Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds

Although Newcastle disease virus (NDV) with high pathogenicity has frequently been isolated in poultry in China since 1948, the mode of its transmission among avian species remains largely unknown. Given that various wild bird species have been implicated as sources of transmission, in this study we...

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Main Authors: Yinfeng eKang, Bin eXiang, Runyu eYuan, Xiaqiong eZhao, Minsha eFeng, Pei eGao, Yanling eLi, Yulian eLi, Zhangyong eNing, Tao eRen
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-02-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00119/full
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author Yinfeng eKang
Yinfeng eKang
Bin eXiang
Bin eXiang
Runyu eYuan
Runyu eYuan
Runyu eYuan
Xiaqiong eZhao
Xiaqiong eZhao
Minsha eFeng
Minsha eFeng
Pei eGao
Pei eGao
Yanling eLi
Yanling eLi
Yulian eLi
Yulian eLi
Zhangyong eNing
Tao eRen
Tao eRen
author_facet Yinfeng eKang
Yinfeng eKang
Bin eXiang
Bin eXiang
Runyu eYuan
Runyu eYuan
Runyu eYuan
Xiaqiong eZhao
Xiaqiong eZhao
Minsha eFeng
Minsha eFeng
Pei eGao
Pei eGao
Yanling eLi
Yanling eLi
Yulian eLi
Yulian eLi
Zhangyong eNing
Tao eRen
Tao eRen
author_sort Yinfeng eKang
collection DOAJ
description Although Newcastle disease virus (NDV) with high pathogenicity has frequently been isolated in poultry in China since 1948, the mode of its transmission among avian species remains largely unknown. Given that various wild bird species have been implicated as sources of transmission, in this study we genotypically and pathotypically characterized 23 NDV isolates collected from chickens, ducks, and pigeons in live bird markets (LBMs) in South China as part of an H7N9 surveillance program during December 2013–February 2014. To simulate the natural transmission of different kinds of animals in LBMs, we selected three representative NDVs—namely, GM, YF18, and GZ289—isolated from different birds to evaluate the pathogenicity and transmission of the indicated viruses in chickens, ducks, and pigeons. Furthermore, to investigate the replication and shedding of NDV in poultry, we inoculated the chickens, ducks, and pigeons with 106 EID50 of each virus via intraocular and intranasal routes. Eight h after infection, the naïve contact groups were housed with those inoculated with each of the viruses as a means to monitor contact transmission. Our results indicated that genetically diverse viruses circulate in LBMs in South China’s Guangdong Province and that NDV from different birds have different tissue tropisms and host ranges when transmitted in different birds. We therefore propose the continuous epidemiological surveillance of LBMs to support the prevention of the spread of these viruses in different birds, especially chickens, and highlight the need for studies of the virus–host relationship.
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spelling doaj.art-29b4b35023cd4652a9eb30b540cbb6cb2022-12-22T01:26:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-02-01710.3389/fmicb.2016.00119177304Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birdsYinfeng eKang0Yinfeng eKang1Bin eXiang2Bin eXiang3Runyu eYuan4Runyu eYuan5Runyu eYuan6Xiaqiong eZhao7Xiaqiong eZhao8Minsha eFeng9Minsha eFeng10Pei eGao11Pei eGao12Yanling eLi13Yanling eLi14Yulian eLi15Yulian eLi16Zhangyong eNing17Tao eRen18Tao eRen19South China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangdong Provincial Center for Disease Control and PreventionSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceSouth China Agricultural UniversitySouth China Agricultural UniversityKey Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceAlthough Newcastle disease virus (NDV) with high pathogenicity has frequently been isolated in poultry in China since 1948, the mode of its transmission among avian species remains largely unknown. Given that various wild bird species have been implicated as sources of transmission, in this study we genotypically and pathotypically characterized 23 NDV isolates collected from chickens, ducks, and pigeons in live bird markets (LBMs) in South China as part of an H7N9 surveillance program during December 2013–February 2014. To simulate the natural transmission of different kinds of animals in LBMs, we selected three representative NDVs—namely, GM, YF18, and GZ289—isolated from different birds to evaluate the pathogenicity and transmission of the indicated viruses in chickens, ducks, and pigeons. Furthermore, to investigate the replication and shedding of NDV in poultry, we inoculated the chickens, ducks, and pigeons with 106 EID50 of each virus via intraocular and intranasal routes. Eight h after infection, the naïve contact groups were housed with those inoculated with each of the viruses as a means to monitor contact transmission. Our results indicated that genetically diverse viruses circulate in LBMs in South China’s Guangdong Province and that NDV from different birds have different tissue tropisms and host ranges when transmitted in different birds. We therefore propose the continuous epidemiological surveillance of LBMs to support the prevention of the spread of these viruses in different birds, especially chickens, and highlight the need for studies of the virus–host relationship.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00119/fullNewcastle disease virus (NDV);Phylogenetic analysis;Pathogenicity;Transmission;South China;
spellingShingle Yinfeng eKang
Yinfeng eKang
Bin eXiang
Bin eXiang
Runyu eYuan
Runyu eYuan
Runyu eYuan
Xiaqiong eZhao
Xiaqiong eZhao
Minsha eFeng
Minsha eFeng
Pei eGao
Pei eGao
Yanling eLi
Yanling eLi
Yulian eLi
Yulian eLi
Zhangyong eNing
Tao eRen
Tao eRen
Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds
Frontiers in Microbiology
Newcastle disease virus (NDV);
Phylogenetic analysis;
Pathogenicity;
Transmission;
South China;
title Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds
title_full Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds
title_fullStr Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds
title_full_unstemmed Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds
title_short Phylogenetic and pathotypic characterization of Newcastle disease viruses circulating in South China and transmission in different birds
title_sort phylogenetic and pathotypic characterization of newcastle disease viruses circulating in south china and transmission in different birds
topic Newcastle disease virus (NDV);
Phylogenetic analysis;
Pathogenicity;
Transmission;
South China;
url http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00119/full
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