Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves
This study was conducted to investigate the effect of HS on HSPs gene expression in bovine PBMCs of beef calves in in vitro and in vivo models. In the in vitro experiment, blood samples were collected from the jugular vein of five beef calves (age: 174.2 ± 5.20 days, BW: 145.2 ± 5.21 kg). In the in...
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MDPI AG
2020-05-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/5/895 |
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| author | Won-Seob Kim Jalil Ghassemi Nejad Sang-Gun Roh Hong-Gu Lee |
| author_facet | Won-Seob Kim Jalil Ghassemi Nejad Sang-Gun Roh Hong-Gu Lee |
| author_sort | Won-Seob Kim |
| collection | DOAJ |
| description | This study was conducted to investigate the effect of HS on HSPs gene expression in bovine PBMCs of beef calves in in vitro and in vivo models. In the in vitro experiment, blood samples were collected from the jugular vein of five beef calves (age: 174.2 ± 5.20 days, BW: 145.2 ± 5.21 kg). In the in vivo experiment, sixteen Korean native male beef calves (age: 169.6 ± 4.60 days, BW: 136.9 ± 6.23 kg) were exposed to ambient temperature for seven days (22 to 24 °C, relative humidity 60%; temperature–humidity index (THI) = 68 to 70) and subsequently to the temperature and humidity corresponding to the target THI level for 21 days (HS). For PBMC isolation, blood samples were collected every three days. In the in vitro model, the cell viability was significantly decreased in HS groups compared with the control group (<i>p</i> = 0.015). The expression of HSP70 (<i>p</i> = 0.022), HSP90 (<i>p</i> = 0.003) and HSPB1 (<i>p</i> = 0.026) genes was increased in the HS group in in vitro model. In the in vivo experiment, the HSP70 gene expression was increased after sudden exposure to HS conditions (severe THI levels; THI = 88 to 90), whereas HSP90 and HSPB1 showed no differences among the THI groups (<i>p</i> > 0.05). However, in the severe THI group, the HSP70 gene expression returned to normal range after six days of continuous HS. In conclusion, the HSP70 gene plays a pivotal role in protecting cells from damage and is sensitive to HS in immune cells compared with other HSP genes in in vitro and in vivo models. In addition, the in vivo models suggest that calves exhibit active physiological mechanisms of adaptation to HS after six days of continuous exposure by regulating the HSP70 gene expression. |
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| language | English |
| last_indexed | 2024-03-10T19:41:32Z |
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| spelling | doaj.art-29af567be71347218c3fa729792395832023-11-20T01:11:41ZengMDPI AGAnimals2076-26152020-05-0110589510.3390/ani10050895Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef CalvesWon-Seob Kim0Jalil Ghassemi Nejad1Sang-Gun Roh2Hong-Gu Lee3Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaGraduate School of Agricultural Science, Tohoku University, Sendai 980-8577, JapanDepartment of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaThis study was conducted to investigate the effect of HS on HSPs gene expression in bovine PBMCs of beef calves in in vitro and in vivo models. In the in vitro experiment, blood samples were collected from the jugular vein of five beef calves (age: 174.2 ± 5.20 days, BW: 145.2 ± 5.21 kg). In the in vivo experiment, sixteen Korean native male beef calves (age: 169.6 ± 4.60 days, BW: 136.9 ± 6.23 kg) were exposed to ambient temperature for seven days (22 to 24 °C, relative humidity 60%; temperature–humidity index (THI) = 68 to 70) and subsequently to the temperature and humidity corresponding to the target THI level for 21 days (HS). For PBMC isolation, blood samples were collected every three days. In the in vitro model, the cell viability was significantly decreased in HS groups compared with the control group (<i>p</i> = 0.015). The expression of HSP70 (<i>p</i> = 0.022), HSP90 (<i>p</i> = 0.003) and HSPB1 (<i>p</i> = 0.026) genes was increased in the HS group in in vitro model. In the in vivo experiment, the HSP70 gene expression was increased after sudden exposure to HS conditions (severe THI levels; THI = 88 to 90), whereas HSP90 and HSPB1 showed no differences among the THI groups (<i>p</i> > 0.05). However, in the severe THI group, the HSP70 gene expression returned to normal range after six days of continuous HS. In conclusion, the HSP70 gene plays a pivotal role in protecting cells from damage and is sensitive to HS in immune cells compared with other HSP genes in in vitro and in vivo models. In addition, the in vivo models suggest that calves exhibit active physiological mechanisms of adaptation to HS after six days of continuous exposure by regulating the HSP70 gene expression.https://www.mdpi.com/2076-2615/10/5/895beef calfheat-shock proteinsheat stressPBMCs |
| spellingShingle | Won-Seob Kim Jalil Ghassemi Nejad Sang-Gun Roh Hong-Gu Lee Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves Animals beef calf heat-shock proteins heat stress PBMCs |
| title | Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves |
| title_full | Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves |
| title_fullStr | Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves |
| title_full_unstemmed | Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves |
| title_short | Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves |
| title_sort | heat shock proteins gene expression in peripheral blood mononuclear cells as an indicator of heat stress in beef calves |
| topic | beef calf heat-shock proteins heat stress PBMCs |
| url | https://www.mdpi.com/2076-2615/10/5/895 |
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