Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)
One special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor t...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2019-09-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3050 |
| _version_ | 1828778740125007872 |
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| author | Yue Zhang Ao Zhang Yue Zhao Xiaohang Feng Yuyao Sheng Haolin Zhang Qiang Weng Meiyu Xu |
| author_facet | Yue Zhang Ao Zhang Yue Zhao Xiaohang Feng Yuyao Sheng Haolin Zhang Qiang Weng Meiyu Xu |
| author_sort | Yue Zhang |
| collection | DOAJ |
| description | One special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor type I (IL1R1) proteins were expressed in the oviduct of Rana dybowskii. In this study, we continued to investigate the localizations and expression levels of cytokines including toll-like receptor 4 (TLR4) and its related factors, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase (IRAK) and nuclear factor kappa B (NF-κΒ) in the oviduct of Rana dybowskii during the breeding period and pre-hibernation. Morphological results showed that there were significant differences in oviductal weight and pipe diameter with significantly higher values in pre-hibernation than those of the breeding period. Strong immunostaining of TLR4, MyD88, IRAK4 and NF-κΒ were observed in the pre-hibernation. These proteins levels had no significant difference between these two periods except MyD88 which was significantly higher in the pre-hibernation. The mRNA expression levels of MyD88 and NF-κΒ were obviously higher in pre-hibernation than those of the breeding period. These findings suggested that TLR4/MyD88 signal pathway might participate in the oviductal functions of Rana dybowskii during the breeding period and pre-hibernation. |
| first_indexed | 2024-12-11T16:48:25Z |
| format | Article |
| id | doaj.art-29aff51daa90493191ea4a90708ca328 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T16:48:25Z |
| publishDate | 2019-09-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-29aff51daa90493191ea4a90708ca3282022-12-22T00:58:09ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062019-09-0163310.4081/ejh.2019.3050Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)Yue Zhang0Ao Zhang1Yue Zhao2Xiaohang Feng3Yuyao Sheng4Haolin Zhang5Qiang Weng6Meiyu Xu7College of Biological Science and Technology, Beijing Forestry University, Beijing College of Biological Science and Technology, Beijing Forestry University, BeijingCollege of Biological Science and Technology, Beijing Forestry University, BeijingCollege of Biological Science and Technology, Beijing Forestry University, BeijingCollege of Biological Science and Technology, Beijing Forestry University, BeijingCollege of Biological Science and Technology, Beijing Forestry University, BeijingCollege of Biological Science and Technology, Beijing Forestry University, BeijingCollege of Biological Science and Technology, Beijing Forestry University, BeijingOne special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor type I (IL1R1) proteins were expressed in the oviduct of Rana dybowskii. In this study, we continued to investigate the localizations and expression levels of cytokines including toll-like receptor 4 (TLR4) and its related factors, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase (IRAK) and nuclear factor kappa B (NF-κΒ) in the oviduct of Rana dybowskii during the breeding period and pre-hibernation. Morphological results showed that there were significant differences in oviductal weight and pipe diameter with significantly higher values in pre-hibernation than those of the breeding period. Strong immunostaining of TLR4, MyD88, IRAK4 and NF-κΒ were observed in the pre-hibernation. These proteins levels had no significant difference between these two periods except MyD88 which was significantly higher in the pre-hibernation. The mRNA expression levels of MyD88 and NF-κΒ were obviously higher in pre-hibernation than those of the breeding period. These findings suggested that TLR4/MyD88 signal pathway might participate in the oviductal functions of Rana dybowskii during the breeding period and pre-hibernation.https://www.ejh.it/index.php/ejh/article/view/3050Rana dybowskiioviductMyD88TLR4IRAK4NF-κΒ |
| spellingShingle | Yue Zhang Ao Zhang Yue Zhao Xiaohang Feng Yuyao Sheng Haolin Zhang Qiang Weng Meiyu Xu Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii) European Journal of Histochemistry Rana dybowskii oviduct MyD88 TLR4 IRAK4 NF-κΒ |
| title | Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii) |
| title_full | Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii) |
| title_fullStr | Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii) |
| title_full_unstemmed | Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii) |
| title_short | Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii) |
| title_sort | expressions of tlr4 myd88 irak4 and nf κβ in the oviduct of chinese brown frog rana dybowskii |
| topic | Rana dybowskii oviduct MyD88 TLR4 IRAK4 NF-κΒ |
| url | https://www.ejh.it/index.php/ejh/article/view/3050 |
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