Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea
A high-performance liquid chromatography-ultraviolet detector (HPLC-UV) method has been used to quantify teicoplanin concentrations in human plasma. However, the limited analytical accuracy of previously bioanalytical methods for teicoplanin has given rise to uncertainty due to the use of an externa...
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MDPI AG
2021-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/4/572 |
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| author | Jaeok Lee Eun-Kyoung Chung Sung-Wook Kang Hwa-Jeong Lee Sandy-Jeong Rhie |
| author_facet | Jaeok Lee Eun-Kyoung Chung Sung-Wook Kang Hwa-Jeong Lee Sandy-Jeong Rhie |
| author_sort | Jaeok Lee |
| collection | DOAJ |
| description | A high-performance liquid chromatography-ultraviolet detector (HPLC-UV) method has been used to quantify teicoplanin concentrations in human plasma. However, the limited analytical accuracy of previously bioanalytical methods for teicoplanin has given rise to uncertainty due to the use of an external standard. In this study, an internal standard (IS), polymyxin B, was applied to devise a precise, accurate, and feasible HPLC-UV method. The deproteinized plasma sample containing teicoplanin and an IS of acetonitrile was chromatographed on a C18 column with an acidic mobile phase consisting of NaH<sub>2</sub>PO<sub>4</sub> buffer and acetonitrile (78:22, <i>v</i>/<i>v</i>) by isocratic elution and detection at 220 nm. The linearity was in the range 7.8–500 mg/L calculated by the ratio of the teicoplanin signal to the IS signal. This analytical method, validated by FDA guidelines with ICH Q2 (R1), was successfully applied to analyze the plasma samples of patients in the intensive care unit for treating serious resistant bacterial infectious diseases, such as those by methicillin-resistant <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i>. The methods suggested the potential for use in routine clinical practice for therapeutic drug monitoring of teicoplanin, providing both improved accuracy and a wide range of linearity from lower than steady-state trough concentrations (10 mg/L) to much higher concentrations. |
| first_indexed | 2024-03-10T12:14:11Z |
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| id | doaj.art-29a05529f3fb4486aacedf53db9e4233 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T12:14:11Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-29a05529f3fb4486aacedf53db9e42332023-11-21T15:58:33ZengMDPI AGPharmaceutics1999-49232021-04-0113457210.3390/pharmaceutics13040572Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in KoreaJaeok Lee0Eun-Kyoung Chung1Sung-Wook Kang2Hwa-Jeong Lee3Sandy-Jeong Rhie4College of Pharmacy and Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, KoreaDepartment of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 02453, KoreaDepartment of Pulmonary, Allergy and Critical Care Medicine, Kyung Hee University Hospital at Gangdong, School of Medicine, Kyung Hee University, Seoul 05278, KoreaCollege of Pharmacy and Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, KoreaCollege of Pharmacy and Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, KoreaA high-performance liquid chromatography-ultraviolet detector (HPLC-UV) method has been used to quantify teicoplanin concentrations in human plasma. However, the limited analytical accuracy of previously bioanalytical methods for teicoplanin has given rise to uncertainty due to the use of an external standard. In this study, an internal standard (IS), polymyxin B, was applied to devise a precise, accurate, and feasible HPLC-UV method. The deproteinized plasma sample containing teicoplanin and an IS of acetonitrile was chromatographed on a C18 column with an acidic mobile phase consisting of NaH<sub>2</sub>PO<sub>4</sub> buffer and acetonitrile (78:22, <i>v</i>/<i>v</i>) by isocratic elution and detection at 220 nm. The linearity was in the range 7.8–500 mg/L calculated by the ratio of the teicoplanin signal to the IS signal. This analytical method, validated by FDA guidelines with ICH Q2 (R1), was successfully applied to analyze the plasma samples of patients in the intensive care unit for treating serious resistant bacterial infectious diseases, such as those by methicillin-resistant <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i>. The methods suggested the potential for use in routine clinical practice for therapeutic drug monitoring of teicoplanin, providing both improved accuracy and a wide range of linearity from lower than steady-state trough concentrations (10 mg/L) to much higher concentrations.https://www.mdpi.com/1999-4923/13/4/572teicoplaninpolymyxin BHPLC-UVinternal standardhuman plasmaclinical application |
| spellingShingle | Jaeok Lee Eun-Kyoung Chung Sung-Wook Kang Hwa-Jeong Lee Sandy-Jeong Rhie Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea Pharmaceutics teicoplanin polymyxin B HPLC-UV internal standard human plasma clinical application |
| title | Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea |
| title_full | Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea |
| title_fullStr | Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea |
| title_full_unstemmed | Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea |
| title_short | Quantification of Teicoplanin Using the HPLC-UV Method for Clinical Applications in Critically Ill Patients in Korea |
| title_sort | quantification of teicoplanin using the hplc uv method for clinical applications in critically ill patients in korea |
| topic | teicoplanin polymyxin B HPLC-UV internal standard human plasma clinical application |
| url | https://www.mdpi.com/1999-4923/13/4/572 |
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