytes across the endothelial layer using a transmigration assay. The results showed that treatment with hispidulin decreased the <i>P. gingivalis</i> LPS-induced adhesion of monocytes to endothelial cells and their migration by suppressing the <i>P. gingivalis</i> LPS-triggered expression of intercellular adhesion molecule-1 (ICAM-1) through downregulating nuclear factor-қB (NF-қB). In addition, hispidulin inhibited <i>P. gingivalis</i> LPS-induced mitogen-activated protein kinases (MAPKs) and AKT in endothelial cells. Altogether, the results indicate that hispidulin suppresses the vascular inflammation induced by <i>P. gingivalis</i> LPS. Mechanistically, it prevents the adhesion of monocytes to the vascular endothelium and migration and inhibits NF-қB, MAPKs, and AKT signaling in endothelial cells.https://www.mdpi.com/1420-3049/28/18/6717hispidulin<i>Porphyromonas gingivalis</i>lipopolysaccharidemonocytevascular endothelial cellsvascular inflammation |
| spellingShingle | Yeon Kim Hoyong Lee Hyun-Joo Park Mi-Kyoung Kim Yong-Il Kim Hyung Joon Kim Soo-Kyung Bae Yung-Jin Kim Moon-Kyoung Bae Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide Molecules hispidulin <i>Porphyromonas gingivalis</i> lipopolysaccharide monocyte vascular endothelial cells vascular inflammation |
| title | Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide |
| title_full | Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide |
| title_fullStr | Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide |
| title_full_unstemmed | Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide |
| title_short | Hispidulin Inhibits the Vascular Inflammation Triggered by <i>Porphyromonas gingivalis</i> Lipopolysaccharide |
| title_sort | hispidulin inhibits the vascular inflammation triggered by i porphyromonas gingivalis i lipopolysaccharide |
| topic | hispidulin <i>Porphyromonas gingivalis</i> lipopolysaccharide monocyte vascular endothelial cells vascular inflammation |
| url | https://www.mdpi.com/1420-3049/28/18/6717 |
| work_keys_str_mv | AT yeonkim hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT hoyonglee hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT hyunjoopark hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT mikyoungkim hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT yongilkim hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT hyungjoonkim hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT sookyungbae hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT yungjinkim hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide AT moonkyoungbae hispidulininhibitsthevascularinflammationtriggeredbyiporphyromonasgingivalisilipopolysaccharide |