Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy
Adenomyosis is a common gynaecological disease associated with the presence of endometrial lesions in the uterine myometrium. Estrogens have been proven to be the crucial hormones driving the growth of adenomyosis. Little is known about the distinct mechanisms of progesterone action in adenomyosis....
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MDPI AG
2022-07-01
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| Series: | Journal of Clinical Medicine |
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| author | Maria Sztachelska Donata Ponikwicka-Tyszko Lydia Martínez-Rodrigo Piotr Bernaczyk Ewelina Palak Weronika Półchłopek Tomasz Bielawski Sławomir Wołczyński |
| author_facet | Maria Sztachelska Donata Ponikwicka-Tyszko Lydia Martínez-Rodrigo Piotr Bernaczyk Ewelina Palak Weronika Półchłopek Tomasz Bielawski Sławomir Wołczyński |
| author_sort | Maria Sztachelska |
| collection | DOAJ |
| description | Adenomyosis is a common gynaecological disease associated with the presence of endometrial lesions in the uterine myometrium. Estrogens have been proven to be the crucial hormones driving the growth of adenomyosis. Little is known about the distinct mechanisms of progesterone action in adenomyosis. Hence, in this study, we decided to characterize the expression of all nuclear and membrane estrogen and progesterone receptors. Additionally, as a functional investigation, we monitored prolactin production and cell proliferation after estradiol and progesterone treatments. We confirmed the presence of all nuclear and membrane estrogen and progesterone receptors in adenomyotic lesions at gene and protein levels. The expression of membrane progesterone receptors α and β (mPRα, mPRβ) as well as estrogen receptor β (ERβ) was upregulated in adenomyosis compared to normal myometrium. Estradiol significantly increased adenomyotic cell proliferation. Progesterone and cAMP upregulated prolactin secretion in adenomyosis in the same pattern as in the normal endometrium. In the present study, we showed the functional link between estradiol action and adenomyotic cell proliferation, as well as progesterone and prolactin production. Our findings provide novel insights into the sex steroid receptor expression pattern and potential regulated pathways in adenomyosis, suggesting that all receptors play an important role in adenomyosis pathophysiology. |
| first_indexed | 2024-03-09T12:27:29Z |
| format | Article |
| id | doaj.art-29a56709b71c4a5da7f84264d569b328 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T12:27:29Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-29a56709b71c4a5da7f84264d569b3282023-11-30T22:32:56ZengMDPI AGJournal of Clinical Medicine2077-03832022-07-011115440710.3390/jcm11154407Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological TherapyMaria Sztachelska0Donata Ponikwicka-Tyszko1Lydia Martínez-Rodrigo2Piotr Bernaczyk3Ewelina Palak4Weronika Półchłopek5Tomasz Bielawski6Sławomir Wołczyński7Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Pathomorphology, Medical University of Bialystok, 15-269 Białystok, PolandDepartment of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, 15-276 Białystok, PolandDepartment of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandAdenomyosis is a common gynaecological disease associated with the presence of endometrial lesions in the uterine myometrium. Estrogens have been proven to be the crucial hormones driving the growth of adenomyosis. Little is known about the distinct mechanisms of progesterone action in adenomyosis. Hence, in this study, we decided to characterize the expression of all nuclear and membrane estrogen and progesterone receptors. Additionally, as a functional investigation, we monitored prolactin production and cell proliferation after estradiol and progesterone treatments. We confirmed the presence of all nuclear and membrane estrogen and progesterone receptors in adenomyotic lesions at gene and protein levels. The expression of membrane progesterone receptors α and β (mPRα, mPRβ) as well as estrogen receptor β (ERβ) was upregulated in adenomyosis compared to normal myometrium. Estradiol significantly increased adenomyotic cell proliferation. Progesterone and cAMP upregulated prolactin secretion in adenomyosis in the same pattern as in the normal endometrium. In the present study, we showed the functional link between estradiol action and adenomyotic cell proliferation, as well as progesterone and prolactin production. Our findings provide novel insights into the sex steroid receptor expression pattern and potential regulated pathways in adenomyosis, suggesting that all receptors play an important role in adenomyosis pathophysiology.https://www.mdpi.com/2077-0383/11/15/4407adenomyosissex steroidsestrogen receptorsprogesterone receptorsaromataseprolactin |
| spellingShingle | Maria Sztachelska Donata Ponikwicka-Tyszko Lydia Martínez-Rodrigo Piotr Bernaczyk Ewelina Palak Weronika Półchłopek Tomasz Bielawski Sławomir Wołczyński Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy Journal of Clinical Medicine adenomyosis sex steroids estrogen receptors progesterone receptors aromatase prolactin |
| title | Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy |
| title_full | Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy |
| title_fullStr | Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy |
| title_full_unstemmed | Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy |
| title_short | Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy |
| title_sort | functional implications of estrogen and progesterone receptors expression in adenomyosis potential targets for endocrinological therapy |
| topic | adenomyosis sex steroids estrogen receptors progesterone receptors aromatase prolactin |
| url | https://www.mdpi.com/2077-0383/11/15/4407 |
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