Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER
The recombinant surface antigen of hepatitis B virus (HBsAg), purified from transgenic plants, proved to be efficient when utilized for raising anti-HB antibodies for the prevention of hepatitis B. Because of the important role of the HBsAg antigen in hepatitis B prevention, the coding sequence of H...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Catarina
2010-03-01
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| Series: | Biotemas |
| Subjects: | |
| Online Access: | http://www.biotemas.ufsc.br/volumes/pdf/volume231/1a11.pdf |
| _version_ | 1811303244654182400 |
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| author | Juliana Martins Ribeiro Débora Costa Bastos Eduardo Alves Gamosa de Oliveira Jackson Antônio Marcondes de Souza Márcio dos Santos Teixeira Pinto Ekkehard Ernst Theodor Hansen |
| author_facet | Juliana Martins Ribeiro Débora Costa Bastos Eduardo Alves Gamosa de Oliveira Jackson Antônio Marcondes de Souza Márcio dos Santos Teixeira Pinto Ekkehard Ernst Theodor Hansen |
| author_sort | Juliana Martins Ribeiro |
| collection | DOAJ |
| description | The recombinant surface antigen of hepatitis B virus (HBsAg), purified from transgenic plants, proved to be efficient when utilized for raising anti-HB antibodies for the prevention of hepatitis B. Because of the important role of the HBsAg antigen in hepatitis B prevention, the coding sequence of HBsAg antigen, with or without the addition of the carboxi-terminus sequence for protein retention in the endoplasmatic reticulum, was linked to cauliflower mosaic virus 35S promoter, tobacco mosaic virus leader sequence Ω, and the transcription terminator sequence. The aim of this work was to clone the chimeric gene 35SHBsAgER in the plant expression vector pGPTV/Kan/Asc. The resulting plasmid, called pG35SHBsAgER, and another plasmid produced previously in our laboratory called pG35SHBsAg, were transferred to Agrobacterium tumefaciens, and tobacco leaves, of the SR1 cultivar were used as explants for genetic transformation. Twenty-one fully regenerated plants were obtained (10 for the pG35SHBsAg construction and 11 for the pG35SHBsAgER construction). The genomic DNA of all plants was analyzed by PCR, and the presence of the transgene was confirmed in all plants. |
| first_indexed | 2024-04-13T07:44:34Z |
| format | Article |
| id | doaj.art-29a173df92004dd088f394b68712294d |
| institution | Directory Open Access Journal |
| issn | 0103-1643 |
| language | English |
| last_indexed | 2024-04-13T07:44:34Z |
| publishDate | 2010-03-01 |
| publisher | Universidade Federal de Santa Catarina |
| record_format | Article |
| series | Biotemas |
| spelling | doaj.art-29a173df92004dd088f394b68712294d2022-12-22T02:55:44ZengUniversidade Federal de Santa CatarinaBiotemas0103-16432010-03-01231111Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgERJuliana Martins RibeiroDébora Costa BastosEduardo Alves Gamosa de OliveiraJackson Antônio Marcondes de SouzaMárcio dos Santos Teixeira PintoEkkehard Ernst Theodor HansenThe recombinant surface antigen of hepatitis B virus (HBsAg), purified from transgenic plants, proved to be efficient when utilized for raising anti-HB antibodies for the prevention of hepatitis B. Because of the important role of the HBsAg antigen in hepatitis B prevention, the coding sequence of HBsAg antigen, with or without the addition of the carboxi-terminus sequence for protein retention in the endoplasmatic reticulum, was linked to cauliflower mosaic virus 35S promoter, tobacco mosaic virus leader sequence Ω, and the transcription terminator sequence. The aim of this work was to clone the chimeric gene 35SHBsAgER in the plant expression vector pGPTV/Kan/Asc. The resulting plasmid, called pG35SHBsAgER, and another plasmid produced previously in our laboratory called pG35SHBsAg, were transferred to Agrobacterium tumefaciens, and tobacco leaves, of the SR1 cultivar were used as explants for genetic transformation. Twenty-one fully regenerated plants were obtained (10 for the pG35SHBsAg construction and 11 for the pG35SHBsAgER construction). The genomic DNA of all plants was analyzed by PCR, and the presence of the transgene was confirmed in all plants.http://www.biotemas.ufsc.br/volumes/pdf/volume231/1a11.pdfendoplasmatic reticulum retention sequenceHBsAg antigenplants as bioreactors |
| spellingShingle | Juliana Martins Ribeiro Débora Costa Bastos Eduardo Alves Gamosa de Oliveira Jackson Antônio Marcondes de Souza Márcio dos Santos Teixeira Pinto Ekkehard Ernst Theodor Hansen Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER Biotemas endoplasmatic reticulum retention sequence HBsAg antigen plants as bioreactors |
| title | Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER |
| title_full | Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER |
| title_fullStr | Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER |
| title_full_unstemmed | Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER |
| title_short | Transformation of tobacco plant (Nicotiana tabacum L.) with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER |
| title_sort | transformation of tobacco plant nicotiana tabacum l with the recombinant hepatitis b virus genes 35shbsag and 35shbsager |
| topic | endoplasmatic reticulum retention sequence HBsAg antigen plants as bioreactors |
| url | http://www.biotemas.ufsc.br/volumes/pdf/volume231/1a11.pdf |
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