Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation
Abstract Endosymbionts are known to have significant effects on their insect hosts, including nutrition, reproduction, and immunity. Insects gut microbiota is a critical component that affects their physiological and behavioral characteristics. The black cutworm (BCW), Agrotis ipsilon, is an economi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Animal Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42523-023-00264-6 |
| _version_ | 1797556144548347904 |
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| author | Omnia Abdullah ElKraly Mona Awad Hassan Mohamed El-Saadany Sameh E. Hassanein Tahany Abd Elrahman Sherif M. Elnagdy |
| author_facet | Omnia Abdullah ElKraly Mona Awad Hassan Mohamed El-Saadany Sameh E. Hassanein Tahany Abd Elrahman Sherif M. Elnagdy |
| author_sort | Omnia Abdullah ElKraly |
| collection | DOAJ |
| description | Abstract Endosymbionts are known to have significant effects on their insect hosts, including nutrition, reproduction, and immunity. Insects gut microbiota is a critical component that affects their physiological and behavioral characteristics. The black cutworm (BCW), Agrotis ipsilon, is an economically important lepidopteran pest that has a diverse gut microbiome composed of nine species belonging to three phyla: Proteobacteria, Actinobacteria, and Firmicutes. This study was conducted to investigate the diversity of gut bacteria isolated from BCW larvae and moths and their effects on metabolism and pesticide degradation. The bacterial isolates were identified using the 16 S rRNA gene. The study showed that the gut microbiome composition significantly affected the metabolism of BCW larvae. Based on the screening results of synthesis of digestive enzymes and pesticide degradation, Brachybacterium conglomeratum and Glutamicibacter sp were selected to perform the remaining experiments as single isolates and consortium. The consortium-fed larvae showed high metabolic indices compared to antibiotic-fed larvae and the control. The gut bacteria were also shown to degrade three pesticide groups. Concerns regarding the health risk of chlorpyrifos have been raised due to its extensive use in agriculture. The isolated B. conglomeratum was more effective in chlorpyrifos degradation than the consortium. Furthermore, the study also examined the presence of sex related endosymbionts (Wolbachia, Spiroplasma, and Rickettsia) in the reproductive tissues of adults. The outcomes demonstrated that none of the examined endosymbionts existed. In conclusion, the study highlights the importance of the gut microbiome in insect physiology and behavior and its potential applications in biotechnology. It provides insights into developing eco-friendly pest control and bioremediation strategies using gut bacteria. |
| first_indexed | 2024-03-10T16:58:41Z |
| format | Article |
| id | doaj.art-29b65fa7df3e4f5ba0a48675d072a412 |
| institution | Directory Open Access Journal |
| issn | 2524-4671 |
| language | English |
| last_indexed | 2024-03-10T16:58:41Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Microbiome |
| spelling | doaj.art-29b65fa7df3e4f5ba0a48675d072a4122023-11-20T11:03:09ZengBMCAnimal Microbiome2524-46712023-09-015111410.1186/s42523-023-00264-6Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradationOmnia Abdullah ElKraly0Mona Awad1Hassan Mohamed El-Saadany2Sameh E. Hassanein3Tahany Abd Elrahman4Sherif M. Elnagdy5Botany and Microbiology Department, Faculty of Science, Cairo UniversityDepartment of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo UniversityBio-insecticides Production Unit, Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Ministry of AgricultureCollege of Biotechnology, Misr University for Science and Technology (MUST)Botany and Microbiology Department, Faculty of Science, Cairo UniversityBotany and Microbiology Department, Faculty of Science, Cairo UniversityAbstract Endosymbionts are known to have significant effects on their insect hosts, including nutrition, reproduction, and immunity. Insects gut microbiota is a critical component that affects their physiological and behavioral characteristics. The black cutworm (BCW), Agrotis ipsilon, is an economically important lepidopteran pest that has a diverse gut microbiome composed of nine species belonging to three phyla: Proteobacteria, Actinobacteria, and Firmicutes. This study was conducted to investigate the diversity of gut bacteria isolated from BCW larvae and moths and their effects on metabolism and pesticide degradation. The bacterial isolates were identified using the 16 S rRNA gene. The study showed that the gut microbiome composition significantly affected the metabolism of BCW larvae. Based on the screening results of synthesis of digestive enzymes and pesticide degradation, Brachybacterium conglomeratum and Glutamicibacter sp were selected to perform the remaining experiments as single isolates and consortium. The consortium-fed larvae showed high metabolic indices compared to antibiotic-fed larvae and the control. The gut bacteria were also shown to degrade three pesticide groups. Concerns regarding the health risk of chlorpyrifos have been raised due to its extensive use in agriculture. The isolated B. conglomeratum was more effective in chlorpyrifos degradation than the consortium. Furthermore, the study also examined the presence of sex related endosymbionts (Wolbachia, Spiroplasma, and Rickettsia) in the reproductive tissues of adults. The outcomes demonstrated that none of the examined endosymbionts existed. In conclusion, the study highlights the importance of the gut microbiome in insect physiology and behavior and its potential applications in biotechnology. It provides insights into developing eco-friendly pest control and bioremediation strategies using gut bacteria.https://doi.org/10.1186/s42523-023-00264-6Agrotis ipsilonEndosymbiontsGut bacteriaInsecticide degradationMetabolism |
| spellingShingle | Omnia Abdullah ElKraly Mona Awad Hassan Mohamed El-Saadany Sameh E. Hassanein Tahany Abd Elrahman Sherif M. Elnagdy Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation Animal Microbiome Agrotis ipsilon Endosymbionts Gut bacteria Insecticide degradation Metabolism |
| title | Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation |
| title_full | Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation |
| title_fullStr | Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation |
| title_full_unstemmed | Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation |
| title_short | Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation |
| title_sort | impact of gut microbiota composition on black cutworm agrotis ipsilon hufnagel metabolic indices and pesticide degradation |
| topic | Agrotis ipsilon Endosymbionts Gut bacteria Insecticide degradation Metabolism |
| url | https://doi.org/10.1186/s42523-023-00264-6 |
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