Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato
Abstract Background bHLH transcription factors play significant roles in regulating plant growth and development, stress response, and anthocyanin biosynthesis. Sweetpotato is a pivotal food and industry crop, but little information is available on sweetpotato bHLH genes. Results Herein, 227 putativ...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-03-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-04788-0 |
| _version_ | 1797275267660513280 |
|---|---|
| author | Fen Guo Xiaoqing Meng Haiting Hong Siyuan Liu Jing Yu Can Huang Tingting Dong Huixue Geng Zongyun Li Mingku Zhu |
| author_facet | Fen Guo Xiaoqing Meng Haiting Hong Siyuan Liu Jing Yu Can Huang Tingting Dong Huixue Geng Zongyun Li Mingku Zhu |
| author_sort | Fen Guo |
| collection | DOAJ |
| description | Abstract Background bHLH transcription factors play significant roles in regulating plant growth and development, stress response, and anthocyanin biosynthesis. Sweetpotato is a pivotal food and industry crop, but little information is available on sweetpotato bHLH genes. Results Herein, 227 putative IbbHLH genes were defined on sweetpotato chromosomes, and fragment duplications were identified as the dominant driving force for IbbHLH expansion. These IbbHLHs were divided into 26 subfamilies through phylogenetic analysis, as supported by further analysis of exon-intron structure and conserved motif composition. The syntenic analysis between IbbHLHs and their orthologs from other plants depicted evolutionary relationships of IbbHLHs. Based on the transcriptome data under salt stress, the expression of 12 IbbHLHs was screened for validation by qRT-PCR, and differential and significant transcriptions under abiotic stress were detected. Moreover, IbbHLH123 and IbbHLH215, which were remarkably upregulated by stress treatments, had obvious transactivation activity in yeasts. Protein interaction detections and yeast two-hybrid assays suggested an intricate interaction correlation between IbbHLHs. Besides, transcriptome screening revealed that multiple IbbHLHs may be closely related to anthocyanin biosynthesis based on the phenotype (purple vs. white tissues), which was confirmed by subsequent qRT-PCR analysis. Conclusions These results shed light on the promising functions of sweetpotato IbbHLHs in abiotic stress response and anthocyanin biosynthesis. |
| first_indexed | 2024-03-07T15:10:50Z |
| format | Article |
| id | doaj.art-299ef349c07943068d71150ae3b759b4 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:10:50Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-299ef349c07943068d71150ae3b759b42024-03-05T18:38:28ZengBMCBMC Plant Biology1471-22292024-03-0124111410.1186/s12870-024-04788-0Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotatoFen Guo0Xiaoqing Meng1Haiting Hong2Siyuan Liu3Jing Yu4Can Huang5Tingting Dong6Huixue Geng7Zongyun Li8Mingku Zhu9Institute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityInstitute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal UniversityAbstract Background bHLH transcription factors play significant roles in regulating plant growth and development, stress response, and anthocyanin biosynthesis. Sweetpotato is a pivotal food and industry crop, but little information is available on sweetpotato bHLH genes. Results Herein, 227 putative IbbHLH genes were defined on sweetpotato chromosomes, and fragment duplications were identified as the dominant driving force for IbbHLH expansion. These IbbHLHs were divided into 26 subfamilies through phylogenetic analysis, as supported by further analysis of exon-intron structure and conserved motif composition. The syntenic analysis between IbbHLHs and their orthologs from other plants depicted evolutionary relationships of IbbHLHs. Based on the transcriptome data under salt stress, the expression of 12 IbbHLHs was screened for validation by qRT-PCR, and differential and significant transcriptions under abiotic stress were detected. Moreover, IbbHLH123 and IbbHLH215, which were remarkably upregulated by stress treatments, had obvious transactivation activity in yeasts. Protein interaction detections and yeast two-hybrid assays suggested an intricate interaction correlation between IbbHLHs. Besides, transcriptome screening revealed that multiple IbbHLHs may be closely related to anthocyanin biosynthesis based on the phenotype (purple vs. white tissues), which was confirmed by subsequent qRT-PCR analysis. Conclusions These results shed light on the promising functions of sweetpotato IbbHLHs in abiotic stress response and anthocyanin biosynthesis.https://doi.org/10.1186/s12870-024-04788-0Abiotic stressAnthocyanin biosynthesisbHLH transcription factorExpression profileSweetpotato |
| spellingShingle | Fen Guo Xiaoqing Meng Haiting Hong Siyuan Liu Jing Yu Can Huang Tingting Dong Huixue Geng Zongyun Li Mingku Zhu Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato BMC Plant Biology Abiotic stress Anthocyanin biosynthesis bHLH transcription factor Expression profile Sweetpotato |
| title | Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato |
| title_full | Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato |
| title_fullStr | Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato |
| title_full_unstemmed | Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato |
| title_short | Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato |
| title_sort | systematic identification and expression analysis of bhlh gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato |
| topic | Abiotic stress Anthocyanin biosynthesis bHLH transcription factor Expression profile Sweetpotato |
| url | https://doi.org/10.1186/s12870-024-04788-0 |
| work_keys_str_mv | AT fenguo systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT xiaoqingmeng systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT haitinghong systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT siyuanliu systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT jingyu systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT canhuang systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT tingtingdong systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT huixuegeng systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT zongyunli systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato AT mingkuzhu systematicidentificationandexpressionanalysisofbhlhgenefamilyrevealtheirrelevancetoabioticstressresponseandanthocyaninbiosynthesisinsweetpotato |