Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors
The design of multimodal imaging nanoplatforms with improved tumor accumulation represents a major trend in the current development of precision nanomedicine. To this end, we report herein the preparation of macrophage (MA)-laden gold nanoflowers (NFs) embedded with ultrasmall iron oxide nanoparticl...
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MDPI AG
2021-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/7/995 |
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| author | Yucheng Peng Xiaomeng Wang Yue Wang Yue Gao Rui Guo Xiangyang Shi Xueyan Cao |
| author_facet | Yucheng Peng Xiaomeng Wang Yue Wang Yue Gao Rui Guo Xiangyang Shi Xueyan Cao |
| author_sort | Yucheng Peng |
| collection | DOAJ |
| description | The design of multimodal imaging nanoplatforms with improved tumor accumulation represents a major trend in the current development of precision nanomedicine. To this end, we report herein the preparation of macrophage (MA)-laden gold nanoflowers (NFs) embedded with ultrasmall iron oxide nanoparticles (USIO NPs) for enhanced dual-mode computed tomography (CT) and magnetic resonance (MR) imaging of tumors. In this work, generation 5 poly(amidoamine) (G5 PAMAM) dendrimer-stabilized gold (Au) NPs were conjugated with sodium citrate-stabilized USIO NPs to form hybrid seed particles for the subsequent growth of Au nanoflowers (NFs). Afterwards, the remaining terminal amines of dendrimers were acetylated to form the dendrimer-stabilized Fe<sub>3</sub>O<sub>4</sub>/Au NFs (for short, Fe<sub>3</sub>O<sub>4</sub>/Au DSNFs). The acquired Fe<sub>3</sub>O<sub>4</sub>/Au DSNFs possess an average size around 90 nm, display a high r<sub>1</sub> relaxivity (1.22 mM<sup>−1</sup> s<sup>−1</sup>), and exhibit good colloidal stability and cytocompatibility. The created hybrid DSNFs can be loaded within MAs without producing any toxicity to the cells. Through the mediation of MAs with a tumor homing and immune evasion property, the Fe<sub>3</sub>O<sub>4</sub>/Au DSNFs can be delivered to tumors more efficiently than those without MAs after intravenous injection, thus significantly improving the MR/CT imaging performance of tumors. The developed MA-mediated delivery system may hold great promise for enhanced tumor delivery of other contrast agents or nanomedicines for precision cancer nanomedicine applications. |
| first_indexed | 2024-03-10T09:53:31Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T09:53:31Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-29ba183c58f44142844f9f7512befa1d2023-11-22T02:29:28ZengMDPI AGPharmaceutics1999-49232021-06-0113799510.3390/pharmaceutics13070995Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of TumorsYucheng Peng0Xiaomeng Wang1Yue Wang2Yue Gao3Rui Guo4Xiangyang Shi5Xueyan Cao6Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, ChinaShanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, ChinaDepartment of Radiology, Shanghai Songjiang District Central Hospital, Shanghai 201600, ChinaShanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, ChinaShanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, ChinaShanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, ChinaShanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, ChinaThe design of multimodal imaging nanoplatforms with improved tumor accumulation represents a major trend in the current development of precision nanomedicine. To this end, we report herein the preparation of macrophage (MA)-laden gold nanoflowers (NFs) embedded with ultrasmall iron oxide nanoparticles (USIO NPs) for enhanced dual-mode computed tomography (CT) and magnetic resonance (MR) imaging of tumors. In this work, generation 5 poly(amidoamine) (G5 PAMAM) dendrimer-stabilized gold (Au) NPs were conjugated with sodium citrate-stabilized USIO NPs to form hybrid seed particles for the subsequent growth of Au nanoflowers (NFs). Afterwards, the remaining terminal amines of dendrimers were acetylated to form the dendrimer-stabilized Fe<sub>3</sub>O<sub>4</sub>/Au NFs (for short, Fe<sub>3</sub>O<sub>4</sub>/Au DSNFs). The acquired Fe<sub>3</sub>O<sub>4</sub>/Au DSNFs possess an average size around 90 nm, display a high r<sub>1</sub> relaxivity (1.22 mM<sup>−1</sup> s<sup>−1</sup>), and exhibit good colloidal stability and cytocompatibility. The created hybrid DSNFs can be loaded within MAs without producing any toxicity to the cells. Through the mediation of MAs with a tumor homing and immune evasion property, the Fe<sub>3</sub>O<sub>4</sub>/Au DSNFs can be delivered to tumors more efficiently than those without MAs after intravenous injection, thus significantly improving the MR/CT imaging performance of tumors. The developed MA-mediated delivery system may hold great promise for enhanced tumor delivery of other contrast agents or nanomedicines for precision cancer nanomedicine applications.https://www.mdpi.com/1999-4923/13/7/995gold nanoflowersultrasmall iron oxide nanoparticlesmultimode imagingmacrophagetumor diagnosis |
| spellingShingle | Yucheng Peng Xiaomeng Wang Yue Wang Yue Gao Rui Guo Xiangyang Shi Xueyan Cao Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors Pharmaceutics gold nanoflowers ultrasmall iron oxide nanoparticles multimode imaging macrophage tumor diagnosis |
| title | Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors |
| title_full | Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors |
| title_fullStr | Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors |
| title_full_unstemmed | Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors |
| title_short | Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors |
| title_sort | macrophage laden gold nanoflowers embedded with ultrasmall iron oxide nanoparticles for enhanced dual mode ct mr imaging of tumors |
| topic | gold nanoflowers ultrasmall iron oxide nanoparticles multimode imaging macrophage tumor diagnosis |
| url | https://www.mdpi.com/1999-4923/13/7/995 |
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