REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS
Objectives: This study aimed to identify porcine DNA in prosthodontic materials using Real-Time PCR. Materials and methods: Eighteen prosthodontic materials: three irreversible hydrocolloids, three elastomers, three denture adhesives, three soft denture linings, three temporary crowns, and three d...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Cumhuriyet University
2021-09-01
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| Series: | Cumhuriyet Dental Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/1603099 |
| _version_ | 1797703774439997440 |
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| author | Ismail Hutasoit Agy Pranata Masna Wiyah Fitri Ramadhani Hubban Nasution |
| author_facet | Ismail Hutasoit Agy Pranata Masna Wiyah Fitri Ramadhani Hubban Nasution |
| author_sort | Ismail Hutasoit |
| collection | DOAJ |
| description | Objectives: This study aimed to identify porcine DNA in prosthodontic materials using Real-Time PCR.
Materials and methods: Eighteen prosthodontic materials: three irreversible hydrocolloids, three elastomers, three denture adhesives, three soft denture linings, three temporary crowns, and three denture bases materials were used as the samples. It was conducted in two stages. First, extraction of dental material’s DNA and the second was Real-Time PCR analysis based on amplification curve and Ct score in yellow and green channel.
Results: The sample analysis based on green channel demonstrated that all materials did not contain porcine DNA, however, 11 of 18 samples (DA-01, DA-02, DA-03, SDL-02, DB-01, DB-02, TC-03, IH-02, EM-01, EM-02, and EM-03) contained vertebrate DNA. Conclusions: All prosthodontic materials tested were not containing porcine. The halal statues of the materials were still unclear. |
| first_indexed | 2024-03-12T05:09:26Z |
| format | Article |
| id | doaj.art-29b0f62492dd4954bed2b9801c740f1c |
| institution | Directory Open Access Journal |
| issn | 1302-5805 2146-2852 |
| language | English |
| last_indexed | 2024-03-12T05:09:26Z |
| publishDate | 2021-09-01 |
| publisher | Cumhuriyet University |
| record_format | Article |
| series | Cumhuriyet Dental Journal |
| spelling | doaj.art-29b0f62492dd4954bed2b9801c740f1c2023-09-03T08:41:51ZengCumhuriyet UniversityCumhuriyet Dental Journal1302-58052146-28522021-09-0124321622310.7126/cumudj.8871012057REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALSIsmail Hutasoit0Agy Pranata1Masna Wiyah2Fitri Ramadhani3Hubban Nasution4Universitas Sumatera UtaraInstitut Pertanian BogorUniversitas Sumatera UtaraUniversitas Sumatera UtaraUniversitas Sumatera UtaraObjectives: This study aimed to identify porcine DNA in prosthodontic materials using Real-Time PCR. Materials and methods: Eighteen prosthodontic materials: three irreversible hydrocolloids, three elastomers, three denture adhesives, three soft denture linings, three temporary crowns, and three denture bases materials were used as the samples. It was conducted in two stages. First, extraction of dental material’s DNA and the second was Real-Time PCR analysis based on amplification curve and Ct score in yellow and green channel. Results: The sample analysis based on green channel demonstrated that all materials did not contain porcine DNA, however, 11 of 18 samples (DA-01, DA-02, DA-03, SDL-02, DB-01, DB-02, TC-03, IH-02, EM-01, EM-02, and EM-03) contained vertebrate DNA. Conclusions: All prosthodontic materials tested were not containing porcine. The halal statues of the materials were still unclear.https://dergipark.org.tr/tr/download/article-file/1603099pcrporcine dnaprosthodontic materials |
| spellingShingle | Ismail Hutasoit Agy Pranata Masna Wiyah Fitri Ramadhani Hubban Nasution REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS Cumhuriyet Dental Journal pcr porcine dna prosthodontic materials |
| title | REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS |
| title_full | REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS |
| title_fullStr | REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS |
| title_full_unstemmed | REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS |
| title_short | REAL-TIME PCR TO IDENTIFY PORCINE DNA IN PROSTHODONTIC MATERIALS |
| title_sort | real time pcr to identify porcine dna in prosthodontic materials |
| topic | pcr porcine dna prosthodontic materials |
| url | https://dergipark.org.tr/tr/download/article-file/1603099 |
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