Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles
Vascular endothelial function deteriorates with age and disease, and the production of vasodilator factors like nitric oxide (NO) decreases. The free amino acid l-citrulline increases vasodilation and blood flow through increased NO production. We examined the effects of oral l-citrulline administra...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821002430 |
| _version_ | 1819101937625202688 |
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| author | Sho Miyatake Kazuo Hino Goro Ebisu Satoshi Fujita |
| author_facet | Sho Miyatake Kazuo Hino Goro Ebisu Satoshi Fujita |
| author_sort | Sho Miyatake |
| collection | DOAJ |
| description | Vascular endothelial function deteriorates with age and disease, and the production of vasodilator factors like nitric oxide (NO) decreases. The free amino acid l-citrulline increases vasodilation and blood flow through increased NO production. We examined the effects of oral l-citrulline administration on vascular delivery of substances to skeletal muscles. In Experiment 1, following oral l-citrulline administration and subsequent intravenous Evans blue dye (EBD) administration to rats, EBD levels delivered to skeletal muscles were measured after 60 min. In Experiment 2, plasma concentrations of amino acids and NOx, an indicator of vasodilation, were measured over time after oral l-citrulline administration. In Experiment 3, we measured EBD levels in skeletal muscles of streptozotocin-induced type 1 diabetic rats following l-citrulline administration. In these experiments, EBD levels in the soleus muscle were higher in the l-citrulline group than in the control group (19.9 ± 0.7 vs. 22.5 ± 1.9 μg/g tissue, p < 0.05). Plasma l-arginine, l-citrulline, and NOx levels were increased within 30 min after l-citrulline administration. EBD levels in the soleus and gastrocnemius muscles were higher in diabetic rats with l-citrulline administration (18.7 ± 2.2 vs. 25.0 ± 4.3 μg/g tissue, p < 0.05 and 8.0 ± 0.5 vs. 9.2 ± 0.8 μg/g tissue, p = 0.05, respectively). These data suggest that oral l-citrulline administration may increase the level of substances delivered to skeletal muscles by increasing the NO production in both normal and vascular endothelial dysfunction models. |
| first_indexed | 2024-12-22T01:26:36Z |
| format | Article |
| id | doaj.art-29a979d539bc4ccca1e7253ccbfb8d7a |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-22T01:26:36Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-29a979d539bc4ccca1e7253ccbfb8d7a2022-12-21T18:43:35ZengElsevierBiochemistry and Biophysics Reports2405-58082021-12-0128101149Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal musclesSho Miyatake0Kazuo Hino1Goro Ebisu2Satoshi Fujita3OS-1 Division, Medical Foods Research Institute, Otsuka Pharmaceutical Factory, Inc., 115 Kuguhara, Tateiwa, Muya-cho, Naruto, Tokushima, 772-8601, Japan; Faculty of Sport and Health Science, Ritsumeikan University, 1-1-1, Nojihigashi, Kusatsu, Shiga, 525-8577, JapanOS-1 Division, Medical Foods Research Institute, Otsuka Pharmaceutical Factory, Inc., 115 Kuguhara, Tateiwa, Muya-cho, Naruto, Tokushima, 772-8601, JapanOS-1 Division, Medical Foods Research Institute, Otsuka Pharmaceutical Factory, Inc., 115 Kuguhara, Tateiwa, Muya-cho, Naruto, Tokushima, 772-8601, JapanFaculty of Sport and Health Science, Ritsumeikan University, 1-1-1, Nojihigashi, Kusatsu, Shiga, 525-8577, Japan; Corresponding author.Vascular endothelial function deteriorates with age and disease, and the production of vasodilator factors like nitric oxide (NO) decreases. The free amino acid l-citrulline increases vasodilation and blood flow through increased NO production. We examined the effects of oral l-citrulline administration on vascular delivery of substances to skeletal muscles. In Experiment 1, following oral l-citrulline administration and subsequent intravenous Evans blue dye (EBD) administration to rats, EBD levels delivered to skeletal muscles were measured after 60 min. In Experiment 2, plasma concentrations of amino acids and NOx, an indicator of vasodilation, were measured over time after oral l-citrulline administration. In Experiment 3, we measured EBD levels in skeletal muscles of streptozotocin-induced type 1 diabetic rats following l-citrulline administration. In these experiments, EBD levels in the soleus muscle were higher in the l-citrulline group than in the control group (19.9 ± 0.7 vs. 22.5 ± 1.9 μg/g tissue, p < 0.05). Plasma l-arginine, l-citrulline, and NOx levels were increased within 30 min after l-citrulline administration. EBD levels in the soleus and gastrocnemius muscles were higher in diabetic rats with l-citrulline administration (18.7 ± 2.2 vs. 25.0 ± 4.3 μg/g tissue, p < 0.05 and 8.0 ± 0.5 vs. 9.2 ± 0.8 μg/g tissue, p = 0.05, respectively). These data suggest that oral l-citrulline administration may increase the level of substances delivered to skeletal muscles by increasing the NO production in both normal and vascular endothelial dysfunction models.http://www.sciencedirect.com/science/article/pii/S2405580821002430l-citrullineSkeletal muscleVascular endotheliumDiabetesNitric oxide |
| spellingShingle | Sho Miyatake Kazuo Hino Goro Ebisu Satoshi Fujita Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles Biochemistry and Biophysics Reports l-citrulline Skeletal muscle Vascular endothelium Diabetes Nitric oxide |
| title | Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles |
| title_full | Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles |
| title_fullStr | Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles |
| title_full_unstemmed | Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles |
| title_short | Oral administration of l-citrulline alters the vascular delivery of substances to rat skeletal muscles |
| title_sort | oral administration of l citrulline alters the vascular delivery of substances to rat skeletal muscles |
| topic | l-citrulline Skeletal muscle Vascular endothelium Diabetes Nitric oxide |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821002430 |
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