| Summary: | Newcastle disease virus (NDV) was isolated from a field outbreak in pigeons. The virus was characterized by haemagglutination test (HA) and confirmed by haemagglutination inhibition test (HAI). The pathotyping was done by mean death time (MDT), intracerebral pathogenicity index (ICPI) and intravenous pathogenicity index (IVPI). The ELD50 of the velogenic strain was 10-4.66/0.1 ml. Thirty-nine pigeons were randomly divided into three equal groups. Pigeons of one group were vaccinated with ND vaccine (LaSota strain) intraocularly after 14 days of procurement, while the other two groups served as vaccinated and non-vaccinated controls. Birds of these two groups were challenged with velogenic strain of field isolate of NDV 7 days post-vaccination. Birds were kept under observation for 15 days post-challenge. Haemorrhages and congestion were observed in trachea, lungs, liver, proventriculus and intestine of pigeons infected with NDV. Concentrations of AST, ALT and ALP did not differ among pigeons of the three groups.
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